FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490484

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490484
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423595
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA31540.7445791380918094No Hit
AGTCTGAGGACAGGAGGTTGGTGAGTGATTGGAGGTTGGGGACTGCGAAT23970.5658707019676814No Hit
GTAGATGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT17810.42044877772400524No Hit
TCCCTAGATTGAGATCTTCTGCGACGCGGCGATTGAGATCTGCGTCTGCG16360.38621796763417887No Hit
CCTCAGACTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGTTTTATCAT16200.3824407747966808No Hit
CCTCCAATTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGTTTTATCAT16060.37913573106386994No Hit
GTTTAATCTTTTGTACAATATGATCCTGTGGCAATGTTCCCCAACTTCCA15980.37724713464512094No Hit
GTGGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAATACCGCCGTG15470.3652073324755958No Hit
ATTCCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTT15150.3576529468005996No Hit
TCCAAGGATCTAGTGGTCAATTATGTTAATACTAACATGGGTTTAAAGAT14350.3387669826131092No Hit
AATTGGAGGACAGGAGGTTGGTGAGTGATTGGAGGTTGGGGACTGCGAAT13710.3236582112631169No Hit
TATTAACCACAGTGTCAACAATTCCTCCTCCTGCCTCCACCAATCGGCAG12980.3064247689420319No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC12910.3047722470756265No Hit
AGTTTGCAGTACAGCCTAGCAGCCATGGAAACGATGTATATTTCCGCGAG12620.2979260850576612No Hit
GGCAACTATTGTGGTTTCATATATCTTGCCTTACTTTTGGAAGAGAGACT12280.2898995502779778No Hit
GTACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG11610.2740825552709546No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10980.25920985847330585No Hit
CTCCGTCAACAGTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGG10470.24717005630378072No Hit
GTTTACTGAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGTTTA9790.23111698674441386No Hit
GGTACTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTG9290.21931325912723237No Hit
GGTGACCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTG8900.21010635158583083No Hit
ATATTTGCCTGATCTTTAAACCCATGTTAGTATTAACATAATTGACTACT8780.20727345695770721No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC8550.20184374225380378No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA8530.20137159314911646No Hit
CTATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATA8330.19665010210224387No Hit
CAGTAAGAGGGCCCACGAATTGTTGACACCTATTAATAATGTCCTCTTGT8190.19334505836943308No Hit
ATCTCGGGAATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAAC8020.18933179097959135No Hit
ATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCCATGAA8010.18909571642724773No Hit
CCTATTAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT7940.18744319456084232No Hit
GGCTGGAGGAGTGCGAATCCACACTCCAAAAGAGACCAAATATTCAAGTA7910.18673497090381141No Hit
ACTACGGACTTACTCTCGTTTTTGCCTCATGACTTCTATCCTTCCGTCAG7760.18319385261865698No Hit
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT7720.18224955440928245No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCAGCCTATAGACCACCAAATGCC7640.18036095799053342No Hit
AGTCTGTACAGCATCGTGAGGCCCTTTATACCGCTGTTACCAATTTTCTT7530.17776413791475348No Hit
GGTGATCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTG7420.17516731783897355No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAGCTGAAAGCCAAA7350.17351479597256814No Hit
GTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGTTTA7340.17327872142022452No Hit
ATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATATT7210.17020975223975732No Hit
AGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGGCCTATTGATTGG7120.16808508126866464No Hit
CCCAAAGTCAGGGGTCTGTATTTTCCTGCTGGTGGCTCCAGTTCAGGAAC7080.16714078305929012No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT6930.16359966477413568No Hit
GTGTTTAATCTTTTGTACAATATGATCCTGTGGCAATGTTCCCCAACTTC6790.16029462104132486No Hit
ATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTACAGTGAGA6610.1560452790991395No Hit
GTATAAAGGGCCTCACGATGCTGTACAGACTTGGCCCCCAATACCACATC6490.15321238447101596No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAAATC6470.1527402353663287No Hit
GATCCAGCATCCAGGGATCTAGTAGTCAATTATGTTAATACTAACATGGG6350.14990734073820514No Hit
ATTAACCACAGTGTCAACAATTCCTCCTCCTGCCTCCACCAATCGGCAGT6240.1473105206624252No Hit
CAGTAAACCCTGCTCCGAATATTGCCTCTCACATCTCGTCAATCTCCGCG6200.14636622245305067No Hit
TCCTCAGGCCATGCAGTGGAATTCCACTGCCTTCCACCAAGCTCTGCAGG6180.14589407334836343No Hit
GTGGTTAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAATACCGCCGTG6160.14542192424367614No Hit
ATTCTTTGACATACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTCCT5970.14093650774914718No Hit
GAACAAATGGCACTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCAC5780.1364510912546182No Hit
ATTAATAGGTGTCAACAATTCGTGGGCCCTCTCACTGTAAATGAAAAGAG5740.13550679304524368No Hit
CCTCAAATTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGTTTTATCAT5700.13456249483586916No Hit
GGGATCACCCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATC5630.13290997296946377No Hit
GTTTGATCTTTTGTACAATATGATCCTGTGGCAATGTTCCCCAACTTCCA5600.13220174931243286No Hit
CTAGCTACCTGGGTGGGTAATAATTTGCAAGATCCAGCATCCAGGGATCT5600.13220174931243286No Hit
ATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC5570.13149352565540198No Hit
ACATAGAGTTGCCTTGAGCAGGAGTCGTGCAGGTTTTGCATGGTCCCGTA5470.1291327801319657No Hit
GCCTAATCATCTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGC5390.12724418371321664No Hit
GTACAAAACCTACGGATGGAAATTGCACCTGTATTCCCATCCCATCGTCC5380.12700810916087302No Hit
CCTAATCTCCTCCCCCAGTTCCTCCCAGTCCTTAAACACACAGTCTTTGA5370.12677203460852937No Hit
ATTGACCACAGTGTCAACAATTCCTCCTCCTGCCTCCACCAATCGGCAGT5340.1260638109514985No Hit
CTCCAAAAGAGACCAAATATTCAAGTACAGTCTCTCTTCCAAAAGTAAGG5320.12559166184681123No Hit
CCCTTTACACAATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTAT5250.12393913998040582No Hit
GGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAATACCGCCGTGTG5220.12323091632337492No Hit
GCTCATCGGAACTGACAATTCTGTCGTCCTCTCGCGGAAATATACATCGT5220.12323091632337492No Hit
AGATCAAACACTGTTTTAGGAAACTTCCTGTTAACAGGCCTATTGATTGG5210.1229948417710313No Hit
CCTAATCTCCTCCCCCAGCTCCTCCCAGTCCTTAAACACACAGTCTTTGA5210.1229948417710313No Hit
GGGGATCACCCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAAT5110.12063409624759498No Hit
GCCTAGTCATCTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGC5070.11968979803822045No Hit
GGCTTGAGGAGTGCGAATCCACACTCCAAAAGAGACCAAATATTCAAGTA4980.1175651270671278No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGAAAATACAGACCCCT4940.11662082885775327No Hit
GTAGAGGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT4790.11307971057259883No Hit
GCCTTAATGCCTTTGTATGCATGTATACAAGCTAAACAGGCTTTCACTTT4760.11237148691556793No Hit
TCCAGGGATCTAGTAGTCAATTATGTTAATACTAACATGGGTTTAAAGAT4680.11048289049681889No Hit
CAGTGAGAGGGCCCACGAATTGTTGACACCTATTAATAATGTCCTCTTGT4670.11024681594447526No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACC4610.1088303686304135No Hit
CCTCAAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4490.10599747400228993No Hit
ACTGTGCAGTTACTCTCGTTTTTGCCTATAGACTTCTTTCCTTCCGTCGG4470.10552532489760266No Hit
CCCATAGGTATTTTGCGAAAGCCCAGGACGATGGGATGGGAATACAGGTG4390.10363672847885361No Hit
CCGTCAACAGTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTC4350.10269243026947909No Hit
CTCTCACTGTAAATGAAAAGAGAAGATTGAAATTAATTATGCCTGCCAGA4260.10056775929838642No Hit
CTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC4240.10009561019369917No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)