FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345766
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGTATGTTCCGG15780.4563780128757599No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC13340.3858100565122077No Hit
GTCTTTGAAGTACGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA13150.38031501072979995No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT10780.31177154491766107No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10420.30135987922467794No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAACCTTAT10330.29875696280143216No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9920.2868992324288681No Hit
TTATTAATAGATGTCAACAATATGTAGGCCCTCTTACCGTTAATGAAAAA8580.2481446990160976No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7940.22963507111746095No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA7920.22905664524562855No Hit
GTCCCGTACTGGTAGTTGATGTTCCTGGAAGCAGAGGACAAACGGGCAAC7810.22587530295055036No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC7740.22385081239913698No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT7180.2076548879878299No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAGAGACCCACAATTCTTTGGCATACT7070.20447354569275172No Hit
CTATTAATAATGTCCTCCTGCAAATGAATGTGAGGAAAGGAGGGAGTTTG6820.19724322229484678No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA6810.19695400935893062No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6730.19464030587160103No Hit
GTACCGTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAA6480.18740998247369609No Hit
GTACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG6470.1871207695377799No Hit
GTATACATGCATACAACGGCATTAAGGCAGGATAGCCACATTGTGTAAAA6410.1853854919222827No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG5720.16542979934406507No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC5690.16456216053631648No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA5530.15993475356165732No Hit
GTCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGGCATAA5420.15675341126657913No Hit
ACAATATGTAGGCCCTCTTACCGTTAATGAAAAAAGGAGATTAAAATTAA5410.15646419833066294No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5290.15299364309966856No Hit
GTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAA5140.1486554490609256No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT5040.14576331970176362No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGACCGGC4980.14402804208626643No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGATACTCCCTTAACTTCAT4950.14316040327851784No Hit
CTCCAAAAGACACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGA4950.14316040327851784No Hit
ATGTAGGCCCTCTTACCGTTAATGAAAAAAGGAGATTAAAATTAATTATG4930.14258197740668546No Hit
CTACGGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC4850.14026827391935587No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4760.13766535749611009No Hit
CTCTTACCGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG4660.13477322813694811No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAAC4650.13448401520103193No Hit
TCGCAGAAGATCTCAATCTCGGGAATCTCAATGTTAGTATCCCTTGGACT4640.1341948022651157No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGTTGGAATGTTGTGG4610.13332716345736711No Hit
GGTCTATTTACAGGAAGTTTTCGAAAACATTGCTTAATTTTCAGTACAAT4600.13303795052145093No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4600.13303795052145093No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC4580.13245952464961852No Hit
GGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAA4530.13101345997003755No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4480.12956739529045655No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG4370.1263860529953784No Hit
GATTTGCCTCTGGCCATTGATCCTTGTTGGGGTTGAAGTCCCAATCTGGA4340.1255184141876298No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG4300.124361562443965No Hit
ACCAAACGTTGGGGATACTCCCTTAACTTCATGGGATATGTAATTGGAAG4290.1240723495080488No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCGTTGTATGCATGTATA4280.12378313657213261No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG4280.12378313657213261No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4250.12291549776438401No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA4220.12204785895663542No Hit
GGGTAAAGGTTCAGATATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA4180.12089100721297062No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGTATGTTCC4110.11886651666155724No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC4070.11770966491789245No Hit
GTATGCATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA4070.11770966491789245No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT4060.11742045198197625No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4040.11684202611014385No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCGCTTATGT3980.11510674849464667No Hit
CCCTTAGACAAAGGCATTAAACCTTATTATCCGGAACATACAGTTAATCA3950.11423910968689807No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTGCAGG3880.1122146191354847No Hit
GACAGAAACTGCACTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAG3880.1122146191354847No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG3850.1113469803277361No Hit
CTTCAGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGAGGGAGTTCT3830.1107685544559037No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGTA3820.1104793415199875No Hit
GTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGAAACATAGAGGTTC3800.10990091564815511No Hit
ACATATTGTTGACATCTATTAATAATGTCCTCCTGCAAATGAATGTGAGG3800.10990091564815511No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA3780.10932248977632271No Hit
GGCCTACATATTGTTGACATCTATTAATAATGTCCTCCTGCAAATGAATG3750.10845485096857412No Hit
GATTAACTGTATGTTCCGGATAATAAGGTTTAATGCCTTTGTCTAAGGGC3730.10787642509674172No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC3710.10729799922490933No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG3710.10729799922490933No Hit
GTCCAAGAGTCCGCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC3700.10700878628899313No Hit
CATGCATACAACGGCATTAAGGCAGGATAGCCACATTGTGTAAAAGGGGC3690.10671957335307693No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG3610.10440586986574735No Hit
GCTCTATATCGGGAGGCCCTAGAGTCTCCGGAACATTGTTCACCTCACCA3590.10382744399391496No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGCGGCCG3580.10353823105799875No Hit
GTATCGGGAGGCCCTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG3520.10180295344250156No Hit
CTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAGATTCCTATGGGAG3520.10180295344250156No Hit
ATCCCAGAGTGAGGGGCCTATACTTTCCTGCTGGTGGCTCCAGTTCCGGA3510.10151374050658539No Hit
GTCTTACATAAGCGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT3470.10035688876292058No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)