FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490459
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332236
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN29360.8837091705895809No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12200.36720885153926724No Hit
CTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA11100.3340998567283497No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA9670.2910581634741569No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG8890.2675808762445972No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGCCCCACAATTCTTTGACATACT7780.23417089057176224No Hit
GTTTTAGTACAATGTGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACA6840.2058777495515236No Hit
GGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT6810.20497477696577132No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACAGTGCTGAGAGTCCAA6780.20407180438001904No Hit
TTCTAATACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCGCCTTTC6380.19203216990332173No Hit
CCCTAGCAGCCATGGGAAGGAGGTGTATTTCCGTGAGAGGACAACAGAGT6260.18842027956031254No Hit
GTATACATGCATATAAAGGCATCAAAGCAGGATAGCCACATTGTGTAAAA6100.18360442576963362No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTATTCCCTTAACTTCAT5940.1787885719789547No Hit
GGTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT5870.17668163594553268No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG5850.17607965422169783No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT5580.16795290094992715No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5530.16644794664034No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCACTGTCAACGACCGACCT5530.16644794664034No Hit
GTATTAGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGGAT5520.1661469557784226No Hit
GTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTTAAGGACT5380.1619330837115785No Hit
GTACTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT5370.1616320928496611No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5250.15802020250665189No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT5090.15320434871597297No Hit
CTTTTACACAATGTGGCTATCCTGCTTTGATGCCTTTATATGCATGTATA5070.1526023669921381No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAGTACAATG4980.14989344923488124No Hit
GTACGGGACCATGCAAAACCTGCACGATTCCTGCTCAAGGAACCTCTATG4930.14838849492529407No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG4900.1474855223395418No Hit
GTCGTTGACAGTGCTGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG4860.14628155889187203No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4840.14567957716803717No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAATAAG4750.1429706594107803No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4740.14266966854886287No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCCGGTCTCTGCCAAGTGTTTGCTG4630.1393587690677711No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGCCCCACAAT4600.1384557964820188No Hit
GTACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTATG4440.13363994269133989No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA4410.1327369701055876No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4310.12972706148641328No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC4280.128824088900661No Hit
GGCCTCTACCGTCCCCTTCTTCATCTGCCGTTCCGGCCGACCACGGGGCG4260.1282221071768261No Hit
TTCTTAATAGATGTCAACAATTTGTGGGCCCTCTTACAGTTAATGAAAAA4250.12792111631490868No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC4210.12671715286723895No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC4180.12581418028148666No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAA4170.1255131894195692No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG4080.12280427166231234No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG4050.12190129907656004No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA4050.12190129907656004No Hit
GTCCCGTACTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC4050.12190129907656004No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG4000.12039634476697288No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC3990.12009535390505545No Hit
ATGTGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA3990.12009535390505545No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAGTACAAT3970.11949337218122057No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG3950.11889139045738573No Hit
CATATAAAGGCATCAAAGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA3850.1158814818382114No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC3830.11527950011437653No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA3810.11467751839054166No Hit
CTGTAAGAGGGCCCACAAATTGTTGACATCTATTAAGAATGTCCTCCTGT3710.11166760977136733No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT3650.10986166459986274No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG3630.10925968287602789No Hit
GTAGAGGCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG3590.10805571942835816No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATATC3590.10805571942835816No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTAC3550.10685175598068841No Hit
GTCTTTGGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTATT3460.10414283822343154No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC3450.1038418473615141No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG3440.10354085649959667No Hit
GTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTATTCCCTTAAC3440.10354085649959667No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAGTACAATGTGTT3420.1029388747757618No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC3420.1029388747757618No Hit
CACACAATAGCTTGCCTGAGTGCTGTATGGTGAGGTGAACAATGTTCCGG3400.10233689305192695No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC3360.10113292960425722No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCCGGTC3360.10113292960425722No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC3330.10022995701850493No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)