FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345766
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGTATGTTCCGG17080.4939756945448656No Hit
GTCTTTGAAGTACGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA12800.370192557972733No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC12000.34705552309943716No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT10640.3077225638148343No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10450.30222751803242653No Hit
TTATTAATAGATGTCAACAATATGTAGGCCCTCTTACCGTTAATGAAAAA10350.2993353886732646No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAACCTTAT9580.27706599260771736No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9370.2709925209534772No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA8710.2519044671830082No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8030.2322379875407067No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC7870.22761058056604755No Hit
GTCCCGTACTGGTAGTTGATGTTCCTGGAAGCAGAGGACAAACGGGCAAC7370.21314993377023767No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA7360.21286072083432148No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT7240.2093901656033271No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAGAGACCCACAATTCTTTGGCATACT7160.2070764621159975No Hit
CTATTAATAATGTCCTCCTGCAAATGAATGTGAGGAAAGGAGGGAGTTTG6600.19088053770469046No Hit
GTACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG6590.19059132476877427No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT6470.1871207695377799No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC6220.17989044613987495No Hit
GTACCGTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAA6140.17757674265254536No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6010.1738169744856348No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG5910.1709248451264728No Hit
ACAATATGTAGGCCCTCTTACCGTTAATGAAAAAAGGAGATTAAAATTAA5860.16947878044689182No Hit
GTATACATGCATACAACGGCATTAAGGCAGGATAGCCACATTGTGTAAAA5700.16485137347223267No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT5610.16224845704898688No Hit
GTCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGGCATAA5560.1608023923694059No Hit
CTCCAAAAGACACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGA5560.1608023923694059No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5440.15733183713841153No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAAC5380.15559655952291435No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG5090.1472093843813446No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGTTGGAATGTTGTGG5000.1446064679580988No Hit
ATGTAGGCCCTCTTACCGTTAATGAAAAAAGGAGATTAAAATTAATTATG5000.1446064679580988No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4980.14402804208626643No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGATACTCCCTTAACTTCAT4900.14171433859893687No Hit
GGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAA4700.1359300798806129No Hit
GGTCTATTTACAGGAAGTTTTCGAAAACATTGCTTAATTTTCAGTACAAT4670.1350624410728643No Hit
GTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAA4670.1350624410728643No Hit
CTACGGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC4600.13303795052145093No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCGTTGTATGCATGTATA4560.13188109877778614No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA4550.13159188584186993No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG4540.13130267290595374No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC4530.13101345997003755No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGTATGTTCC4510.13043503409820514No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4480.12956739529045655No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4470.12927818235454036No Hit
TCGCAGAAGATCTCAATCTCGGGAATCTCAATGTTAGTATCCCTTGGACT4440.12841054354679177No Hit
GTATGCATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA4390.12696447886721077No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGCGGCCG4240.12262628482846781No Hit
CTCTTACCGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG4200.12146943308480301No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG4150.12002336840522203No Hit
TATTAATAGATGTCAACAATATGTAGGCCCTCTTACCGTTAATGAAAAAA4120.11915572959747343No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC4080.11799887785380865No Hit
GTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGAAACATAGAGGTTC4060.11742045198197625No Hit
ACATATTGTTGACATCTATTAATAATGTCCTCCTGCAAATGAATGTGAGG4050.11713123904606006No Hit
ACCAAACGTTGGGGATACTCCCTTAACTTCATGGGATATGTAATTGGAAG4000.11568517436647907No Hit
ATTTACAGGAAGTTTTCGAAAACATTGCTTAATTTTCAGTACAATATGTT3990.11539596143056287No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG3960.11452832262281427No Hit
ATATTGTACTGAAAATTAAGCAATGTTTTCGAAAACTTCCTGTAAATAGA3960.11452832262281427No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG3950.11423910968689807No Hit
CTCCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG3940.11394989675098188No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCCGGTCTCTGCCAAGTGTTTGCTG3930.11366068381506568No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGTA3930.11366068381506568No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA3870.11192540619956849No Hit
GGGTAAAGGTTCAGATATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA3870.11192540619956849No Hit
GATTAACTGTATGTTCCGGATAATAAGGTTTAATGCCTTTGTCTAAGGGC3870.11192540619956849No Hit
GTTTTGAAGTAATGATTAACTGTATGTTCCGGATAATAAGGTTTAATGCC3860.11163619326365229No Hit
GATTTGCCTCTGGCCATTGATCCTTGTTGGGGTTGAAGTCCCAATCTGGA3810.1101901285840713No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG3800.10990091564815511No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT3760.10874406390449032No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTGCAGG3740.10816563803265793No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA3730.10787642509674172No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC3720.10758721216082552No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGACCGGC3710.10729799922490933No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG3690.10671957335307693No Hit
GTATCGGGAGGCCCTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG3670.10614114748124454No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT3650.10556272160941214No Hit
CTATCGTCCCCTTCTGCATCTGCCGTTCCGGCCGACCACGGGGCGCACCT3640.10527350867349594No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA3630.10498429573757977No Hit
GACAGAAACTGCACTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAG3620.10469508280166355No Hit
AAATAGACCTGTTGATTGGAAAGTATGCCAAAGAATTGTGGGTCTCTTGG3580.10353823105799875No Hit
GGCCTACATATTGTTGACATCTATTAATAATGTCCTCCTGCAAATGAATG3570.10324901812208258No Hit
GTCTTACATAAGCGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT3570.10324901812208258No Hit
CTTCAGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGAGGGAGTTCT3510.10151374050658539No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCGCTTATGT3500.10122452757066917No Hit
CTTCTGCATCTGCCGTTCCGGCCGACCACGGGGCGCACCTCTCTTTACGC3470.10035688876292058No Hit
GTTAAGGGAGTATCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATAC3470.10035688876292058No Hit
GCTCTATATCGGGAGGCCCTAGAGTCTCCGGAACATTGTTCACCTCACCA3460.10006767582700438No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)