FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490482

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490482
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences472217
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGCACAGACCAATTTATGCCTACAGCCTCCTAATACAAAAATC33370.7066666384310603No Hit
GTACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG28870.6113714669315167No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAACCACCCGTG26740.566265085755066No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC24700.5230646080086062No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC23830.5046408748520278No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA16430.3479332594972227No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA14700.3112975602318426No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT14610.30939165680185166No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTCAG13750.29117969069305No Hit
CTATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATA13390.28355607697308655No Hit
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT13110.27762659963533715No Hit
ATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG12780.2706382870587039No Hit
CCTATTAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT12700.2689441506764898No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT12680.2685206165809363No Hit
ATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCCATGAA12350.2615323040043031No Hit
GTACAGCATCGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC11260.23844969579663589No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA11250.2382379287488591No Hit
TCCTCAGGCCATGCAATGGAATTCCACTGCCTTCCACCAAGCTCTGCAGG11080.23463788893665413No Hit
CATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC11080.23463788893665413No Hit
GTATAAAGGGACTCACGATGCTGTACAGTCTTGGCCCCCAATACCACATC9870.2090140761556657No Hit
CTGGTGCACAGACCAATTTATGCCTACAGCCTCCTAATACAAAAATCATT9750.20647287158234456No Hit
ATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATATT9470.20054339424459516No Hit
GCCTAATCATCTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGC9170.19419038281129228No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCATGTACTGTTTACTT8760.18550793385244496No Hit
GTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG8630.18275496223134702No Hit
GGATCATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA8560.18127259289690967No Hit
CCATTTACACAATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTAT8510.18021375765802586No Hit
TATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCT8460.17915492241914205No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACC8440.17873138832358854No Hit
GTACAGTCTTGGCCCCCAATACCACATCATCCATATAGCTGAAAGCCAAG8430.17851962127581175No Hit
AGACTGTACAGCATCGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT8300.17576664965471384No Hit
GCCTAGTCATCTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGC8230.17428428032027649No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGCCT8160.17280191098583914No Hit
ATACCACATCATCCATATAGCTGAAAGCCAAGCAGTGGGGGAAAGCCCTA8150.17259014393806235No Hit
ATGCTGTACAGTCTTGGCCCCCAATACCACATCATCCATATAGCTGAAAG8140.17237837689028562No Hit
CTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC8120.17195484279473208No Hit
ATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC8100.17153130869917854No Hit
GTACTGTAGAGGAATAAAGCCCAGTAAAATTTCCCACCTTATGAGTCCAA8080.17110777460362503No Hit
TTCCCCCACTGCTTGGCTTTCAGCTATATGGATGATGTGGTATTGGGGGC8040.17026070641251798No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTCCGCGAGAGGACGACAGAGT8030.17004893936474122No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA7890.16708420069586652No Hit
CTTTCGGAGTGTGGATTCGCACTCCTCCAGCCTATAGACCACCAAATGCC7890.16708420069586652No Hit
CTCCTAGACACCGTCTCAGCTCTGTTTCGAGATGCCTTAGAGTCTCCTGA7850.16623713250475947No Hit
ATGTTAGTATTAACATAATTGACTACTAGATCCCTGGATGCTGGATCTTG7810.1653900643136524No Hit
CTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTT7810.1653900643136524No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTGCACTCAGGCA7730.1636959279314383No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC7530.159460586975903No Hit
ATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAAC7520.15924881992812626No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG7460.1579782176414657No Hit
CTCTTTGGAAGGCTGGTATTCTATATAAGAGGGAAACCACACGTAGCGCA7420.1571311494503586No Hit
GTGGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAATACCGCCGTG7360.15586054716369804No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGAAAATACAGACCCCT7280.15416641078148394No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG7140.15120167211260924No Hit
CAGTAAACCCTGCTCCGAATATTGCCTCTCACATCTCGTCAATCTCCGCG7130.15098990506483248No Hit
GGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCA7120.15077813801705572No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT7120.15077813801705572No Hit
GGCTGGAGGAGTGCGAATCCACACTCCGAAAGAGAGCAAATATTCAAGTA7050.14929576868261837No Hit
GTAGAGGAATAAAGCCCAGTAAAATTTCCCACCTTATGAGTCCAAGGAAT6930.14675456410929721No Hit
TATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCT6840.14484866067930635No Hit
CTCTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT6830.14463689363152957No Hit
ATTGACCACAGTGTCAACAATTCCTCCTCCTGCCTCCACCAATCGGCAGT6810.14421335953597603No Hit
GTCTAAGGGCAAATATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAA6800.14400159248819927No Hit
GTCTTGGCCCCCAATACCACATCATCCATATAGCTGAAAGCCAAGCAGTG6730.14251922315376195No Hit
ATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTAT6720.14230745610598516No Hit
GGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTAC6610.13997801858044076No Hit
CTCCGAAAGAGAGCAAATATTCAAGTACAGTCTCTCTTCCAAAAATAATG6590.13955448448488725No Hit
ATGTGGTATTGGGGGCCAAGACTGTACAGCATCGTGAGTCCCTTTATACC6470.1370132799115661No Hit
CACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTATTATCCAGATC6440.1363779787682358No Hit
GTCCCCAGTCCTCGCGGAGATTGACGAGATGTGAGAGGCAATATTCGGAG6410.1357426776249055No Hit
ATTCTATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAAC6340.13426030829046814No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTGTGGGT6330.1340485412426914No Hit
GGGTAAAGGTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA6290.13320147305158433No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAA6230.13193087076492374No Hit
GTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAACAG6230.13193087076492374No Hit
CCTGTATTCCCATCCCATCGTCCTGGGCTTTCGCAAAATACCTATGGGAG6200.13129556962159347No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT6160.13044850143048642No Hit
GTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA6140.13002496733493288No Hit
GTCCTGGGCTTTCGCAAAATACCTATGGGAGTGGGCCTCAGTCCGTTTCT6120.12960143323937937No Hit
TCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTCCTCTACAG6070.12854259800049556No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTGTTTGATCTTTTGT6060.12833083095271877No Hit
TCACCATACTGCACTCAGGCAAGCCATTCTCTGCTGGGGGGAATTGATGA6060.12833083095271877No Hit
CCCATAGGTATTTTGCGAAAGCCCAGGACGATGGGATGGGAATACAGGTG6010.12727199571383496No Hit
ATACTGCACTCAGGCAAGCCATTCTCTGCTGGGGGGAATTGATGACTCTA6010.12727199571383496No Hit
CCTAGACACCGTCTCAGCTCTGTTTCGAGATGCCTTAGAGTCTCCTGAGC5980.12663669457050467No Hit
TTATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAG5910.12515432523606732No Hit
GCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTAC5890.12473079114051377No Hit
GTACAAAACCTACGGATGGAAATTGCACCTGTATTCCCATCCCATCGTCC5870.12430725704496028No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG5770.12218958656719263No Hit
GAATACAGGTGCAATTTCCATCCGTAGGTTTTGTACAGCAACATGAGGGA5680.12028368313720175No Hit
GCCTACTCCCATCTCTCCACCTCTAAGAGACAGTCATCCTCAGGCCATGC5640.1194366149460947No Hit
AGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTA5630.11922484789831794No Hit
GTCCAAGGAATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGAC5570.11795424561165735No Hit
GTATTAACATAATTGACTACTAGATCCCTGGATGCTGGATCTTGCAAATT5550.11753071151610382No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGCT5510.11668364332499677No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5480.11604834218166649No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGGTTCCCGAGATTGAGATCT5400.11435420579945237No Hit
TTATTATCCAGATCAGGTAGTTAATCATTACTTCCAAACCAGACATTATT5390.11414243875167561No Hit
GGATTAAAGATAGGTACTGTAGAGGAATAAAGCCCAGTAAAATTTCCCAC5350.11329537056056856No Hit
GTGTTTGATCTTTTGTACAATATGATCCTGTGGCAATGTTCCCCAACTTC5280.11181300122613122No Hit
CCCTTAGACAAAGGAATTAAACCTTATTATCCAGATCAGGTAGTTAATCA5270.11160123417835444No Hit
ACTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGATGGAAATTGC5260.11138946713057768No Hit
CAGTTGGACCCTGCATTCGGAGCCAACTCAAACAATCCAGATTGGGACTT5250.11117770008280091No Hit
GGATATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAAT5240.11096593303502415No Hit
GCCCAGGACGATGGGATGGGAATACAGGTGCAATTTCCATCCGTAGGTTT5240.11096593303502415No Hit
TGTATACCCAGAGACAAAAGAAAATTGGTAACAGCGGTATAAAGGGACTC5170.10948356370058682No Hit
TTCCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTC5070.10736589322281917No Hit
CCTCACCATACTGCACTCAGGCAAGCCATTCTCTGCTGGGGGGAATTGAT5050.10694235912726564No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACGACGGGAC4990.10567175684060505No Hit
GTGCACACGGACCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAA4980.1054599897928283No Hit
GTCCAACTGATGATCGGGAAAGAATCCCAGAGGGTTGGGAACAGAAAGAT4920.10418938750616771No Hit
GTACAATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCC4900.10376585341061419No Hit
CTGCTAGGCTGTACTGCCAACTGGATCCTTCGCGGGACGTCCTTTGTTTA4870.1031305522672839No Hit
GAATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4840.10249525112395362No Hit
ATGGTGAGGTGAGCAATGCTCAGGAGACTCTAAGGCATCTCGAAACAGAG4820.10207171702840008No Hit
GGAATTAGAGGACAAACGGGCAACATACCTTGATAATCCAGAAGAACCAA4800.10164818293284655No Hit
CTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAGATTCATTTACAAG4800.10164818293284655No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG4800.10164818293284655No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGG4780.10122464883729303No Hit
CTCCTACCTGGTTGGCTGCTGGCCAGTGGTCCTTGATGGGGTTGAAGTCC4760.1008011147417395No Hit
ATATGGATGATGTGGTATTGGGGGCCAAGACTGTACAGCATCGTGAGTCC4750.10058934769396274No Hit
ATGTATATTTCCGCGAGAGGACGACAGAGTTGTCAGTTCCGATAAGCTTT4740.10037758064618597No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)