FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490460

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490460
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences261022
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAACAAG11390.4363616859881543No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10470.401115614775766No Hit
GTCCTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA9260.3547593689420815No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGCCCCACAATTCTTTGACATACT7740.296526729547701No Hit
GTACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG7380.28273478863850554No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACAGTACTGAGAGTCCAA7190.275455708714208No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC6920.26511175303231144No Hit
GTACCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6390.24480695113821824No Hit
CATGTATACAAGCTAAGCAGGCTTTTACTTTCTCGCCAACTTACAAGGCC6190.2371447617442208No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT5840.22373593030472527No Hit
GTATACATGCATATAAAGGCATCAAAGCAGGATAGCCACATTGTGTAAAA5630.21569063144102796No Hit
GGCTTTTACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATATC5590.21415819356222845No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG5570.21339197462282872No Hit
GCCATGGGAAGGAGGTGTATTTCCGTGAGAGGACAACAGAGTTGTCAGTC5510.21109331780462948No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC5500.21071020833492962No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC5350.20496356628943155No Hit
GATTTAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA5310.20343112841063204No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTATTCCCTTAACTTCAT5220.1999831431833332No Hit
CTTTTACACAATGTGGCTATCCTGCTTTGATGCCTTTATATGCATGTATA5200.19921692424393345No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG5200.19921692424393345No Hit
GTACTGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGG5030.19270406325903564No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5000.19155473484993604No Hit
GACTTCTTTCCTTCTATTCGAGATCTCCTCGACACCGCCTCTGCTCTGTA4980.19078851591053628No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTATTCCCTTAACTTCAT4920.18848985909233704No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG4850.18580809280443794No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTAC4710.18044456022863975No Hit
GTACTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT4710.18044456022863975No Hit
GAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAATCTTGCGAAAG4700.18006145075893987No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG4630.17737968447104074No Hit
GTTTTAGTACAATGTGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACA4580.1754641371225414No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG4580.1754641371225414No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4550.1743148087134418No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC4520.17316548030434217No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG4480.17163304242554267No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT4450.17048371401644308No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGCCCCACAAT4450.17048371401644308No Hit
GCTCTGTACCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA4380.16780194772854395No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4220.161672196213346No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG4210.16128908674364612No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT4200.16090597727394626No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC4200.16090597727394626No Hit
GTAGAGGCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG4190.16052286780424638No Hit
GTCGTTGACAGTACTGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG4170.15975664886484664No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAGTACAATG4130.15822421098604716No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC4080.15630866363754778No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4060.15554244469814804No Hit
GGATGGGGCTTGGCCATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGC4030.15439311628904845No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4030.15439311628904845No Hit
CATATAAAGGCATCAAAGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA4020.15401000681934857No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4010.15362689734964868No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG3980.15247756894054906No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG3920.15017891212234985No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC3900.1494126931829501No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG3870.1482633647738505No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC3810.14596470795565125No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG3800.14558159848595137No Hit
CCTCAAGGTCGGTCGTTGACAGTACTGAGAGTCCAAGAGTCCTCTTATGT3730.14289983219805227No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG3720.1425167227283524No Hit
GACGGGGAGACCGCGTAAAGAGAGGTACGCCCCGTGGTCGGCCGGAACGG3620.13868562803135367No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAA3610.13830251856165382No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAA3600.13791940909195394No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC3580.1371531901525542No Hit
GCCTATGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGC3570.13677008068285432No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAATTACTCTCTTTT3520.13485453333435496No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGTACTGTCAACGACCGACCT3490.13370520492525534No Hit
GGCCTCTACCGTCCCCTTCTTCATCTGCCGTTCCGGCCGACCACGGGGCG3460.13255587651615572No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG3440.13178965757675598No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA3430.1314065481070561No Hit
GTACAAACAAGACCTTTAACCTGGTATCCTCCCCCAACTCCTCCCAGTCC3430.1314065481070561No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG3420.13102343863735624No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG3330.12757545341005738No Hit
CAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAGT3330.12757545341005738No Hit
TCCTAGCTCAGTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTC3310.12680923447065764No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG3280.12565990606155802No Hit
GATCCATACTGCGGAACTCCTAGCAGCTTGTTTTGCTCGCAGCCGGTCTG3240.12412746818275854No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC3240.12412746818275854No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAATTACTCTCTTTTTTGCCTTCTG3240.12412746818275854No Hit
CTCTAAGGCCTCCCGGTACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA3200.12259503030395906No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAGTACAAT3190.12221192083425919No Hit
GTCCGTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA3180.12182881136455931No Hit
GCCATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGCTCCTCTGCCGAT3140.12029637348575982No Hit
GTCCCGTACTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC3140.12029637348575982No Hit
GCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGCAGAGACCTGACC3130.11991326401605995No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC3080.11799771666756058No Hit
CTTTATATGCATGTATACAAGCTAAGCAGGCTTTTACTTTCTCGCCAACT3080.11799771666756058No Hit
CCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGACCCGTAT3050.11684838825846097No Hit
GACAAAGGACGTCCCGCGCAGGATCCAGTTGGCAGCACACCCGAGCAGCC3050.11684838825846097No Hit
GGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT3030.11608216931906123No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG3030.11608216931906123No Hit
GGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAA3020.11569905984936135No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAGTACAATGTGTT3010.11531595037966148No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT3000.11493284090996161No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG3000.11493284090996161No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT2990.11454973144026173No Hit
GTGGGGGAAAGCCCTGCGAACCACTGAACAAATGGCACTAGTAAACTGAG2990.11454973144026173No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAACAAGACC2970.113783512500862No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA2970.113783512500862No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC2970.113783512500862No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA2960.11340040303116211No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA2960.11340040303116211No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC2960.11340040303116211No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAGGGCATTAAACCTTAT2950.11301729356146224No Hit
TCCCGGTACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA2950.11301729356146224No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA2940.11263418409176239No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCTAAGCAGGCTTTTACTTT2930.11225107462206252No Hit
GCTTTGATGCCTTTATATGCATGTATACAAGCTAAGCAGGCTTTTACTTT2870.10995241780386328No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA2870.10995241780386328No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAA2860.1095693083341634No Hit
CTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA2850.10918619886446353No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA2840.10880308939476366No Hit
GCTTGTATACATGCATATAAAGGCATCAAAGCAGGATAGCCACATTGTGT2830.10841997992506378No Hit
GTACAAAGACCTTTAACCTGGTATCCTCCCCCAACTCCTCCCAGTCCTTA2820.1080368704553639No Hit
GCACCAAACCTCGACAAGGCATGGGGACGAATCTTTCTGTTCCCAATCCT2810.10765376098566405No Hit
TATACAAGCTAAGCAGGCTTTTACTTTCTCGCCAACTTACAAGGCCTTTC2790.1068875420462643No Hit
ATTTATGCCTACAGCCTCCTAGTACAAACAAGACCTTTAACCTGGTATCC2750.10535510416746481No Hit
CCTCTGCTCTGTACCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCT2750.10535510416746481No Hit
ATGTTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG2700.10343955681896544No Hit
ATGTGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA2700.10343955681896544No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCGAAAGTAAGA2680.10267333787956572No Hit
CGTAAAGAGAGGTACGCCCCGTGGTCGGCCGGAACGGCAGATGAAGAAGG2620.10037468106136647No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)