FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences308577
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTGGATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA23020.7460050489829119No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC17860.5787858459962991No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG12970.4203164850264278No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG12090.39179848141630774No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACTGAAAGTCCAA11650.3775394796112478No Hit
GCTCAGAATACTGCCTCTGCCATATCGTCAACCTTCTCGAAGACTGGGGA10800.3499936806696546No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC9890.32050347239100774No Hit
GTATTTGGTAACAGTGGGATAAAATCTAGCGGGCATAATTAATTTTAATC9540.3091610845915282No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAAGCCCCCATG9260.30008717435194454No Hit
ACTCACAACTGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT8660.2806430809814082No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAATTTCAT7840.25406948670834184No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATGTTTCAGCTCACCATACGG7840.25406948670834184No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCATCAAACTCTTCAAG7450.2414308260174932No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA7120.23073657466369818No Hit
ATGTCATTGGGAGTTGGGGATCCTTGCCACAGGACCATATTGTACAAAAA7100.23008843821801364No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAA7050.22846809710380228No Hit
ATACCATATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA6990.22652368776674867No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC6980.22619961954390638No Hit
CAATTACATATCCCATGAAATTAAGGGAATATCCCCATCTTTTTGTTTTG6680.2164775728586382No Hit
CAAATCTTTCTGTCCCCAATCCACTGGGATTCTTCCCCGATCATCAGTTG6530.21161654951600412No Hit
ATGTAATTGGGAGTTGGGGATCCTTGCCACAGGACCATATTGTACAAAAA6060.1963853430424173No Hit
GCACCGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG5950.19282059259115228No Hit
GTGTAAAAGGGGCAGCGAACCCCAAAAGACCCACAATCCGTTGACATACT5950.19282059259115228No Hit
ATATGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC5940.19249652436831002No Hit
ATATGGTCCTGTGGCAAGGATCCCCAACTCCCAATTACATATCCCATGAA5680.1840707505744109No Hit
GTCCGGTGCTGGTGGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC5660.18342261412872637No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5640.18277447768304184No Hit
GGATAATAGGGTTTGATTCCTTTATCTAAGGGCAAGTATTTGGTAACAGT5530.17920972723177683No Hit
CCATACGGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATC5510.1785615907860923No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT5460.17694124967188093No Hit
GGCCATCAGCGCGTGCGCGGAACCTTTGTGTCTCCGTTGCCGATCCACAC5430.17596904500335409No Hit
ATATAAAAGAGAGACGACACATAGCGCCTCATTTTGCGGGTCACCATATT5360.1737005674434582No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGGATATAGACCACAAAATGCC5300.17175615810640457No Hit
ATCCAGAGCATGTAGTTAATCATTACTTTCAGACGAGACATTATTTACAT5290.1714320898835623No Hit
CCTCTAAGAGACACGCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT5260.17045988521503547No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAAC5240.1698117487693509No Hit
ATGTAAGACCTTGGGCAGGTTCCGGTGGGCGTTCACGGTGGTCTCCATGC5210.1688395441008241No Hit
GGGTTCACCCCTCCCCATGGGGGCTTGTTGGGGTGGAGCCCTCAGGCTCA5180.16786733943229729No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATATGGTATTGGGGGC5160.16721920298661275No Hit
CCCTTATCTCCACCTCTAAGAGACACGCATCCTCAGGCCATGCAGTGGAA5130.16624699831808593No Hit
GGCTTTTACTTTCTCGCCAACTTACAAGGCCTTTCTGAGTAAACAGTATC5110.1655988618724014No Hit
GAACAAATGGCACTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCAC5070.16430258898103228No Hit
GGACCATATTGTACAAAAAATCAAAGTATGTTTTCGAAAACTTCCTGTAA5060.16397852075819003No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG5050.16365445253534774No Hit
CCCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGACGACACATAGCGCC4980.16138597497545182No Hit
GTATACATCATTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC4800.15555274696429094No Hit
ATCTTCCTCTGCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTTCT4790.15522867874144863No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA4780.15490461051860638No Hit
ATTCTGAGCAGGGCTTACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT4660.15101579184449912No Hit
GAGGCAGATGATAAAACGCCGCAGATACATCCAGCGATAACCAGGACAAA4630.15004358717597227No Hit
GGTCTATGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCTTGGC4520.1464788367247073No Hit
CTATTATCCAGAGCATGTAGTTAATCATTACTTTCAGACGAGACATTATT4510.146154768501865No Hit
GTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTATGCCAAG4500.14583070027902273No Hit
GCCTGAGGATGCGTGTCTCTTAGAGGTGGAGATAAGGGAGTAGGCTGCCT4490.14550663205618047No Hit
TCCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA4470.14485849561049594No Hit
GTGTCGTCTCTCTTTTATATAAGATACCCGCCTTCCAAAGGGTATGTAAA4460.14453442738765365No Hit
CCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGC4450.14421035916481137No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAACAG4380.14194188160491547No Hit
GTACAGACTTGGCCCCCAATACCATATCATCCATATAACTGAAAGCCAGA4300.1393493358221773No Hit
ATCTAAGGGCAAGTATTTGGTAACAGTGGGATAAAATCTAGCGGGCATAA4140.13416424425670093No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC4140.13416424425670093No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4080.13221983491964728No Hit
CTTTTACACAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA4080.13221983491964728No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCGGTG4040.13092356202827818No Hit
GAGTAAACAGTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTC4030.13059949380543592No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTAC4000.1296272891369091No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG4000.1296272891369091No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATAAGTTT4000.1296272891369091No Hit
GTACAGGGTCCCCAGTCTTCGAGAAGGTTGACGATATGGCAGAGGCAGTA3910.12671067513132864No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC3900.12638660690848638No Hit
TCCAAAGGGTATGTAAATAATGTCTCGTCTGAAAGTAATGATTAACTACA3850.12476626579427501No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG3810.12346999290290592No Hit
GGTGTAAGCAATTTGTGGGACCCCTAACAGTAAATGAAAACAGGAGATTA3750.12152558356585227No Hit
GAATACTGCCTCTGCCATATCGTCAACCTTCTCGAAGACTGGGGACCCTG3730.12087744712016774No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG3710.12022931067448318No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT3670.1189330377831141No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG3670.1189330377831141No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG3640.11796083311458729No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACCACC3640.11796083311458729No Hit
GCCTTAGAGTCTCCGGAACATGTTTCAGCTCACCATACGGCACTCAGGCA3640.11796083311458729No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGT3620.11731269666890275No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGGTCCCCGAGATTGAGATCT3620.11731269666890275No Hit
GGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA3600.1166645602232182No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGGGTATGTAAATAATGTCTCGT3570.11569235555469139No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC3570.11569235555469139No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA3560.11536828733184909No Hit
TATCCACATTGTGTAAAAGGGGCAGCGAACCCCAAAAGACCCACAATCCG3560.11536828733184909No Hit
CTCTAATTCCAGGATCATCAACCACCAGCACCGGACCATGCAGAACCTGC3550.11504421910900682No Hit
GGATATCCACATTGTGTAAAAGGGGCAGCGAACCCCAAAAGACCCACAAT3530.11439608266332228No Hit
CAATCCGTTGACATACTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTT3480.11277574154911092No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT3470.11245167332626865No Hit
CTGTTAGGGGTCCCACAAATTGCTTACACCTATCAACAATGTCCTCCTGC3370.1092109910978459No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG3340.1082387864293191No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGTGGATTGGGGACAGAAAGAT3340.1082387864293191No Hit
GTATGTCAACGGATTGTGGGTCTTTTGGGGTTCGCTGCCCCTTTTACACA3330.10791471820647683No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTATGCCAAGTG3320.10759064998363455No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTATGCCAAGTGTTTGCTG3300.10694251353795No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA3300.10694251353795No Hit
GTCCTCCAATTTGTCCTGGTTATCGCTGGATGTATCTGCGGCGTTTTATC3290.10661844531510774No Hit
GCCTACTCCCTTATCTCCACCTCTAAGAGACACGCATCCTCAGGCCATGC3290.10661844531510774No Hit
CAGTAAGCCCTGCTCAGAATACTGCCTCTGCCATATCGTCAACCTTCTCG3280.10629437709226547No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAGGTTCCGGTGGGCGTTC3280.10629437709226547No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATCATT3270.10597030886942319No Hit
CAATAGGCCTGTTTACAGGAAGTTTTCGAAAACATACTTTGATTTTTTGT3260.10564624064658092No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG3260.10564624064658092No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG3250.10532217242373865No Hit
GTCGATACGGCAGACGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGG3240.10499810420089638No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG3220.10434996775521181No Hit
ATCTTATATAAAAGAGAGACGACACATAGCGCCTCATTTTGCGGGTCACC3200.10370183130952729No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG3200.10370183130952729No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA3190.10337776308668502No Hit
GTCTATATCCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATAT3180.10305369486384275No Hit
GCTTACACCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAA3180.10305369486384275No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCTAAACAGGCTTTTACTTT3170.10272962664100045No Hit
GCCCGTTTGTCCTCTAATTCCAGGATCATCAACCACCAGCACCGGACCAT3170.10272962664100045No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA3150.10208149019531591No Hit
AGATAAGGGAGTAGGCTGCCTTCCTGACTGCCGATTGGTGGAGGCAGGAG3120.10110928552678909No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCCTCTATTCG3110.10078521730394682No Hit
GTGTGGATCGGCAACGGAGACACAAAGGTTCCGCGCACGCGCTGATGGCC3100.10046114908110455No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)