FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490464

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490464
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences428550
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCTAACCTTACCAAATATTTGCCATTGGACAAAGGCATTAAACCATAT14600.3406837008517093No Hit
ATATCGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCC14500.33835025084587567No Hit
CATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACT10790.25177925562944814No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA10680.24921246062303118No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9590.22377785555944466No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATCGCTGAGAGTCCAA9440.2202776805506942No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9430.22004433555011085No Hit
AACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATA9290.21677750554194375No Hit
CTTATATAGAATACCCGCCTTCCAAAGAGTATGTAAATAATGCCTAGTTT9010.21024384552560962No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8770.2046435655116089No Hit
CCCTAGCAGCCATGGAAATGAGGTGTATTTCCGAGAGAGGACAACAGAGT8700.20301015050752536No Hit
ATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCC8530.1990432854976082No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG8220.19180959047952398No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8110.189242795473107No Hit
CAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAAT8030.1873760354684401No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC7810.1822424454556061No Hit
CTATATAAGAGAGAAACTACACGCAGTGCCTCATTCTGTGGGTCACCATA7790.18177575545443939No Hit
CCTACTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7730.1803756854509392No Hit
ATTATGGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGG7620.17780889044452222No Hit
TCCAAAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA7480.17454206043635515No Hit
CTTATATAGAATGCCCGCCTTCCAAAGAGTATGTAAATAATGCCTAGTTT7400.17267530043168824No Hit
GTCTATTTACAGGCAATTTTCGAAAACATTGCTTGATTTTTAGTACAATA7270.16964181542410453No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA7230.16870843542177108No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCGATGTCAACGACCGACCT7030.16404153541010383No Hit
GGATAATATGGTTTAATGCCTTTGTCCAATGGCAAATATTTGGTAAGGTT7000.16334150040835377No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6920.16147474040368687No Hit
GACAAATACTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACT6890.16077470540193675No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG6800.15867460039668652No Hit
GGTCTATTTACAGGCAATTTTCGAAAACATTGCTTGATTTTTAGTACAAT6680.15587446038968614No Hit
CACAAATTGTTGACATCTATTAATAATGTCCTCCTGTAAATGAATGTGAG6510.15190759537976897No Hit
TCTCCTGGCTCAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTT6370.14864076537160192No Hit
ATACCTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA6310.14724069536810172No Hit
GCCATGAGAAACGGACTGAGGCCCACTCCCATAGGAATCTTGCGAAAGCC6300.14700735036751839No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCATTGGACAAAGGCATTAAAC6130.14304048535760122No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAA6130.14304048535760122No Hit
CATCGATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACT6120.14280714035701786No Hit
CACATATTGTTGACATCTATTAATAATGTCCTCCTGTAAATGAATGTGAG6010.14024034535060084No Hit
CATTTAAACCATAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5920.1381402403453506No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5900.13767355034418388No Hit
ATATAAGAGAGAAACTACACGCAGTGCCTCATTCTGTGGGTCACCATATT5770.13464006533660017No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG5730.13370668533426672No Hit
ATATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT5590.13043985532609964No Hit
ATGTAAGACCTTGGGCAAGGCCTGGTGGGCGTTCACGGTGGTTTCCATGC5570.12997316532493292No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA5530.12903978532259947No Hit
TTATTAATAGATGTCAACAATTTGTGGGCCCTCTTACAGTTAATGAAAAA5530.12903978532259947No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5430.12670633531676584No Hit
CAATTTGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAAT5370.12530626531326566No Hit
CTCCTACTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG5280.1232061603080154No Hit
GCCTTAGAGTCTCCGGAACATTGTTCTCCTCACCATACAGCACTCAGGCA5260.12273947030684867No Hit
GATTAAAATTAATTATGCCTGCTAGGTTCTATCCTAACCTTACCAAATAT5240.12227278030568195No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT5180.12087271030218177No Hit
GCACCAAACCTCGACAAGGCATGGGGACGAATCTTTCTGTTCCCAATCCT5180.12087271030218177No Hit
GCTTAGATTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACAT5160.12040602030101505No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA5150.12017267530043169No Hit
GGAAAGTATAGGCCCCTCACTCTGGGGTCTAGCAGAGCTTGGTGGAATGT5060.11807257029518142No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA5030.11737253529343133No Hit
CATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA4980.11620581029051452No Hit
GTTCAGGATAATATGGTTTAATGCCTTTGTCCAATGGCAAATATTTGGTA4980.11620581029051452No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG4970.11597246528993116No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT4970.11597246528993116No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4930.11503908528759771No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCTCTGACATACT4880.11387236028468091No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4880.11387236028468091No Hit
GAATACCCGCCTTCCAAAGAGTATGTAAATAATGCCTAGTTTTGAAGTAA4860.11340567028351417No Hit
GTTCAGAGCCAACTCAAACAATCCAGATTGGGACTTCAACCCCAACAAGG4830.1127056352817641No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4730.11037218527593046No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGGCGATGGCC4720.1101388402753471No Hit
CGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGGCAGATGAAGAAGG4720.1101388402753471No Hit
ATACTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA4710.10990549527476375No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAGAGAATTGTGGGTCTTTTGG4680.10920546027301364No Hit
CAGAATAGCTTGCCTGAGTGCTGTATGGTGAGGAGAACAATGTTCCGGAG4660.10873877027184693No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTTTTTCCTTCTATTCG4610.1075720452689301No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC4550.10617197526542994No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT4540.10593863026484658No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4490.10477190526192977No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG4490.10477190526192977No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC4450.10383852525959632No Hit
GTATTTGTCTGAGTTTTAGGCCCATATTAACATTGACATAGCTGACTACT4390.10243845525609613No Hit
GGAATCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG4380.10220511025551278No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG4310.10057169525142923No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4300.10033835025084588No Hit
CTCTTATATAGAATACCCGCCTTCCAAAGAGTATGTAAATAATGCCTAGT4290.10010500525026252No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)