FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490479

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490479
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences428081
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA18970.4431404337029674No Hit
GTGCTGGTACGCAGACCAATTTATGCCTACAGCCTCCCAATACAAAGACC16920.3952523003824043No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACTACACATAGCGCC14490.3384873423487611No Hit
CATCCTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATACTGCGCGGG13370.3123240695102095No Hit
CCTCCTGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA13040.30461524804885054No Hit
GTCTTAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCTGC10030.23430145229524318No Hit
CTCTAAGACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA9420.22005181262424633No Hit
GGAATATGATAAAACGCCGCAGACACATCCAGCGATAACCAGGACAAGTT8650.20206456254774213No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATGGCAGAGAGTCCAA8590.20066295864567688No Hit
CTCTTCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAA8370.1955237443381042No Hit
GTTCCGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA8310.19412214043603898No Hit
CCCTTATCCAATGGCAAATATTTGGTAACTTTTGGATAAAACCTAGCAGG7700.17987250076504213No Hit
ATCATCTTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT7640.17847089686297687No Hit
GTTCAGCGCAAGGTCCCCAATCCTCGAGAAGATTGACGATATGTGAGAGA7610.17777009491194423No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG7270.16982767280024108No Hit
ATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATTTACAC7260.16959407214989686No Hit
ATATAAGACCTTGGGCAATAATTGGTGGGCGTTCACGGTGGTCTCCATGC7190.16795886759748738No Hit
CACTAATATGGGCCTAAAGTTTCGGCAAATATTGTGGTTTCACATTTCTT7110.1660900623947337No Hit
ACTGGATACTGCGCGGGACGTCCTTTGTTTACGTCCCGTCGGCGCTGAAT7100.1658564617443895No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATGTTGGATCGTC7040.16445485784232422No Hit
CTGTCTGATGTGATCTTGTGGCAATGACCCATGACATCCAATGACATAGC7000.1635204552409474No Hit
GTCACAATATTCTTGGGAACAAGAGCTACATCATGGGGCAGAATCTTTCC6930.16188525068853793No Hit
CTGCTAGGCTGTGCTGCCAACTGGATACTGCGCGGGACGTCCTTTGTTTA6770.15814764028303055No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT6710.1567460363809653No Hit
CATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGGC6630.15487723117821162No Hit
CATCTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT6600.15417642922717897No Hit
CTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCT6540.15277482532511372No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC6540.15277482532511372No Hit
GTGTAGATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA6430.15020521817132737No Hit
CTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGAA6330.14786921166788528No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT6140.14343079931134528No Hit
GAAACGATGTATATTTCCGGGAGAGAACAACAGAGTTATCCGTCCCAAGA6020.14062759150721477No Hit
ATATAAGAGAGAAACTACACATAGCGCCTCATTTTGTGGGTCACAATATT5990.13992678955618212No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC5920.13829158500377267No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT5750.13432037394792107No Hit
TCATCTTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTT5670.1324515687451674No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTACTGACTTCTTTCCTTCAGTACG5630.13151716614379055No Hit
TCACCATACTGCACTCAGGCAAGCCATTCTTTGCTGGGGGGAACTAATGA5610.13104996484310213No Hit
GTATCGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC5520.12894755899000424No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACTTTTGGATAAAAC5500.1284803576893158No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG5440.12707875378725053No Hit
GTAGAAGAGTAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT5410.12637795183621792No Hit
GCTCTATATCGGGAAGCCTTAGAGTCTCCTGAGCATTGCTCACATCACCA5390.1259107505355295No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT5390.1259107505355295No Hit
CTTTAGGACATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGA5290.12357474403208739No Hit
TTCTCGGGGGAACTACCGTGTGTCGTGGCCAAAATTCGCAGTCCCCAACC5220.12193953947967791No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTAACCAATTTTCTTTTGTC5200.12147233817898949No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5070.11843552972451475No Hit
GTCATAGGCCATCAGCGCATGCGTGGAACCTTTCTGGCTCCTCTGCCGAT5020.1172675264727937No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5020.1172675264727937No Hit
CATCATTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATACTGCGCGGG4960.11586592257072843No Hit
ATGCTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG4880.11399711736797474No Hit
ATCCAACATCCAGGGACCTAGTAGTCAGTTATGTTAACACTAATATGGGC4830.1128291141162537No Hit
GTCTTGGTGTAGATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGT4830.1128291141162537No Hit
GGATAACCACATTGTGTGAAAGGGGCAGCAAAACCCAAAAGACCCACAAT4820.1125955134659095No Hit
GGTAGAGGCAGGAGGCGGATTTGCTGGCAAGGTTTGTAGTATGCCCTGAG4780.11166111086453265No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGACATG4740.1107267082631558No Hit
CTATCTCCACCTTTGAGAAACACTCATCCTCAGGCCATGCAGTGGAATTC4730.11049310761281159No Hit
GCGCCGAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTGCTCGT4650.10862430241005791No Hit
CAGTAAACCCTGTTCCGACCACTGTCTCTCACATATCGTCAATCTTCTCG4640.10839070175971369No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC4640.10839070175971369No Hit
CCTTTAATCTAAGCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAG4630.10815710110936949No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC4600.10745629915833686No Hit
GGTGTTTTGAGCAGAAGTCGTGCAGGTTCTGCATGGTCCCGTGCTGGTGG4580.10698909785764844No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT4570.10675549720730423No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4570.10675549720730423No Hit
GTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG4540.10605469525627159No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACATCACCATACTGCACTCAGGCA4520.10558749395558316No Hit
ACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAA4510.10535389330523896No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4480.10465309135420633No Hit
ATCTCCACCTTTGAGAAACACTCATCCTCAGGCCATGCAGTGGAATTCCA4400.10278428615145266No Hit
ATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACCTG4390.10255068550110844No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA4380.10231708485076423No Hit
GTGCTGGGGCTGGGGTTGGAATTGAGATTGGGATCATCCTTACTAAAGGT4380.10231708485076423No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCT4350.1016162828997316No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTCGGGGGAACTACCGTG4340.10138268224938737No Hit
CCATTGAACAAAGGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGG4330.10114908159904318No Hit
ATATACATCTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGC4330.10114908159904318No Hit
CTCTATCTCCACCTTTGAGAAACACTCATCCTCAGGCCATGCAGTGGAAT4310.10068188029835476No Hit
AGCAGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTAAGA4300.10044827964801054No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)