FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002490463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences517354
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC22380.4325858116492769No Hit
GTATACATGCATATAAAGGCATCAAGGCGGGATAGCCACATTGTGTAAAA18400.355655895189754No Hit
GTGTAAAAGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT17020.3289817030505225No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA15780.3050135883746912No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG15500.2996014334478906No Hit
CATGTATACAATCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC15390.2974752297266475No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC15090.2916764923050754No Hit
TCATTAACCTGATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAG14630.2827850949253316No Hit
GACCTTTAACCTGATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAAC14620.28259180367794584No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC13530.26152305771290063No Hit
GCCTAGTCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC13220.25553102904394287No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG12020.23233607935765452No Hit
GATCATTAACCTGATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAAC11670.22557088569915376No Hit
GCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA11530.22286480823575347No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC11510.22247822574098197No Hit
GTATTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT11340.21919227453542448No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA10570.20430884848672282No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAATACAATA10030.1938711211278931No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG9990.19309795613835015No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG9920.19174491740664998No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG9830.1900052961801784No Hit
CCTTTTACACAATGTGGCTATCCCGCCTTGATGCCTTTATATGCATGTAT9660.18671934497462087No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT9650.18652605372723513No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA9620.1859461799850779No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC9490.18343339376906334No Hit
CATATAAAGGCATCAAGGCGGGATAGCCACATTGTGTAAAAGGAGCAGCA9430.18227364628474893No Hit
CTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA9210.17802123884226273No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG9180.17744136510010555No Hit
GTTTTAATACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACA8770.16951642395729036No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC8710.16835667647297597No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA8620.16661705524650433No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG8590.16603718150434713No Hit
AGTTAAGGGAGTAGCCCCAGCGTTTGGTTTTATTAGGGTTCAAATGTATA8580.1658438902569614No Hit
GGGTAAAGGTGCAGGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA8520.16468414277264698No Hit
CATTTGAACCCTAATAAAACCAAACGCTGGGGCTACTCCCTTAACTTCAT8460.16352439528833257No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTT8360.1615914828144752No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG8260.15965857034061784No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA8200.15849882285630343No Hit
GGACTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCATTG8160.15772565786676046No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC8020.15501958040336017No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG7980.15424641541381723No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG7840.1515403379504169No Hit
GGAACATATTGTATTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAA7780.1503805904661025No Hit
GTAGAGGCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG7700.14883426048701662No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG7660.1480610954974737No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7660.1480610954974737No Hit
TGGAAACCACCGTGAACGCCCACCAGGTCTTGCCCAAGCTCTTACATAAG7600.14690134801315927No Hit
GGATGGGGCTTGGCCATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGC7590.14670805676577353No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG7570.14632147427100206No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAA7450.1440019793023732No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC7330.14168248433374442No Hit
GCCTATGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGC7280.14071602809681572No Hit
GTTTAGATTGTATACATGCATATAAAGGCATCAAGGCGGGATAGCCACAT7240.13994286310727277No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA7220.1395562806125013No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAATACAAT7220.1395562806125013No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG7210.13936298936511557No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC7070.13665691190171528No Hit
GTTTAGCTCCAGACCGGCTGCGAGCAAAACAAGCTGCTAGGAGTTCCGCA7000.13530387317001513No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG6940.13414412568570072No Hit
GTCCAAGAGTCCTCTTATGTAAGAGCTTGGGCAAGACCTGGTGGGCGTTC6920.13375754319092922No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT6820.13182463071707187No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6800.1314380482223004No Hit
TTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA6800.1314380482223004No Hit
GTCCCGTGCTGGTAGTTGATGTTCATTGAGATTCCCGAGATTGAGATCTT6770.1308581744801432No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTGATCTCCTCC6720.1298917182432145No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC6700.12950513574844305No Hit
CAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAAT6670.12892526200628585No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAATACAATATGTT6620.12795880576935717No Hit
GGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAG6610.12776551452197143No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT6590.12737893202719994No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT6570.12699234953242847No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC6540.12641247579027126No Hit
GTGGGGGAAAGCCCTGCGAACCACTGAACAAATGGCACTAGTAAACTGAG6530.12621918454288553No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA6510.12583260204811406No Hit
CCCTCATAAAACCAAACGCTGGGGCTACTCCCTTAACTTCATGGGATATG6490.12544601955334259No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG6450.12467285456379965No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC6430.12428627206902818No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG6420.12409298082164244No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6390.12351310707948522No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA6350.12273994208994228No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA6270.1211936121108564No Hit
TCCTAGTACAAAGATCATTAACCTGATCTCCTCCCCCAACTCCTCCCAGT6200.11984057337915625No Hit
ATCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTC6170.11926069963699903No Hit
GCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGCAGAGACCTGACC6160.1190674083896133No Hit
GTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGTAAGAGCTTGGG6160.1190674083896133No Hit
ATACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC6140.11868082589484184No Hit
TAGCCACATTGTGTAAAAGGAGCAGCAAAGCCCAAAAGACCCACAATTCT6140.11868082589484184No Hit
GTATGGTGAGGTGAACAATGTTCCGGAGACTCTAAGGCCTCCCGATACAG6090.11771436965791315No Hit
CTTTATATGCATGTATACAATCTAAACAGGCTTTCACTTTCTCGCCAACT6080.1175210784105274No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG6040.11674791342098448No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT5980.11558816593667007No Hit
GTATACATTTGAACCCTAATAAAACCAAACGCTGGGGCTACTCCCTTAAC5950.11500829219451285No Hit
GCCATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGCTCCTCTGCCGAT5930.11462170969974138No Hit
CCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCC5930.11462170969974138No Hit
CCTTTATATGCATGTATACAATCTAAACAGGCTTTCACTTTCTCGCCAAC5860.11326867096804122No Hit
CTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAGGA5820.11249550597849829No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTATTAAAA5810.11230221473111256No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG5740.11094917599941241No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA5690.10998271976248371No Hit
GCACGGGACCATGCAAGACCTGCACGAGTCCTGCTCAAGGAACCTCTATG5670.10959613726771224No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG5650.10920955477294078No Hit
GTCTTTGGGTATACATTTGAACCCTAATAAAACCAAACGCTGGGGCTACT5610.10843638978339783No Hit
GTACAAAACCTTCGGACGGAAACTGCACTTGTATTCCCATCCCATCATCC5610.10843638978339783No Hit
CTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT5580.10785651604124064No Hit
CTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAGTGCC5540.10708335105169768No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCAC5540.10708335105169768No Hit
CTTTACCACAGGAACATATTGTATTAAAACTCAAGCAATGTTTTCGGAAA5470.10573031231999752No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT5470.10573031231999752No Hit
CCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGACCCGTAT5460.10553702107261179No Hit
CTCTTGGACTCTCAGCAATGTCAACGACCGACCTTGAGGCATACTTCAAA5440.10515043857784032No Hit
TTCCAGCCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCCCGTCTGT5390.10418398234091164No Hit
GTACAAAGATCATTAACCTGATCTCCTCCCCCAACTCCTCCCAGTCCTTA5380.1039906910935259No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA5260.10167119612489706No Hit
CTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCT5240.1012846136301256No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT5240.1012846136301256No Hit
CATGCATATAAAGGCATCAAGGCGGGATAGCCACATTGTGTAAAAGGAGC5230.10109132238273986No Hit
ATGTTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG5190.10031815739319691No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)