FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490477

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490477
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences348122
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCCGG12160.34930283061685274No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG11670.3352273053699565No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCCGAGAGTCCAA10970.31511941216010475No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC10630.30535272117246254No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT9760.28036148246878967No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA9360.2688712577774458No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT8840.25393396567869886No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT8810.25307219882684806No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8760.25163592074043006No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG8580.24646531962932536No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA8000.2298044938268768No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG7660.22003780283923452No Hit
GTATACATGCATATAAGGGCATTAAGGCAGGATAGCCACATTGTGTAAAA7600.21831426913553298No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG7380.21199464555529385No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7280.20912208938245785No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT6840.19648284222197965No Hit
GTGTAAAAGGGGCAGCAAAGCCCAGAAGACCCACAATTCTTTGACAGACT6650.19102498549359131No Hit
CATGTATACAATCTAAGCAGGCTTTTACTTTCTCGCCAACTTACAAGGCC6630.19045047425902412No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG6350.18240731697508344No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC6340.18212006135779985No Hit
CCCCTATCTTATCCACACTTCCGGAAACTACTGTTGTTAGACGACGAGGC5840.16775728049362007No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5720.1643102130862169No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA5700.1637357018516497No Hit
ATCTTATCCACACTTCCGGAAACTACTGTTGTTAGACGACGAGGCAGGTC5690.1634484462343661No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT5660.16258667938251534No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG5650.16229942376523174No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTACTTCCGAGAGAGGACAACAGAGT5540.15913961197511217No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5340.15339449962944027No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC5260.15109645469117147No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5190.1490856653701863No Hit
GATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTG5190.1490856653701863No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4990.1433405530245144No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC4980.1430532974072308No Hit
AGATAGGGGCATTTGGTGGTCTGTAAGCGGGAGGAGTGCGAATCCACACT4980.1430532974072308No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCCACACTTCCGGA4900.14075525246896203No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC4890.14046799685167843No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC4870.13989348561711124No Hit
ATCCTAACAGTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT4840.13903171876526044No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG4670.1341483732714393No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC4470.1284032609257674No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGGTGGCC4460.1281160053084838No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4430.12725423845663303No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTACAGG4330.12438168228379705No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC4280.12294540419737908No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT4250.12208363734552828No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT4190.12036010364182671No Hit
ATGTTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCACATTTCC4160.11949833678997591No Hit
ACTAATTCCCTGGATGCTGGATCTTCCAAATTACCTCCCACCCAGGTGGC4120.11834931432084154No Hit
ATATCTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA4120.11834931432084154No Hit
ATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTG4110.11806205870355795No Hit
CTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGGAGTTCCACTGCATG4080.11720029185170716No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG4030.11576401376528918No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG3990.1146149912961548No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT3950.11346596882702041No Hit
GGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA3930.11289145759245323No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTGGGGTTCAAATGTATA3930.11289145759245323No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC3870.11116792388875166No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAGGTAATTTGG3860.11088066827146806No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA3850.11059341265418446No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA3850.11059341265418446No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT3710.10657183401221412No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG3710.10657183401221412No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA3700.10628457839493052No Hit
ATGTGGTATTGGGGGCGAAGTCTGTACAACATCTTGAGTCCCTTTTTACC3700.10628457839493052No Hit
GTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCC3670.10542281154307973No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA3660.10513555592579613No Hit
GTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTATTATCCGGAA3660.10513555592579613No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG3650.10484830030851253No Hit
GGATGGGGCTTGGCCATAGGCCACCAGCGCATGCGTGGAACCTTTGTGGC3630.10427378907394536No Hit
GTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGGAT3630.10427378907394536No Hit
GCTTTAGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTTCTGTGG3620.10398653345666176No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG3600.10341202222209456No Hit
GTTCTATCCTAACAGTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC3600.10341202222209456No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC3600.10341202222209456No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG3580.10283751098752736No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA3570.10255025537024376No Hit
ATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG3560.10226299975296016No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG3530.10140123290110939No Hit
CCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTGCCACTCAGGATTAAAG3520.10111397728382579No Hit
CCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTTGGTCTTCCAAAC3520.10111397728382579No Hit
CCCCAAAAGACCGCCGTGAGGTGGGGTGAACCCTGGCCCGAATGCTCCTG3520.10111397728382579No Hit
CATTTGAACCCCAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT3510.10082672166654219No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)