FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences523347
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC22840.43642172401867213No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAAAACCCGTG19180.3664872446006187No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA18760.3584619764706782No Hit
ACTCACAACTGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT18620.3557868870940313No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGGCCCCCATG17050.325787670513063No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC17020.32521443707521014No Hit
CATGTATACAAGCCAAACAGGCTTTTACTTTCTCGCCAATTTACAAGGCC14240.2720948051675084No Hit
GTATACATGCATACAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAA13960.26674462641421465No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCATCAAACTCTTCAAG13880.2652160039132736No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13230.2527959460931275No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG12620.24114019952345195No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA11030.21075882731724838No Hit
GTCCGGTGCTGGTGGTTGATGATCCTGGAATTAGAGGACAAACGCGCAAC10310.19700122480877888No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT10250.19585475793307305No Hit
CTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT10090.19279751293119096No Hit
ATGTGGATATCCTGCTTTAATGCCTTTGTATGCATGTATACAAGCCAAAC10020.19145996824286754No Hit
GGATAATAGGGTTTGATTCCTTTATCTAAGGGCAAGTATTTGGTAACATT10010.19126889043024992No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC9780.18687410074004437No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT9670.1847722448012504No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG9210.17598266542083935No Hit
GTGTAAAAGGGGCAGCGAACCCCAAAAGACCCACAATTCGTTGACATACT9170.1752183541703688No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAATTTCAT9160.17502727635775117No Hit
GCACCGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG8970.17139679791801615No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA8190.15649272853384083No Hit
ATCTAAGGGCAAGTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAA8040.15362656134457636No Hit
ATATGTTCCTGTGGCAAGGAGCCCCAACCCCCAATTACATATCCCATGAA8000.15286225009410584No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG7860.15018716071745897No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT7760.14827638259128265No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA7760.14827638259128265No Hit
GTGGATATCCTGCTTTAATGCCTTTGTATGCATGTATACAAGCCAAACAG7750.14808530477866502No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG7700.14712991571557685No Hit
ATGTAAGACCGTTGTCAGGTTCCGGTGGGCGTTCACGGTGGTCTCCATGC7670.14655668227772395No Hit
ATCTTATACAAAAGAGAGACAACACGTAGCGCCTCATTTTGCGGGTCACC7660.14636560446510632No Hit
GCTTACACCTATCAACAATGTCTTCCTGCAAATGAATGTCAGGAAAAGAA7460.14254404821275368No Hit
GCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATAAGGGAGTAGGCTGCCT7430.14197081477490078No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC7420.14177973696228313No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA7410.1415886591496655No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG7290.1392957253982539No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA7260.13872249196040104No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTCC7230.13814925852254814No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC7190.13738494727207762No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC7190.13738494727207762No Hit
GCCTAGCAGCCATGGAAACGATGTATACTTGCGGGAGAGCACTACAGAAT7170.13700279164684234No Hit
GTACAGGGTCCCCAGTCTTCGAGAAGGTTGACGATATGGCAGAGGCAGTA7120.1360474025837542No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG7120.1360474025837542No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC7100.13566524695851892No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG7090.1354741691459013No Hit
TCCAAAGAGTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA7080.13528309133328367No Hit
ATCAAGGTATGTTGCGCGTTTGTCCTCTAATTCCAGGATCATCAACCACC6900.13184369070616625No Hit
TATCCACATTGTGTAAAAGGGGCAGCGAACCCCAAAAGACCCACAATTCG6870.13127045726831338No Hit
GTATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGTCTGGA6840.13069722383046048No Hit
GGAACATATTGTACAAAAAATCAAATCATGTTTTAGGAAACTTCCTGTAA6820.13031506820522523No Hit
GTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG6780.12955075695475468No Hit
CTCTTTGGAAGGCGGGTATCTTATACAAAAGAGAGACAACACGTAGCGCC6760.1291686013295194No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA6700.12802213445381364No Hit
CTTTTACACAATGTGGATATCCTGCTTTAATGCCTTTGTATGCATGTATA6640.12687566757810784No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTT6620.12649351195287256No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC6610.12630243414025494No Hit
GTTGATAGGTGTAAGCAATTTGTGGGACCCCTAACAATAAATGAAAACAG6580.12572920070240204No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC6500.12420057820146098No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG6460.12343626695099044No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC6430.12286303351313757No Hit
CTTCTGGACTATCAAGGTATGTTGCGCGTTTGTCCTCTAATTCCAGGATC6420.12267195570051992No Hit
GGTTTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTG6390.12209872226266703No Hit
GCTCAGAATACTGCCTCTGCCATATCGTCAACCTTCTCGAAGACTGGGGA6370.12171656663743176No Hit
ATTCTGTAGTGCTCTCCCGCAAGTATACATCGTTTCCATGGCTGCTAGGC6360.12152548882481413No Hit
GGTCTATGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCTTGGC6360.12152548882481413No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCGGTG6280.11999686632387307No Hit
GTTTACAGGAAGTTTCCTAAAACATGATTTGATTTTTTGTACAATATGTT6280.11999686632387307No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGTGGATTGGGGACAGAAAGAT6270.11980578851125544No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG6260.11961471069863781No Hit
CCTATCAACAATGTCTTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT6250.11942363288602016No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6250.11942363288602016No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA6240.11923255507340254No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT6230.11904147726078491No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACATGATTTGATTTTTTGT6210.11865932163554964No Hit
ATGTAATTGGGGGTTGGGGCTCCTTGCCACAGGAACATATTGTACAAAAA6150.11751285475984385No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCGCGCATGCGCTGATGGCC6120.11693962132199094No Hit
CCCTTAGATAAAGGAATCAAACCCTATTATCCAGAGCATGTAGTTAATCA6050.11560207663366753No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA6040.11541099882104991No Hit
ACCAAATACTTGCCCTTAGATAAAGGAATCAAACCCTATTATCCAGAGCA6040.11541099882104991No Hit
ATTTTGTGGTCTATATGCGGGAGGAGTGCGAATCCACACTCCAAAAGACA6040.11541099882104991No Hit
GAATACTGCCTCTGCCATATCGTCAACCTTCTCGAAGACTGGGGACCCTG6030.11521992100843226No Hit
GTCTATATGCGGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATAT6030.11521992100843226No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG5960.11388237632010885No Hit
GTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGG5920.11311806506963831No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCATGCGCGGAACCTTTGTGTC5920.11311806506963831No Hit
CTTCAAAGACTGTGTGTTTACTGAGTGGGAGGAGTTGGGGGAGGAGATTA5920.11311806506963831No Hit
GTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTATGCCAAG5900.11273590944440304No Hit
CTACAGAATTGTCAGTCCCGATAAGTTTCGCTCCAGACCGGCTGCGAGCA5900.11273590944440304No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5890.11254483163178541No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGA5850.11178052038131488No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACCT5810.11101620913084435No Hit
GTCCTAGGGGTCCTGATGCGATGTTCTCCATGTTCGGTACAGGGTCCCCA5780.11044297569299147No Hit
GGTGTAAGCAATTTGTGGGACCCCTAACAATAAATGAAAACAGGAGATTA5680.10853219756681513No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAATTGGCGA5670.1083411197541975No Hit
CTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACA5650.10795896412896225No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTATGCCAAGTGTTTGCTG5640.1077678863163446No Hit
ATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATTTACAT5550.10604818600278591No Hit
ATGTATACTTGCGGGAGAGCACTACAGAATTGTCAGTCCCGATAAGTTTC5540.10585710819016828No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT5490.10490171912708013No Hit
AGATAAGGGAGTAGGCTGCCTTCCGGACTGCCGATTGGTGGAGGCAGGAG5480.10471064131446249No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG5430.10375525225137432No Hit
CCTCCCGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5420.10356417443875669No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCATATAGACCACCAAATGCC5400.10318201881352143No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA5350.10222662975043328No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT5340.10203555193781563No Hit
ATATGCGGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA5330.101844474125198No Hit
AGTATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG5270.10069800724949221No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG5270.10069800724949221No Hit
GGATTAAAGTTAGGTACCGTAGAAGAATAAAGCCCCGTAAAGTTTCCCAC5260.10050692943687459No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)