FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences334418
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTGGTTTTGAAGTAATGATTAACTGCATGTTCAGG13350.39920099994617514No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTGGTTT11790.3525527932108917No Hit
GTATACATGCGTATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA11380.34029268759456727No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC11160.3337140943370273No Hit
CATGTATACAATCCAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC9300.2780950786141894No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT9120.27271259322165675No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8830.26404081120035405No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA8780.262545676369095No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC8400.2511826516515259No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8230.24609919322524507No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT7890.23593227637268327No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7560.22606438648637334No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG7480.2236721707563588No Hit
CCCTTAATTTCATGGGATATGTAATTGGATGTTGGGGTACTTTACCGCAA7310.21858871233007793No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7230.21619649660006338No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA7140.21350525390379707No Hit
ACCAAATATTTGCCCTTGGACAAAGGCATTAAACCATATTATCCTGAACA6690.2000490404224653No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTGGT6660.19915195952370984No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT6640.1985539055912062No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTAATTTTCAGTACAATA6630.1982548786249544No Hit
ATGTAATTGGATGTTGGGGTACTTTACCGCAAGAACATATTGTACTGAAA6590.19705877075994715No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC6390.1910782314349108No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6170.18449963817737083No Hit
ATATCTGGTAAGGTTGGGATAGAACCTAGCAGGCATAATTAATTTTAATC6150.18390158424486722No Hit
CTATATAAGAGAGAAACTACCCGCAGCGCCTCATTTTGTGGGTCACCATA6050.18091131458234905No Hit
CCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAATTTCATGGGATATG6030.1803132606498454No Hit
GGGTAAAGGTGCAGATATTGTTGATACAGAAAGGCCTTGTAAGTTGGCGA5990.17911715278483814No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT5950.17792104491983088No Hit
ATTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCAGT5850.17493077525731268No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT5840.1746317482910609No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAATTTCAT5610.1677541280672691No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCCATTCG5570.16655802020226182No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5240.1566901303159519No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC5210.15579304941719643No Hit
ATTCTATGTTGGGGTGAATTGATGAATCTGGCCACCTGGGTGGGAAGTAA5180.154895968518441No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG5090.15220472582217465No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATACGCATGTATA5050.15100861795716738No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5020.15011153705841193No Hit
GTACAATATGTTCTTGCGGTAAAGTACCCCAACATCCAATTACATATCCC5020.15011153705841193No Hit
CCCCTAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGAAGTATGCCTCA4960.14831737526090102No Hit
CTCTAAGGCCTCCCGATACAGAGCGGAGGCGGTGTCGAGGAGATCTCGAA4950.14801834829464922No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG4910.14682224042964195No Hit
GGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTAATTTTCAGTACAAT4900.14652321346339015No Hit
CTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCGAAAAC4890.1462241864971383No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4780.14293488986836833No Hit
GCACGGGACCATGCAGGACCTGCACGATTCCTGCTCAAGGAACCTCTATG4730.14143975503710923No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC4710.14084170110460562No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4690.14024364717210197No Hit
TCCCGATACAGAGCGGAGGCGGTGTCGAGGAGATCTCGAATGGAAGGAAA4640.1387485123408429No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG4630.13844948537459106No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG4600.1375524044758356No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4580.136954350543332No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT4500.13456213481331747No Hit
GGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT4480.13396408088081382No Hit
ATATAAGAGAGAAACTACCCGCAGCGCCTCATTTTGTGGGTCACCATATT4470.13366505391456202No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCCTGCATGGTCCCGTG4430.13246894604955475No Hit
ATTTACAGGCAGTTTTCGAAAACATTGCTTAATTTTCAGTACAATATGTT4420.13216991908330292No Hit
AATTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA4420.13216991908330292No Hit
GACCTTTAACCTAATCTCCTCCCCTAACTCCTCCCAGTCCTTAAACAAAC4420.13216991908330292No Hit
TCCTTTGTCTACGTCCCGTCAGCGCTGAATCCCGCGGACGACCCGTCTCG4340.1297777033532884No Hit
GTCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCTGACGGG4320.12917964942078475No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4300.12858159548828113No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG4240.12678743369077022No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA4230.12648840672451842No Hit
CAATAGGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTAATTTTCAGT4230.12648840672451842No Hit
TCCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCTAACTCCTCCCAGT4160.12439521796075571No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCATCTGCCGTTCCGGCCGACCACG4150.12409619099450389No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC4140.12379716402825205No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG4140.12379716402825205No Hit
GAGTATGTAAATAATGCCTGGTTTTGAAGTAATGATTAACTGCATGTTCA4140.12379716402825205No Hit
TCCACAGAGTATGTAAATAATGCCTGGTTTTGAAGTAATGATTAACTGCA4110.1229000831294966No Hit
TTATCAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA4090.12230202919699298No Hit
CAATTACATATCCCATGAAATTAAGGGAGTAGCCCCAACGTTTGGTTTTA4070.12170397526448935No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4050.12110592133198572No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC3980.119012732568223No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTACAGG3960.11841467863571936No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA3950.11811565166946755No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGG3930.11751759773696391No Hit
ATCGCCGCATGCGTGGAACCTTTGTGGCTCCTCTGCCGATCCATACTGCG3830.11452732807444574No Hit
GCCCAAAAGACCCACAATTCTCTGACATACTTTCCAATCAATAGGTCTAT3800.11363024717569031No Hit
CGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGGCAGATGAAGAAGG3800.11363024717569031No Hit
ATATTATCCTGAACATGCAGTTAATCATTACTTCAAAACCAGGCATTATT3760.11243413931068304No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC3700.11063997751317213No Hit
ATGTTAATATGGGTCTAAAAATCAGACAACTACTGTGGTTTCACATTTCC3700.11063997751317213No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAA3690.11034095054692032No Hit
GTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG3650.10914484268191305No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA3630.10854678874940941No Hit
GTCTACGTCCCGTCAGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG3600.10764970785065396No Hit
CTCACCAACCTCTTGTCCTCCAATTTGTCCTGGCTATCGCTGGATGTGTC3580.10705165391815033No Hit
CCCTTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTT3550.10615457301939488No Hit
GATCCATACTGCGGAACTCCTAGCAGCTTGTTTTGCTCGCAGCCGGTCTG3540.10585554605314308No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG3530.10555651908689126No Hit
GTTAATATGGGTCTAAAAATCAGACAACTACTGTGGTTTCACATTTCCTG3520.10525749212063945No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTT3500.1046594381881358No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG3460.10346333032312854No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA3440.10286527639062491No Hit
GTAGAGTCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCTG3430.10256624942437309No Hit
TAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT3430.10256624942437309No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC3430.10256624942437309No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAATCTCCTCC3430.10256624942437309No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACC3430.10256624942437309No Hit
TTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA3420.10226722245812127No Hit
CCTCTGCACGTCGCATGGAGACCACCGTGAACGCCCACCAGGTCTTGCCC3410.10196819549186946No Hit
CATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT3400.10166916852561764No Hit
CCTTTATACGCATGTATACAATCCAAGCAGGCTTTCACTTTCTCGCCAAC3380.101071114593114No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)