FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490465

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490465
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences428550
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCC13710.31991599579978997No Hit
ATCCTAACCTTACCAAATATTTGCCATTGGACAAAGGCATTAAACCATAT12520.2921479407303698No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA10460.24407887061019717No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG10400.242678800606697No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9960.23241162058102904No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATCGCTGAGAGTCCAA9830.22937813557344536No Hit
CATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACT9570.22331116555827793No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG9570.22331116555827793No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9120.21281064053202658No Hit
CTTATATAGAATACCCGCCTTCCAAAGAGTATGTAAATAATGCCTAGTTT8980.20954381052385954No Hit
GTCTATTTACAGGCAATTTTCGAAAACATTGCTTGATTTTTAGTACAATA8900.20767705051919264No Hit
ATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCC8870.20697701551744252No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA8500.19834325049585813No Hit
CCCTAGCAGCCATGGAAATGAGGTGTATTTCCGAGAGAGGACAACAGAGT8320.1941430404853576No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC7740.18060903045152257No Hit
AACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATA7730.1803756854509392No Hit
CTTATATAGAATGCCCGCCTTCCAAAGAGTATGTAAATAATGCCTAGTTT7660.17874227044685567No Hit
CAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAAT7600.1773422004433555No Hit
GGATAATATGGTTTAATGCCTTTGTCCAATGGCAAATATTTGGTAAGGTT7490.1747754054369385No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7320.17080854042702134No Hit
CTATATAAGAGAGAAACTACACGCAGTGCCTCATTCTGTGGGTCACCATA7170.16730836541827093No Hit
GGTCTATTTACAGGCAATTTTCGAAAACATTGCTTGATTTTTAGTACAAT7140.16660833041652082No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG7050.16450822541127058No Hit
TCCAAAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA6990.16310815540777038No Hit
ATTATGGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGG6910.1612413954031035No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6900.16100805040252011No Hit
CCTACTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6860.16007467040018666No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG6710.15657449539143625No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCATTGGACAAAGGCATTAAAC6320.1474740403686851No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA6290.14677400536693502No Hit
GACAAATACTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACT6290.14677400536693502No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6260.1460739703651849No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAA6260.1460739703651849No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCGATGTCAACGACCGACCT6210.14490724536226812No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT6200.14467390036168476No Hit
ATACCTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA6120.14280714035701786No Hit
TCTCCTGGCTCAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTT6020.14047369035118423No Hit
ATATAAGAGAGAAACTACACGCAGTGCCTCATTCTGTGGGTCACCATATT5920.1381402403453506No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5790.1351067553377669No Hit
GCCATGAGAAACGGACTGAGGCCCACTCCCATAGGAATCTTGCGAAAGCC5730.13370668533426672No Hit
CTCCTACTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG5660.13207327033018318No Hit
CACAAATTGTTGACATCTATTAATAATGTCCTCCTGTAAATGAATGTGAG5560.12973982032434955No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA5500.12833975032084938No Hit
TTATTAATAGATGTCAACAATTTGTGGGCCCTCTTACAGTTAATGAAAAA5440.1269396803173492No Hit
ATATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT5380.12553961031384903No Hit
CAATTTGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAAT5360.1250729203126823No Hit
GTTCAGGATAATATGGTTTAATGCCTTTGTCCAATGGCAAATATTTGGTA5350.12483957531209894No Hit
CATCGATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACT5240.12227278030568195No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5230.12203943530509859No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA5200.1213394003033485No Hit
CACATATTGTTGACATCTATTAATAATGTCCTCCTGTAAATGAATGTGAG5120.1194726402986816No Hit
GCCTTAGAGTCTCCGGAACATTGTTCTCCTCACCATACAGCACTCAGGCA5110.11923929529809824No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5060.11807257029518142No Hit
GGAAAGTATAGGCCCCTCACTCTGGGGTCTAGCAGAGCTTGGTGGAATGT5050.11783922529459807No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCTCTGACATACT5040.11760588029401471No Hit
CTCTTATATAGAATACCCGCCTTCCAAAGAGTATGTAAATAATGCCTAGT5020.11713919029284797No Hit
GCTTAGATTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACAT4980.11620581029051452No Hit
CATTTAAACCATAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT4920.11480574028701436No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4920.11480574028701436No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG4910.11457239528643098No Hit
ATGTAAGACCTTGGGCAAGGCCTGGTGGGCGTTCACGGTGGTTTCCATGC4890.11410570528526426No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4880.11387236028468091No Hit
GATTAAAATTAATTATGCCTGCTAGGTTCTATCCTAACCTTACCAAATAT4850.11317232528293081No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGGCGATGGCC4820.11247229028118072No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG4730.11037218527593046No Hit
GTTCAGAGCCAACTCAAACAATCCAGATTGGGACTTCAACCCCAACAAGG4720.1101388402753471No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCTCCTCACCATACAG4710.10990549527476375No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG4690.109438805273597No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4690.109438805273597No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCATCTGCCGTTCCGGCCGACCACG4670.1089721152724303No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATATGGTTTAATGCC4660.10873877027184693No Hit
GTACTAAAAATCAAGCAATGTTTTCGAAAATTGCCTGTAAATAGACCTAT4650.10850542527126356No Hit
GAATACCCGCCTTCCAAAGAGTATGTAAATAATGCCTAGTTTTGAAGTAA4610.1075720452689301No Hit
TTTTAGTACAATATGTTCTTGCGGTAAAGTACCCCAACTTCCAATTACAT4570.10663866526659666No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC4550.10617197526542994No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT4540.10593863026484658No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC4520.10547194026367984No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG4510.10523859526309648No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG4420.10313849025784623No Hit
ATATGGTTTAATGCCTTTGTCCAATGGCAAATATTTGGTAAGGTTAGGAT4410.10290514525726287No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4410.10290514525726287No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAGAGAATTGTGGGTCTTTTGG4350.10150507525376268No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC4340.10127173025317933No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTTTTTCCTTCTATTCG4320.1008050402520126No Hit
GCACCAAACCTCGACAAGGCATGGGGACGAATCTTTCTGTTCCCAATCCT4320.1008050402520126No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC4310.10057169525142923No Hit
ATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTG4310.10057169525142923No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)