FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490480

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490480
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences523347
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC27090.5176297943811659No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA24770.47329974185387513No Hit
ACTCACAACTGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT20090.38387532554882325No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC19650.3754679017936474No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAAAACCCGTG18930.3617102992851779No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGGCCCCCATG16920.3233036589490338No Hit
GTATACATGCATACAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAA15210.29062935299141873No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCATCAAACTCTTCAAG14500.27706282829556683No Hit
CATGTATACAAGCCAAACAGGCTTTTACTTTCTCGCCAATTTACAAGGCC14370.2745788167315376No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13260.25336917953098037No Hit
ATGTAAGACCGTTGTCAGGTTCCGGTGGGCGTTCACGGTGGTCTCCATGC12760.24381528890009876No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG11890.22719151920236477No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA11290.21572685044530682No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC10660.203688948250396No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC10630.20311571481254312No Hit
GTCCGGTGCTGGTGGTTGATGATCCTGGAATTAGAGGACAAACGCGCAAC10610.20273355918730787No Hit
GTGTAAAAGGGGCAGCGAACCCCAAAAGACCCACAATTCGTTGACATACT10550.20158709231160205No Hit
ATGTGGATATCCTGCTTTAATGCCTTTGTATGCATGTATACAAGCCAAAC10220.19528152449522018No Hit
GGATAATAGGGTTTGATTCCTTTATCTAAGGGCAAGTATTTGGTAACATT10210.19509044668260256No Hit
CTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT10140.19375290199427914No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG10050.19203320168072044No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT10040.19184212386810282No Hit
GCACCGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG9530.18209715542460356No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA9320.17808452135963326No Hit
ATATGTTCCTGTGGCAAGGAGCCCCAACCCCCAATTACATATCCCATGAA9110.174071887294663No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG9090.17368973166942775No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT8930.17063248666754563No Hit
TCCAAAGAGTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA8560.16356260760069324No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTT8520.1627982963502227No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAATTTCAT8490.1622250629123698No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG8460.1616518294745169No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG8290.15840350666001718No Hit
ATCTTATACAAAAGAGAGACAACACGTAGCGCCTCATTTTGCGGGTCACC8090.1545819504076645No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG8020.1532444057193411No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC8000.15286225009410584No Hit
GCCTAGCAGCCATGGAAACGATGTATACTTGCGGGAGAGCACTACAGAAT7850.14999608290484134No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC7780.14865853821651792No Hit
TATCCACATTGTGTAAAAGGGGCAGCGAACCCCAAAAGACCCACAATTCG7780.14865853821651792No Hit
GTACAGGGTCCCCAGTCTTCGAGAAGGTTGACGATATGGCAGAGGCAGTA7730.14770314915342975No Hit
ATCTAAGGGCAAGTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAA7640.14598344883987108No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA7620.1456012932146358No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG7610.14541021540201818No Hit
GTGGATATCCTGCTTTAATGCCTTTGTATGCATGTATACAAGCCAAACAG7590.1450280597767829No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7570.14464590415154763No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA7560.14445482633893No Hit
ATCAAGGTATGTTGCGCGTTTGTCCTCTAATTCCAGGATCATCAACCACC7480.14292620383798896No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC7460.14254404821275368No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA7260.13872249196040104No Hit
GCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATAAGGGAGTAGGCTGCCT7200.13757602508469524No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG7080.13528309133328367No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTCC7070.13509201352066602No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCGGTG7070.13509201352066602No Hit
CTACAGAATTGTCAGTCCCGATAAGTTTCGCTCCAGACCGGCTGCGAGCA7060.1349009357080484No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC7050.13470985789543075No Hit
GCTTACACCTATCAACAATGTCTTCCTGCAAATGAATGTCAGGAAAAGAA7030.13432770227019547No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCGCGCATGCGCTGATGGCC6930.13241692414401918No Hit
GGTTTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTG6890.13165261289354863No Hit
CTCTTTGGAAGGCGGGTATCTTATACAAAAGAGAGACAACACGTAGCGCC6890.13165261289354863No Hit
GTATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGTCTGGA6870.13127045726831338No Hit
ACCAAATACTTGCCCTTAGATAAAGGAATCAAACCCTATTATCCAGAGCA6840.13069722383046048No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT6820.13031506820522523No Hit
GGAACATATTGTACAAAAAATCAAATCATGTTTTAGGAAACTTCCTGTAA6790.12974183476737233No Hit
CTTTTACACAATGTGGATATCCTGCTTTAATGCCTTTGTATGCATGTATA6790.12974183476737233No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG6750.12897752351690178No Hit
CCTATCAACAATGTCTTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT6720.12840429007904888No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA6670.12744890101596074No Hit
GTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG6640.12687566757810784No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG6620.12649351195287256No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC6620.12649351195287256No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACCT6590.12592027851501966No Hit
CCCTTAGATAAAGGAATCAAACCCTATTATCCAGAGCATGTAGTTAATCA6550.12515596726454914No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG6490.12400950038884336No Hit
CAGTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT6450.12324518913837282No Hit
ATGTATACTTGCGGGAGAGCACTACAGAATTGTCAGTCCCGATAAGTTTC6400.12228980007528466No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC6390.12209872226266703No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGTGGATTGGGGACAGAAAGAT6360.12152548882481413No Hit
GTTGATAGGTGTAAGCAATTTGTGGGACCCCTAACAATAAATGAAAACAG6210.11865932163554964No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG6210.11865932163554964No Hit
ATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATTTACAT6210.11865932163554964No Hit
TCCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCACT6200.118468243822932No Hit
CTTCTGGACTATCAAGGTATGTTGCGCGTTTGTCCTCTAATTCCAGGATC6150.11751285475984385No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACATGATTTGATTTTTTGT6130.11713069913460859No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAATTGGCGA6110.11674854350937332No Hit
ATACAAAAGAGAGACAACACGTAGCGCCTCATTTTGCGGGTCACCATATT6100.1165574656967557No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA6070.11598423225890278No Hit
GGTCTATGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCTTGGC6060.11579315444628516No Hit
GTTTACAGGAAGTTTCCTAAAACATGATTTGATTTTTTGTACAATATGTT6050.11560207663366753No Hit
ATTTTGTGGTCTATATGCGGGAGGAGTGCGAATCCACACTCCAAAAGACA6040.11541099882104991No Hit
GCTCAGAATACTGCCTCTGCCATATCGTCAACCTTCTCGAAGACTGGGGA6030.11521992100843226No Hit
ATGTAATTGGGGGTTGGGGCTCCTTGCCACAGGAACATATTGTACAAAAA6030.11521992100843226No Hit
GTCCTAGGGGTCCTGATGCGATGTTCTCCATGTTCGGTACAGGGTCCCCA6010.114837765383197No Hit
GTTCCTGAACTGGAGCCACCAGCAGGAAAGTACAGAGCCCTGACTCTGGG5990.11445560975796172No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGT5980.1142645319453441No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG5950.1136912985074912No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA5910.11292698725702069No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGA5880.11235375381916779No Hit
GTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGG5850.11178052038131488No Hit
GTCTATATGCGGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATAT5850.11178052038131488No Hit
ATTTACATACTCTTTGGAAGGCGGGTATCTTATACAAAAGAGAGACAACA5800.11082513131822672No Hit
GGTGTAAGCAATTTGTGGGACCCCTAACAATAAATGAAAACAGGAGATTA5740.10967866444252093No Hit
CTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACA5710.10910543100466803No Hit
GCACTACAGAATTGTCAGTCCCGATAAGTTTCGCTCCAGACCGGCTGCGA5680.10853219756681513No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCATATAGACCACCAAATGCC5670.1083411197541975No Hit
CCTCCCGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5640.1077678863163446No Hit
GTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTATGCCAAG5630.10757680850372697No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT5610.10719465287849171No Hit
GGATATCCACATTGTGTAAAAGGGGCAGCGAACCCCAAAAGACCCACAAT5560.10623926381540354No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTATGCCAAGTGTTTGCTG5520.10547495256493303No Hit
ATATGCGGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA5420.10356417443875669No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT5360.10241770756305091No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAATCTCCTCC5350.10222662975043328No Hit
GTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGTAAGACCGTTGT5350.10222662975043328No Hit
CTCTTATGTAAGACCGTTGTCAGGTTCCGGTGGGCGTTCACGGTGGTCTC5320.10165339631258037No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT5310.10146231849996275No Hit
GTATGCATGTATACAAGCCAAACAGGCTTTTACTTTCTCGCCAATTTACA5310.10146231849996275No Hit
GGATTAAAGTTAGGTACCGTAGAAGAATAAAGCCCCGTAAAGTTTCCCAC5300.1012712406873451No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA5300.1012712406873451No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT5290.10108016287472747No Hit
CTTCAAAGACTGTGTGTTTACTGAGTGGGAGGAGTTGGGGGAGGAGATTA5260.10050692943687459No Hit
CAATTACATATCCCATGAAATTAAGGGAATATCCCCATCTTTTTGTTTTG5250.10031585162425694No Hit
GTCCAAGAGTCCTCTTATGTAAGACCGTTGTCAGGTTCCGGTGGGCGTTC5250.10031585162425694No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)