FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences334418
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTGGTTTTGAAGTAATGATTAACTGCATGTTCAGG14080.4210299684825578No Hit
GTATACATGCGTATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA12270.366906087590979No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTGGTTT11650.34836641568336635No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC10790.3226500965857101No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT10390.31068901793563747No Hit
CATGTATACAATCCAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC9570.2861688067029885No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8790.26284470333534676No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8330.24908946288776324No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA8300.24819238198900778No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7910.23653033030518691No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA7730.23114784491265425No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG7570.22636341345262517No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT7320.21888773929632974No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT7300.2182896853638261No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTAATTTTCAGTACAATA7260.21709357749881883No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7230.21619649660006338No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTGGT7160.21410330783630066No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC6860.20513249884874618No Hit
ATGTAATTGGATGTTGGGGTACTTTACCGCAAGAACATATTGTACTGAAA6780.20274028311873166No Hit
CCCTTAATTTCATGGGATATGTAATTGGATGTTGGGGTACTTTACCGCAA6690.2000490404224653No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCCATTCG6220.1859947730086299No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATACGCATGTATA6050.18091131458234905No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5980.1788181258185863No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC5930.17732299098732723No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5890.17612688312231997No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5700.17044537076353544No Hit
ATATCTGGTAAGGTTGGGATAGAACCTAGCAGGCATAATTAATTTTAATC5690.17014634379728363No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAATTTCAT5680.16984731683103182No Hit
CTATATAAGAGAGAAACTACCCGCAGCGCCTCATTTTGTGGGTCACCATA5660.16924926289852818No Hit
ATTCTATGTTGGGGTGAATTGATGAATCTGGCCACCTGGGTGGGAAGTAA5580.16685704716851366No Hit
ACCAAATATTTGCCCTTGGACAAAGGCATTAAACCATATTATCCTGAACA5530.16536191233725459No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA5520.16506288537100275No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5520.16506288537100275No Hit
CTCTAAGGCCTCCCGATACAGAGCGGAGGCGGTGTCGAGGAGATCTCGAA5480.1638667775059955No Hit
GGGTAAAGGTGCAGATATTGTTGATACAGAAAGGCCTTGTAAGTTGGCGA5440.16267066964098822No Hit
GTACAATATGTTCTTGCGGTAAAGTACCCCAACATCCAATTACATATCCC5400.16147456177598096No Hit
CCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAATTTCATGGGATATG5360.1602784539109737No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5260.15728818424845553No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT5170.15459694155218917No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT5060.1513076449234192No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG4990.14921445615965648No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCATCTGCCGTTCCGGCCGACCACG4970.14861640222715286No Hit
ATTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCAGT4970.14861640222715286No Hit
CCCCTAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGAAGTATGCCTCA4920.14712126739589376No Hit
GTCTACGTCCCGTCAGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG4910.14682224042964195No Hit
GGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTAATTTTCAGTACAAT4890.1462241864971383No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG4870.1456261325646347No Hit
TCCTTTGTCTACGTCCCGTCAGCGCTGAATCCCGCGGACGACCCGTCTCG4810.14383197076712378No Hit
CTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCGAAAAC4790.14323391683462014No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG4710.14084170110460562No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG4650.1390475393070947No Hit
ATATAAGAGAGAAACTACCCGCAGCGCCTCATTTTGTGGGTCACCATATT4460.1333660269483102No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG4440.13276797301580656No Hit
GGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT4400.1315718651507993No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTACAGG4370.13067478425204385No Hit
CAATAGGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTAATTTTCAGT4360.13037575728579204No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC4360.13037575728579204No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4350.1300767303195402No Hit
GCACGGGACCATGCAGGACCTGCACGATTCCTGCTCAAGGAACCTCTATG4300.12858159548828113No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA4270.12768451458952568No Hit
ATTTACAGGCAGTTTTCGAAAACATTGCTTAATTTTCAGTACAATATGTT4210.1258903527920148No Hit
GAGTATGTAAATAATGCCTGGTTTTGAAGTAATGATTAACTGCATGTTCA4180.12499327189325934No Hit
CCCTTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTT4110.1229000831294966No Hit
TCCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCTAACTCCTCCCAGT4110.1229000831294966No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4110.1229000831294966No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4110.1229000831294966No Hit
CAATTACATATCCCATGAAATTAAGGGAGTAGCCCCAACGTTTGGTTTTA4090.12230202919699298No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4090.12230202919699298No Hit
GACCTTTAACCTAATCTCCTCCCCTAACTCCTCCCAGTCCTTAAACAAAC4060.12140494829823753No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4060.12140494829823753No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC4030.12050786739948209No Hit
ATCGCCGCATGCGTGGAACCTTTGTGGCTCCTCTGCCGATCCATACTGCG3970.11871370560197118No Hit
CTGCCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAGACC3900.11662051683820848No Hit
TTATCAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA3890.11632148987195665No Hit
AATTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA3850.1151253820069494No Hit
CCTTTATACGCATGTATACAATCCAAGCAGGCTTTCACTTTCTCGCCAAC3850.1151253820069494No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA3840.11482635504069758No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC3830.11452732807444574No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC3810.11392927414194212No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG3750.11213511234443123No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCCTGCATGGTCCCGTG3740.1118360853781794No Hit
CTCACCAACCTCTTGTCCTCCAATTTGTCCTGGCTATCGCTGGATGTGTC3660.10944386964816487No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG3660.10944386964816487No Hit
TCCCGATACAGAGCGGAGGCGGTGTCGAGGAGATCTCGAATGGAAGGAAA3650.10914484268191305No Hit
TCCACAGAGTATGTAAATAATGCCTGGTTTTGAAGTAATGATTAACTGCA3650.10914484268191305No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAATCTCCTCC3630.10854678874940941No Hit
GCCCAAAAGACCCACAATTCTCTGACATACTTTCCAATCAATAGGTCTAT3620.10824776178315762No Hit
TTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA3580.10705165391815033No Hit
ATGTTAATATGGGTCTAAAAATCAGACAACTACTGTGGTTTCACATTTCC3580.10705165391815033No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG3550.10615457301939488No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC3530.10555651908689126No Hit
ATATTATCCTGAACATGCAGTTAATCATTACTTCAAAACCAGGCATTATT3520.10525749212063945No Hit
GTACAAAGACCTTTAACCTAATCTCCTCCCCTAACTCCTCCCAGTCCTTA3500.1046594381881358No Hit
CGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGGCAGATGAAGAAGG3490.104360411221884No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC3470.10376235728938035No Hit
ATATTGTACTGAAAATTAAGCAATGTTTTCGAAAACTGCCTGTAAATAGA3460.10346333032312854No Hit
GTCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCTGACGGG3450.10316430335687672No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA3420.10226722245812127No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA3420.10226722245812127No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT3410.10196819549186946No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACC3400.10166916852561764No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA3360.10047306066061037No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)