FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences355948
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG27000.758537763943048No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20430.5739602413835728No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG14200.3989346758515289No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT13120.3685931652938069No Hit
TTATTAATAGATGTCAACAATACGTGGGCCCTCTTACAGTTAATGAAAAA12280.344994212637801No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA12040.33825165473608504No Hit
ATATAAGACCTTGGGCAAGACCTGATGGGCGTTCACGGTGGTCTCCATGC10360.29105374942407314No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA10200.2865587108229292No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9780.27475923449492623No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9670.27166889545663975No Hit
CTTATATAAAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT9510.2671738568554957No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9350.2626788182543518No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT8770.2463843033252048No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG8740.2455414835874903No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT8310.23346106734691585No Hit
CTTTATACCTCTATAACCAATTTTCTTTTGTCTTTGGGTATACATCTGAA8100.22756132918291433No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA8080.22699944935777133No Hit
CTGTAAGAGGGCCCACGTATTGTTGACATCTATTAATAATGTCCTCCTGT7150.20087203748862192No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACATCCGACCT7120.20002921775090743No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA7060.19834357827547847No Hit
GTATACATCTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAAC6710.18851068133547597No Hit
TTCTTCTACTGTACCTTCCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC6660.18710598177261847No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6530.183453762909189No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG6520.1831728229966175No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC6500.1826109431714745No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC6440.18092530369604548No Hit
CTACTGTACCTTCCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC6380.1792396642206165No Hit
GTATACATGCATATAAAGGCTTCAAGGCAGGATAGCCACATTGTGTAAAA6000.16856394754289952No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTTTATATAAGAGAGAAACTACA5980.16800206771775653No Hit
GGATTAAAGGAAGGTACAGTAGAAGAATAAAGCCCAGTAAAATTTCCCAC5780.16238326946632656No Hit
CTCTTATATAAAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT5750.16154044972861203No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC5660.15901199051546855No Hit
CATCTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5640.15845011069032555No Hit
GGACCATATTGTACTAAAAATCAAGCAATGTTTTCGGAAACTGCCTGTAA5560.15620259138975356No Hit
CCCTAGCAGCCATGGGAATGAGGTGTATTTCCGTGAGAGGACAACCGAGT5380.1511456729634666No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA5150.1446840549743221No Hit
TGCAAGAACTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG5120.1438412352366076No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC5030.1413127760234641No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4990.1401890163731781No Hit
TCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTG4960.1393461966354636No Hit
GTGTAGTTTCTCTCTTATATAAAATGCCAGCCTTCCACAGAGTATGTAAA4930.1385033768977491No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC4930.1385033768977491No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGATGTTGACATTGCTGAGAGTCCAA4930.1385033768977491No Hit
GTTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC4910.1379414970726061No Hit
GCACGGGACCATGCAAGAACTGCACGATTCCTGCTCAAGGAACCTCTATG4910.1379414970726061No Hit
GGTATAAAGGGCCTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT4860.13653679750974862No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAGATGTATA4800.13485115803431963No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4760.1337273983840336No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT4730.1328845786463191No Hit
CTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTGTACCTTCC4710.13232269882117612No Hit
GGAACATATTGTACTAAAAATCAAGCAATGTTTTCGGAAACTGCCTGTAA4660.13091799925831865No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTC4550.12782766022003214No Hit
AGTCTGTACAACATCTTGAGGCCCTTTATACCTCTATAACCAATTTTCTT4530.12726578039488914No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC4490.12614202074460312No Hit
CCTTTATACCTCTATAACCAATTTTCTTTTGTCTTTGGGTATACATCTGA4480.12586108083203162No Hit
TTTACATACTCTGTGGAAGGCTGGCATTTTATATAAGAGAGAAACTACAC4460.12529920100688866No Hit
CCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAG4370.12277074179374516No Hit
TATTAATAGATGTCAACAATACGTGGGCCCTCTTACAGTTAATGAAAAAA4300.12080416240574465No Hit
CAACAATACGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATT4260.11968040275545866No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT4250.11939946284288715No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4240.11911852293031568No Hit
GCTTTATTCTTCTACTGTACCTTCCTTTAATCCTGAGTGGCAAACTCCCT4200.11799476328002967No Hit
GGATAAAGGCATTAAACCTTATTATCCTGAACATGCAGTTAATCATTACT4190.11771382336745817No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTAGTACAATA4080.11462348432917167No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4010.1126569049411712No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGGCCCTTTATACC3960.11125220537831369No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG3920.11012844572802769No Hit
CCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAAAACCTAGCAGG3860.1084428062525987No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG3840.10788092642745568No Hit
ATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGGAT3830.10759998651488419No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGAAGCCTTTATATGCATGTATA3830.10759998651488419No Hit
GAATATGGTGACCCACAAAATGAAGCGCTGCGTGTAGTTTCTCTCTTATA3820.10731904660231269No Hit
ATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGG3800.10675716677716969No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT3790.10647622686459819No Hit
ATATTTGCCCTTGGATAAAGGCATTAAACCTTATTATCCTGAACATGCAG3720.10450964747659772No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTAGTACAAT3710.1042287075640262No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTG3700.1039477676514547No Hit
CCTCCCGCTTACAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA3670.10310494791374021No Hit
ATTAATAGATGTCAACAATACGTGGGCCCTCTTACAGTTAATGAAAAAAG3630.1019811882634542No Hit
GTATAAAGAATTTGGAGCTTCAGTGGAGTTACTCTCTTTTTTGCCTTCTG3580.10057648870059671No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACAAAATGCC3560.10001460887545371No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)