FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences308577
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC20380.6604510381525519No Hit
CCTCCTGGATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA15540.5036020182968918No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACTGAAAGTCCAA14290.46309349044160775No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG13150.42614971303758864No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG12860.4167517345751628No Hit
GTATTTGGTAACAGTGGGATAAAATCTAGCGGGCATAATTAATTTTAATC10690.34642893021838955No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAAGCCCCCATG10390.3367068835331214No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC10270.3328180648590141No Hit
ACTCACAACTGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT9330.30235565191184044No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA8630.2796708763128814No Hit
GCTCAGAATACTGCCTCTGCCATATCGTCAACCTTCTCGAAGACTGGGGA8530.2764301940844587No Hit
ATGTAAGACCTTGGGCAGGTTCCGGTGGGCGTTCACGGTGGTCTCCATGC8020.2599027147195027No Hit
CAATTACATATCCCATGAAATTAAGGGAATATCCCCATCTTTTTGTTTTG7930.2569861007139223No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCATCAAACTCTTCAAG7840.25406948670834184No Hit
ATACCATATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA7520.2436993035773891No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAATTTCAT7370.238838280234755No Hit
ATGTCATTGGGAGTTGGGGATCCTTGCCACAGGACCATATTGTACAAAAA7180.23268098400075185No Hit
GCACCGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG7120.23073657466369818No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC6950.22522741487537956No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAA6570.2129128224073732No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATGTTTCAGCTCACCATACGG6220.20157043460789364No Hit
GTGTAAAAGGGGCAGCGAACCCCAAAAGACCCACAATCCGTTGACATACT6180.20027416171652457No Hit
ATCCAGAGCATGTAGTTAATCATTACTTTCAGACGAGACATTATTTACAT6100.1976816159337864No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC6050.19606127481957503No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT5990.19411686548252138No Hit
GGATAATAGGGTTTGATTCCTTTATCTAAGGGCAAGTATTTGGTAACAGT5890.19087618325409866No Hit
CCATACGGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATC5740.18601515991146456No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG5740.18601515991146456No Hit
ATATGGTCCTGTGGCAAGGATCCCCAACTCCCAATTACATATCCCATGAA5740.18601515991146456No Hit
ATATGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC5730.18569109168862227No Hit
ATGTAATTGGGAGTTGGGGATCCTTGCCACAGGACCATATTGTACAAAAA5670.18374668235156866No Hit
CCTCTAAGAGACACGCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT5490.17791345434040776No Hit
GGCTTTTACTTTCTCGCCAACTTACAAGGCCTTTCTGAGTAAACAGTATC5430.17596904500335409No Hit
GTCCGGTGCTGGTGGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC5430.17596904500335409No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTAC5420.17564497678051183No Hit
CCCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGACGACACATAGCGCC5420.17564497678051183No Hit
GTGTCGTCTCTCTTTTATATAAGATACCCGCCTTCCAAAGGGTATGTAAA5340.17305243099777365No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAAC5280.17110802166072No Hit
CCCTTATCTCCACCTCTAAGAGACACGCATCCTCAGGCCATGCAGTGGAA5260.17045988521503547No Hit
ATATAAAAGAGAGACGACACATAGCGCCTCATTTTGCGGGTCACCATATT5190.16819140765513954No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA5170.167543271209455No Hit
GGGTTCACCCCTCCCCATGGGGGCTTGTTGGGGTGGAGCCCTCAGGCTCA5160.16721920298661275No Hit
ATTCTGAGCAGGGCTTACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT5120.16592293009524364No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATATGGTATTGGGGGC5020.16268224786682092No Hit
GAGGCAGATGATAAAACGCCGCAGATACATCCAGCGATAACCAGGACAAA5010.16235817964397867No Hit
GAACAAATGGCACTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCAC4960.16073783852976728No Hit
ATCTTCCTCTGCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTTCT4940.16008970208408274No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4900.15879342919271366No Hit
GGACCATATTGTACAAAAAATCAAAGTATGTTTTCGAAAACTTCCTGTAA4760.15425647407292184No Hit
GGCCATCAGCGCGTGCGCGGAACCTTTGTGTCTCCGTTGCCGATCCACAC4750.15393240585007956No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG4750.15393240585007956No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGGATATAGACCACAAAATGCC4740.15360833762723727No Hit
ATCTAAGGGCAAGTATTTGGTAACAGTGGGATAAAATCTAGCGGGCATAA4710.15263613295871048No Hit
CTATTATCCAGAGCATGTAGTTAATCATTACTTTCAGACGAGACATTATT4620.14971951895313002No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG4570.14809917783891866No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATCATT4440.14388629094196909No Hit
CAAATCTTTCTGTCCCCAATCCACTGGGATTCTTCCCCGATCATCAGTTG4400.1425900180506No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAACAG4390.14226594982775773No Hit
GCCTGAGGATGCGTGTCTCTTAGAGGTGGAGATAAGGGAGTAGGCTGCCT4350.14096967693638865No Hit
CTTTTACACAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA4330.1403215404907041No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT4290.139025267599335No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGGTCCCCGAGATTGAGATCT4290.139025267599335No Hit
GGTCTATGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCTTGGC4270.13837713115365047No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACCACC4230.13708085826228136No Hit
GTACAGGGTCCCCAGTCTTCGAGAAGGTTGACGATATGGCAGAGGCAGTA4210.13643272181659685No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC4170.13513644892522775No Hit
GTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTATGCCAAG4150.13448831247954318No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATAAGTTT4110.1331920395881741No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG4090.13254390314248957No Hit
GCCTTAGAGTCTCCGGAACATGTTTCAGCTCACCATACGGCACTCAGGCA4090.13254390314248957No Hit
TATCCACATTGTGTAAAAGGGGCAGCGAACCCCAAAAGACCCACAATCCG4060.13157169847396274No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAGGTTCCGGTGGGCGTTC4060.13157169847396274No Hit
GGATATCCACATTGTGTAAAAGGGGCAGCGAACCCCAAAAGACCCACAAT4040.13092356202827818No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCGGTG4000.1296272891369091No Hit
GTACAGACTTGGCCCCCAATACCATATCATCCATATAACTGAAAGCCAGA3970.12865508446838228No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA3960.12833101624554No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG3950.12800694802269774No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGTACGG3950.12800694802269774No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG3930.1273588115770132No Hit
GAGTAAACAGTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTC3920.12703474335417092No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG3900.12638660690848638No Hit
CAATGACATATCCCATGAAATTAAGGGAATATCCCCATCTTTTTGTTTTG3890.1260625386856441No Hit
TCCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA3860.12509033401711728No Hit
GGTGTAAGCAATTTGTGGGACCCCTAACAGTAAATGAAAACAGGAGATTA3840.12444219757143274No Hit
CAGTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT3840.12444219757143274No Hit
GCCCGTTTGTCCTCTAATTCCAGGATCATCAACCACCAGCACCGGACCAT3820.1237940611257482No Hit
CCTCAAGGTCGGTCGTTGACATTACTGAAAGTCCAAGAGTCCTCTTATGT3800.12314592468006365No Hit
GTCCTCCAATTTGTCCTGGTTATCGCTGGATGTATCTGCGGCGTTTTATC3780.12249778823437911No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG3740.12120151534301No Hit
TCCAAAGGGTATGTAAATAATGTCTCGTCTGAAAGTAATGATTAACTACA3730.12087744712016774No Hit
CTCTAATTCCAGGATCATCAACCACCAGCACCGGACCATGCAGAACCTGC3720.12055337889732548No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT3710.12022931067448318No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA3690.11958117422879865No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC3670.1189330377831141No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG3670.1189330377831141No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA3640.11796083311458729No Hit
GTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATAAGTTTTGC3640.11796083311458729No Hit
GTGTGGATCGGCAACGGAGACACAAAGGTTCCGCGCACGCGCTGATGGCC3630.11763676489174502No Hit
GGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA3630.11763676489174502No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG3630.11763676489174502No Hit
ATTTATGCCTACAGCCTCCTAATACAAAGATCATTAACCTAGTCTCCTCC3620.11731269666890275No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA3620.11731269666890275No Hit
GTATACATCATTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC3600.1166645602232182No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG3590.11634049200037593No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCCTCTATTCG3560.11536828733184909No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTATGCCAAGTGTTTGCTG3550.11504421910900682No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA3530.11439608266332228No Hit
GTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG3510.11374794621763773No Hit
CAGTAAGCCCTGCTCAGAATACTGCCTCTGCCATATCGTCAACCTTCTCG3490.11309980977195319No Hit
GTCGATACGGCAGACGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGG3490.11309980977195319No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGGGTATGTAAATAATGTCTCGT3470.11245167332626865No Hit
CTGTTAGGGGTCCCACAAATTGCTTACACCTATCAACAATGTCCTCCTGC3440.11147946865774183No Hit
CAATAGGCCTGTTTACAGGAAGTTTTCGAAAACATACTTTGATTTTTTGT3440.11147946865774183No Hit
CCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGC3410.11050726398921501No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACCT3410.11050726398921501No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGT3390.10985912754353047No Hit
GTCTATATCCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATAT3350.10856285465216137No Hit
GAATCAGAGGACAAACGGGCAACATACCTTGATAGTCCAGAAGAACCAAC3340.1082387864293191No Hit
GCTCTGGATAATAGGGTTTGATTCCTTTATCTAAGGGCAAGTATTTGGTA3310.10726658176079228No Hit
CAATCCGTTGACATACTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTT3300.10694251353795No Hit
CTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACA3250.10532217242373865No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTATGCCAAGTG3250.10532217242373865No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCGTGCGCGGAACCTTTGTGTC3240.10499810420089638No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCTAAACAGGCTTTTACTTT3190.10337776308668502No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAAGGGGCAGCG3190.10337776308668502No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGTGGATTGGGGACAGAAAGAT3190.10337776308668502No Hit
GCCTACTCCCTTATCTCCACCTCTAAGAGACACGCATCCTCAGGCCATGC3170.10272962664100045No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG3140.10175742197247364No Hit
TAAATGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCATATCATC3140.10175742197247364No Hit
CCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT3100.10046114908110455No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)