FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490467

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490467
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162548
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTATTTACAGGCAGTTTCCGAAAACATTGTTTGAGTTTTAATACAATA6500.39988188104436845No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAATTGGATCCTGCGCGGGACGTCC5370.3303639540320398No Hit
GTAAACAGACCTATTGATTGGAAAGTCTGTCAAAGAATTGTGGGTCTTTT5140.31621428747200825No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTGTCAACACTTCCGGA4640.2854541427762876No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA4510.27745650515540027No Hit
CCCTCATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATG4280.26330683859536874No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCAGACCTTTAACCT4080.2510027807170805No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACAGACT4070.2503875778231661No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA3430.21101459261264363No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA3340.20547776656741393No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG3330.2048625636734995No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC3160.1944041144769545No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA3110.19132810000738243No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG3100.190712897113468No Hit
TTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA3020.18579127396215273No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC3010.1851760710682383No Hit
ATGACAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGG3010.1851760710682383No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG2920.17963924502300857No Hit
GATTTAGACCTTCGTCTGCGAGGCGAGGGAGTTCTTCTTCTAGGGGACCT2870.17656323055343653No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG2810.17287201318995005No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG2800.17225681029603562No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG2790.17164160740212123No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA2790.17164160740212123No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC2710.16671998425080592No Hit
CCTTTTCATCTGCCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGC2690.16548957846297707No Hit
TTATCAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA2680.16487437556906268No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG2660.16364396978123386No Hit
CCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATG2600.15995275241774737No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT2570.15810714373600412No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT2500.15380072347860324No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC2420.14887910032728793No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA2410.14826389743337354No Hit
GGATGGGGCTTGGCCATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGC2410.14826389743337354No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG2410.14826389743337354No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG2400.1476486945394591No Hit
CTTCATCTGCCGTTCCAGCCGACCACGGGGCGCACCTCTCTTTACGCGGT2350.14457268006988705No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA2300.14149666560031499No Hit
GTAAATAGACCTATTGATTGGAAAGTCTGTCAAAGAATTGTGGGTCTTTT2280.14026625981248617No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG2280.14026625981248617No Hit
GTCCAAGAGTCCTCTTATGTAAGAGCTTGGGCAAGACCTGGTGGGCGTTC2260.13903585402465735No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC2250.13842065113074292No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG2240.13780544823682853No Hit
GCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA2230.1371902453429141No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC2220.13657504244899968No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA2160.1328838250855132No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC2140.13165341929768437No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA2130.13103821640376995No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG2120.13042301350985555No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA2120.13042301350985555No Hit
GGGTAAAGGTGCAGGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA2030.12488618746462583No Hit
GTTGTCAATGTGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG2000.1230405787828826No Hit
GATGACAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTG2000.1230405787828826No Hit
GATCCATACTGCGGAACTCCTAGCAGCTTGTTTTGCTCGCAGCCGGTCTG1990.12242537588896818No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT1990.12242537588896818No Hit
GCCATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGCTCCTCTGCCGAT1940.11934936141939612No Hit
GGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT1880.11565814405590964No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC1880.11565814405590964No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG1870.11504294116199522No Hit
ATAGAGGCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG1850.1138125353741664No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG1840.11319733248025199No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA1830.11258212958633758No Hit
GCAGACGAAGAAGGGGACGATAGAGGCCCAAACGGCCCCGAGACGGGTCG1830.11258212958633758No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA1830.11258212958633758No Hit
GCTTTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA1800.11073652090459435No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG1790.11012131801067992No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA1790.11012131801067992No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA1780.1095061151167655No Hit
GCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA1770.1088909122228511No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT1760.10827570932893668No Hit
GCCTATGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGC1750.10766050643502226No Hit
CTTTATATGCATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACT1750.10766050643502226No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCAC1730.10643010064719345No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG1730.10643010064719345No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT1710.10519969485936463No Hit
GTACAAAACCTTCGGACGGAAACTGCACTTGTATTCCCATCCCATCATCC1700.10458449196545021No Hit
ATGTTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG1690.10396928907153578No Hit
GTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGTAAGAGCTTGGG1690.10396928907153578No Hit
ATACTGCGGAACTCCTAGCAGCTTGTTTTGCTCGCAGCCGGTCTGGAGCA1680.10335408617762139No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT1660.10212368038979254No Hit
GACAAAGGACGTCCCGCGCAGGATCCAATTGGCAGCACACCCGAGCAGCC1650.10150847749587814No Hit
GTGTTGGGGTGAGTTAATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG1650.10150847749587814No Hit
GTGGGGGAAAGCCCTGCGAACCACTGAACAAATGGCACTAGTAAACTGAG1640.10089327460196373No Hit
CTCCTCGACACCGCCTCTGCTTTGTATCGGGAGGCCTTAGAGTCTCCGGA1640.10089327460196373No Hit
CTCTAAGGCCTCCCGATACAAAGCAGAGGCGGTGTCGAGGAGATCTCGAA1630.10027807170804931No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)