FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences472217
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGCACAGACCAATTTATGCCTACAGCCTCCTAATACAAAAATC29700.6289481318969881No Hit
GTACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG27830.5893476939627332No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAACCACCCGTG26350.5580061708917722No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC21220.44936967538229244No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC20640.43708718661124013No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA16660.35280390159608827No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTCAG14020.29689740098302264No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT13370.283132542877533No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA13180.2791089689697745No Hit
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT12550.2657676449598384No Hit
ATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG12300.26047346876541927No Hit
CTATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATA12010.2543322243798931No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT11930.252638087997679No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA11890.251791019806572No Hit
CCTATTAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT11420.24183796856106407No Hit
ATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCCATGAA11260.23844969579663589No Hit
TCCTCAGGCCATGCAATGGAATTCCACTGCCTTCCACCAAGCTCTGCAGG10760.22786134340779768No Hit
GTACAGCATCGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC10460.22150833197449477No Hit
GCCTAATCATCTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGC10150.21494355349341512No Hit
GTATAAAGGGACTCACGATGCTGTACAGTCTTGGCCCCCAATACCACATC9850.20859054206011218No Hit
GTACAGTCTTGGCCCCCAATACCACATCATCCATATAGCTGAAAGCCAAG9280.19651982033683665No Hit
GCCTAGTCATCTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGC9130.1933433146201852No Hit
ATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATATT9060.19186094528574787No Hit
CATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC9040.19143741119019433No Hit
CTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC8960.18974327480798023No Hit
GGATCATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA8690.1840255645180076No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGCCT8600.18211966108801675No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACC8530.1806372917535794No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA8130.17216660984250887No Hit
CTGGTGCACAGACCAATTTATGCCTACAGCCTCCTAATACAAAAATCATT8110.17174307574695533No Hit
CTCCTAGACACCGTCTCAGCTCTGTTTCGAGATGCCTTAGAGTCTCCTGA7950.16835480298252709No Hit
CCATTTACACAATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTAT7920.16771950183919682No Hit
ATGCTGTACAGTCTTGGCCCCCAATACCACATCATCCATATAGCTGAAAG7900.16729596774364328No Hit
GTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG7870.166660666600313No Hit
CTTTCGGAGTGTGGATTCGCACTCCTCCAGCCTATAGACCACCAAATGCC7840.16602536545698268No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCATGTACTGTTTACTT7810.1653900643136524No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTGCACTCAGGCA7650.1620017915492242No Hit
TATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCT7590.1607311892625636No Hit
CTCTTTGGAAGGCTGGTATTCTATATAAGAGGGAAACCACACGTAGCGCA7560.16009588811923334No Hit
ATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAAC7490.15861351878479596No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT7410.15691938240258188No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG7400.1567076153548051No Hit
CTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTT7380.15628408125925156No Hit
GTGTTTGATCTTTTGTACAATATGATCCTGTGGCAATGTTCCCCAACTTC7260.15374287668593042No Hit
GTACTGTAGAGGAATAAAGCCCAGTAAAATTTCCCACCTTATGAGTCCAA7240.15331934259037688No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTCCGCGAGAGGACGACAGAGT7090.15014283687372543No Hit
AGACTGTACAGCATCGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT7080.14993106982594867No Hit
ATACCACATCATCCATATAGCTGAAAGCCAAGCAGTGGGGGAAAGCCCTA7040.14908400163484162No Hit
CAGTAAACCCTGCTCCGAATATTGCCTCTCACATCTCGTCAATCTCCGCG7010.14844870049151132No Hit
GGCTGGAGGAGTGCGAATCCACACTCCGAAAGAGAGCAAATATTCAAGTA7010.14844870049151132No Hit
CTCTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT7000.14823693344373456No Hit
GTCTAAGGGCAAATATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAA6960.14738986525262748No Hit
GTCTTGGCCCCCAATACCACATCATCCATATAGCTGAAAGCCAAGCAGTG6950.14717809820485073No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAA6940.14696633115707397No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC6930.14675456410929721No Hit
ATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC6880.1456957288704134No Hit
ATGTTAGTATTAACATAATTGACTACTAGATCCCTGGATGCTGGATCTTG6820.1444251265837528No Hit
TTCCCCCACTGCTTGGCTTTCAGCTATATGGATGATGTGGTATTGGGGGC6720.14230745610598516No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGAAAATACAGACCCCT6710.1420956890582084No Hit
TATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCT6660.1410368538193246No Hit
GGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTAC6640.14061331972377106No Hit
GTAGAGGAATAAAGCCCAGTAAAATTTCCCACCTTATGAGTCCAAGGAAT6590.13955448448488725No Hit
GTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAACAG6530.13828388219822668No Hit
ATTGACCACAGTGTCAACAATTCCTCCTCCTGCCTCCACCAATCGGCAGT6510.13786034810267314No Hit
ATGTGGTATTGGGGGCCAAGACTGTACAGCATCGTGAGTCCCTTTATACC6490.1374368140071196No Hit
CTCCGAAAGAGAGCAAATATTCAAGTACAGTCTCTCTTCCAAAAATAATG6470.1370132799115661No Hit
GGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCA6390.13531914352935195No Hit
GTGGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAATACCGCCGTG6390.13531914352935195No Hit
GGGTAAAGGTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA6340.13426030829046814No Hit
ATTCTATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAAC6050.12811906390494202No Hit
ATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTAT6030.12769552980938847No Hit
CACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTATTATCCAGATC5950.12600139342717437No Hit
GCCTACTCCCATCTCTCCACCTCTAAGAGACAGTCATCCTCAGGCCATGC5940.1257896263793976No Hit
CCTAGACACCGTCTCAGCTCTGTTTCGAGATGCCTTAGAGTCTCCTGAGC5940.1257896263793976No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTGTGGGT5890.12473079114051377No Hit
TTATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAG5780.12240135361496939No Hit
GTCCCCAGTCCTCGCGGAGATTGACGAGATGTGAGAGGCAATATTCGGAG5700.12070721723275528No Hit
GTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA5690.12049545018497851No Hit
GTCCTGGGCTTTCGCAAAATACCTATGGGAGTGGGCCTCAGTCCGTTTCT5680.12028368313720175No Hit
TCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTCCTCTACAG5640.1194366149460947No Hit
CCCATAGGTATTTTGCGAAAGCCCAGGACGATGGGATGGGAATACAGGTG5620.11901308085054117No Hit
CCTGTATTCCCATCCCATCGTCCTGGGCTTTCGCAAAATACCTATGGGAG5610.11880131380276442No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG5520.11689541037277354No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTGTTTGATCTTTTGT5520.11689541037277354No Hit
GCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTAC5500.11647187627722001No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGCT5390.11414243875167561No Hit
ATACTGCACTCAGGCAAGCCATTCTCTGCTGGGGGGAATTGATGACTCTA5360.11350713760834531No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5330.11287183646501503No Hit
CCTCACCATACTGCACTCAGGCAAGCCATTCTCTGCTGGGGGGAATTGAT5290.11202476827390796No Hit
ACTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGATGGAAATTGC5240.11096593303502415No Hit
TCACCATACTGCACTCAGGCAAGCCATTCTCTGCTGGGGGGAATTGATGA5210.11033063189169386No Hit
GTCCAAGGAATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGAC5160.10927179665281003No Hit
GTATTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGCTCCGA5110.10821296141392622No Hit
TTATTATCCAGATCAGGTAGTTAATCATTACTTCCAAACCAGACATTATT5100.10800119436614945No Hit
GGATTAAAGATAGGTACTGTAGAGGAATAAAGCCCAGTAAAATTTCCCAC5070.10736589322281917No Hit
AGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTA5010.10609529093615858No Hit
GCCCAGGACGATGGGATGGGAATACAGGTGCAATTTCCATCCGTAGGTTT4970.10524822274505155No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG4960.10503645569727477No Hit
GGATATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAAT4960.10503645569727477No Hit
GTACAAAACCTACGGATGGAAATTGCACCTGTATTCCCATCCCATCGTCC4940.10461292160172124No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGGTTCCCGAGATTGAGATCT4940.10461292160172124No Hit
GGAGTAGGCTGCCTTCCTGACTGCCGATTGGTGGAGGCAGGAGGAGGAAT4940.10461292160172124No Hit
GTCCAACTGATGATCGGGAAAGAATCCCAGAGGGTTGGGAACAGAAAGAT4930.10440115455394448No Hit
GCTTTGGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGG4910.10397762045839096No Hit
CTGCTAGGCTGTACTGCCAACTGGATCCTTCGCGGGACGTCCTTTGTTTA4900.10376585341061419No Hit
CTCCTACCTGGTTGGCTGCTGGCCAGTGGTCCTTGATGGGGTTGAAGTCC4860.10291878521950715No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGG4860.10291878521950715No Hit
GTACAATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCC4840.10249525112395362No Hit
GAATACAGGTGCAATTTCCATCCGTAGGTTTTGTACAGCAACATGAGGGA4790.10143641588506978No Hit
ATATGGATGATGTGGTATTGGGGGCCAAGACTGTACAGCATCGTGAGTCC4790.10143641588506978No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG4790.10143641588506978No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)