FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490478

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490478
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences428081
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA20020.4676685019891096No Hit
GTGCTGGTACGCAGACCAATTTATGCCTACAGCCTCCCAATACAAAGACC18310.42772279078024955No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACTACACATAGCGCC14410.3366185371460074No Hit
CCTCCTGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA13590.31746328381778216No Hit
CTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGAA11050.2581287186303527No Hit
ATATAAGACCTTGGGCAATAATTGGTGGGCGTTCACGGTGGTCTCCATGC10360.2420102737566021No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATGGCAGAGAGTCCAA10300.24060866985453686No Hit
GGAATATGATAAAACGCCGCAGACACATCCAGCGATAACCAGGACAAGTT10300.24060866985453686No Hit
GTCTTAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCTGC9840.2298630399387032No Hit
CATCCTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATACTGCGCGGG9780.22846143603663793No Hit
CTCTAAGACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA8790.2053349716525611No Hit
ATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATTTACAC8590.20066295864567688No Hit
CTCTTCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAA8400.19622454628913685No Hit
CCCTTATCCAATGGCAAATATTTGGTAACTTTTGGATAAAACCTAGCAGG8320.1943557410863832No Hit
GTTCCGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA8200.19155253328225266No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT7270.16982767280024108No Hit
GTTCAGCGCAAGGTCCCCAATCCTCGAGAAGATTGACGATATGTGAGAGA7170.16749166629679899No Hit
CACTAATATGGGCCTAAAGTTTCGGCAAATATTGTGGTTTCACATTTCTT7080.16538926044370109No Hit
ATCATCTTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT7020.1639876565416358No Hit
CATCTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT7000.1635204552409474No Hit
CTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCT6920.1616516500381937No Hit
CTGTCTGATGTGATCTTGTGGCAATGACCCATGACATCCAATGACATAGC6900.1611844487375053No Hit
GTCACAATATTCTTGGGAACAAGAGCTACATCATGGGGCAGAATCTTTCC6870.16048364678647264No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC6720.1569796370313095No Hit
TCACCATACTGCACTCAGGCAAGCCATTCTTTGCTGGGGGGAACTAATGA6630.15487723117821162No Hit
CATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGGC6610.1544100298775232No Hit
GAAACGATGTATATTTCCGGGAGAGAACAACAGAGTTATCCGTCCCAAGA6490.15160682207339266No Hit
ATATAAGAGAGAAACTACACATAGCGCCTCATTTTGTGGGTCACAATATT6420.14997161752098317No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTACTGACTTCTTTCCTTCAGTACG6330.14786921166788528No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT6200.14483240321341054No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG6150.1436643999616895No Hit
GGTGTTTTGAGCAGAAGTCGTGCAGGTTCTGCATGGTCCCGTGCTGGTGG6100.14249639670996844No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATGTTGGATCGTC6080.14202919540928002No Hit
GTGTAGATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA5900.13782438370308422No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC5840.13642277980101897No Hit
ACTGGATACTGCGCGGGACGTCCTTTGTTTACGTCCCGTCGGCGCTGAAT5840.13642277980101897No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5820.13595557850033055No Hit
GGTAGAGGCAGGAGGCGGATTTGCTGGCAAGGTTTGTAGTATGCCCTGAG5650.13198436744447897No Hit
GTAGAAGAGTAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT5530.12918115964034846No Hit
ATGCTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG5520.12894755899000424No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5440.12707875378725053No Hit
CAAGCATTGGTGGGCGTTCACGGTGGTCTCCATGCGACGTGCAGAGGTGA5400.1261443511858737No Hit
GTATCGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC5260.12287394208105476No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5260.12287394208105476No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG5200.12147233817898949No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG5180.12100513687830106No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT5170.12077153622795686No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5160.12053793557761265No Hit
CTATCTCCACCTTTGAGAAACACTCATCCTCAGGCCATGCAGTGGAATTC5150.12030433492726843No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT5070.11843552972451475No Hit
ATGTAAGACCTTGGGCAAGCATTGGTGGGCGTTCACGGTGGTCTCCATGC5020.1172675264727937No Hit
GCTCTATATCGGGAAGCCTTAGAGTCTCCTGAGCATTGCTCACATCACCA5020.1172675264727937No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5020.1172675264727937No Hit
TTCTCGGGGGAACTACCGTGTGTCGTGGCCAAAATTCGCAGTCCCCAACC5010.11703392582244948No Hit
CCATTGAACAAAGGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGG4990.11656672452176108No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTAACCAATTTTCTTTTGTC4990.11656672452176108No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACTTTTGGATAAAAC4960.11586592257072843No Hit
ATCCAACATCCAGGGACCTAGTAGTCAGTTATGTTAACACTAATATGGGC4920.1149315199693516No Hit
CTACTGTTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCG4890.11423071801831897No Hit
ATATACATCTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGC4800.11212831216522107No Hit
TCATCTTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTT4790.11189471151487684No Hit
CTTCTAGATACCGCCTCAGCTCTATATCGGGAAGCCTTAGAGTCTCCTGA4770.11142751021418844No Hit
GCGCCGAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTGCTCGT4740.1107267082631558No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC4700.10979230566177897No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC4630.10815710110936949No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4610.10768989980868106No Hit
AGCAGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTAAGA4610.10768989980868106No Hit
CTCTATCTCCACCTTTGAGAAACACTCATCCTCAGGCCATGCAGTGGAAT4590.10722269850799264No Hit
CTTTAGGACATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGA4560.10652189655696001No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT4530.10582109460592738No Hit
CCTTTAATCTAAGCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAG4520.10558749395558316No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACATCACCATACTGCACTCAGGCA4490.10488669200455053No Hit
GTCATAGGCCATCAGCGCATGCGTGGAACCTTTCTGGCTCCTCTGCCGAT4470.10441949070386211No Hit
CTTATATAAGACCTTGGGCAATAATTGGTGGGCGTTCACGGTGGTCTCCA4430.10348508810248529No Hit
ATGAAAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCCAAAA4430.10348508810248529No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTAC4430.10348508810248529No Hit
ATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACCTG4430.10348508810248529No Hit
CTACTATTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCG4340.10138268224938737No Hit
ATAATTGGTGGGCGTTCACGGTGGTCTCCATGCGACGTGCAGAGGTGAAG4340.10138268224938737No Hit
GTGTGAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT4330.10114908159904318No Hit
CAGTAAACCCTGTTCCGACCACTGTCTCTCACATATCGTCAATCTTCTCG4320.10091548094869895No Hit
CAACAGTAGTCTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTGGTCTA4300.10044827964801054No Hit
ACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAA4290.10021467899766633No Hit
GTGCTGGCATCTGGTTGGCTTCTGGTGAGGCCTCAGTGACCTGTTACTCA4290.10021467899766633No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)