FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490485

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490485
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423595
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA30100.7105844025543266No Hit
AGTCTGAGGACAGGAGGTTGGTGAGTGATTGGAGGTTGGGGACTGCGAAT19020.4490137985575845No Hit
GTTTAATCTTTTGTACAATATGATCCTGTGGCAATGTTCCCCAACTTCCA15450.36473518337090854No Hit
TCCCTAGATTGAGATCTTCTGCGACGCGGCGATTGAGATCTGCGTCTGCG15210.3590693941146614No Hit
GTAGATGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT15060.35552827582950697No Hit
CCTCCAATTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGTTTTATCAT14950.35293145575372703No Hit
ATTCCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTT14720.3475017410498235No Hit
CCTCAGACTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGTTTTATCAT14250.33640623708967293No Hit
GTGGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAATACCGCCGTG12250.2891913266209469No Hit
TCCAAGGATCTAGTGGTCAATTATGTTAATACTAACATGGGTTTAAAGAT11980.2828173137076689No Hit
TATTAACCACAGTGTCAACAATTCCTCCTCCTGCCTCCACCAATCGGCAG11450.2703053624334565No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC11360.26818069146236384No Hit
GGCAACTATTGTGGTTTCATATATCTTGCCTTACTTTTGGAAGAGAGACT10490.247642205408468No Hit
GTACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG10400.2455175344373753No Hit
AATTGGAGGACAGGAGGTTGGTGAGTGATTGGAGGTTGGGGACTGCGAAT10090.2381992233147228No Hit
AGTTTGCAGTACAGCCTAGCAGCCATGGAAACGATGTATATTTCCGCGAG9960.2351302541342556No Hit
CTCCGTCAACAGTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGG9770.23064483763972662No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9550.22545119748816675No Hit
GTGTTTAATCTTTTGTACAATATGATCCTGTGGCAATGTTCCCCAACTTC9100.2148278426327034No Hit
ATATTTGCCTGATCTTTAAACCCATGTTAGTATTAACATAATTGACTACT9010.21270317166161073No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAGCTGAAAGCCAAA8660.20444056232958369No Hit
GTTTACTGAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGTTTA8650.20420448777724007No Hit
ATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCCATGAA8220.19405328202646396No Hit
GGCTGGAGGAGTGCGAATCCACACTCCAAAAGAGACCAAATATTCAAGTA8210.19381720747412032No Hit
GGTGACCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTG8080.19074823829365312No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC8010.18909571642724773No Hit
CTATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATA7620.17988880888584616No Hit
GGTACTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTG7600.1794166597811589No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCAGCCTATAGACCACCAAATGCC7510.17729198881006622No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA7430.17540339239131716No Hit
ACTACGGACTTACTCTCGTTTTTGCCTCATGACTTCTATCCTTCCGTCAG7230.17068190134444458No Hit
CCTATTAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT7180.16950152858272644No Hit
AGTCTGTACAGCATCGTGAGGCCCTTTATACCGCTGTTACCAATTTTCTT7060.16666863395460285No Hit
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT6980.16478003753585382No Hit
ATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATATT6980.16478003753585382No Hit
ATCTCGGGAATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAAC6970.16454396298351018No Hit
CCCAAAGTCAGGGGTCTGTATTTTCCTGCTGGTGGCTCCAGTTCAGGAAC6600.15580920454679587No Hit
CTGCCAACTGGATCCTTCGCGGGACGTCCTTTGTTTACGTCCCGTCGGCG6460.15250416081398505No Hit
GTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGTTTA6300.14872696797648696No Hit
GCCTAATCATCTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGC6190.14613014790070705No Hit
ATTCTTTGACATACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTCCT6180.14589407334836343No Hit
GTATAAAGGGCCTCACGATGCTGTACAGACTTGGCCCCCAATACCACATC6050.14282510416789623No Hit
CAGTAAGAGGGCCCACGAATTGTTGACACCTATTAATAATGTCCTCTTGT5900.13928398588274177No Hit
ATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTACAGTGAGA5900.13928398588274177No Hit
CAGTAAACCCTGCTCCGAATATTGCCTCTCACATCTCGTCAATCTCCGCG5860.13833968767336724No Hit
CTCCAAAAGAGACCAAATATTCAAGTACAGTCTCTCTTCCAAAAGTAAGG5840.13786753856868No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT5810.1371593149116491No Hit
GGTGATCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTG5740.13550679304524368No Hit
GTGGTTAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAATACCGCCGTG5700.13456249483586916No Hit
AGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGGCCTATTGATTGG5670.13385427117883827No Hit
GATCCAGCATCCAGGGATCTAGTAGTCAATTATGTTAATACTAACATGGG5640.1331460475218074No Hit
GTTTGATCTTTTGTACAATATGATCCTGTGGCAATGTTCCCCAACTTCCA5590.13196567476008925No Hit
CCTCAAATTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGTTTTATCAT5500.12984100378899657No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAAATC5490.12960492923665293No Hit
GAACAAATGGCACTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCAC5410.12771633281790387No Hit
TCCTCAGGCCATGCAGTGGAATTCCACTGCCTTCCACCAAGCTCTGCAGG5320.12559166184681123No Hit
ATTAACCACAGTGTCAACAATTCCTCCTCCTGCCTCCACCAATCGGCAGT5270.12441128908509307No Hit
ACATAGAGTTGCCTTGAGCAGGAGTCGTGCAGGTTTTGCATGGTCCCGTA5250.12393913998040582No Hit
GCCTAGTCATCTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGC5110.12063409624759498No Hit
ATTAATAGGTGTCAACAATTCGTGGGCCCTCTCACTGTAAATGAAAAGAG5090.12016194714290773No Hit
ATTGACCACAGTGTCAACAATTCCTCCTCCTGCCTCCACCAATCGGCAGT4970.11732905251478416No Hit
CCCTTTACACAATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTAT4880.1152043815436915No Hit
GTGTTTGATCTTTTGTACAATATGATCCTGTGGCAATGTTCCCCAACTTC4830.11402400878197336No Hit
GGGATCACCCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATC4830.11402400878197336No Hit
AGATCAAACACTGTTTTAGGAAACTTCCTGTTAACAGGCCTATTGATTGG4730.11166326325853704No Hit
GTATTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGCTCCGA4700.11095503960150616No Hit
GTACAAAACCTACGGATGGAAATTGCACCTGTATTCCCATCCCATCGTCC4640.10953859228744436No Hit
GGCTTGAGGAGTGCGAATCCACACTCCAAAAGAGACCAAATATTCAAGTA4600.10859429407806985No Hit
GGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAATACCGCCGTGTG4540.10717784676400807No Hit
GGGGATCACCCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAAT4480.10576139944994631No Hit
ACTGTGCAGTTACTCTCGTTTTTGCCTATAGACTTCTTTCCTTCCGTCGG4450.10505317579291541No Hit
GTAGAGGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT4440.10481710124057178No Hit
ATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC4440.10481710124057178No Hit
CTAGCTACCTGGGTGGGTAATAATTTGCAAGATCCAGCATCCAGGGATCT4400.10387280303119725No Hit
GCCTTAATGCCTTTGTATGCATGTATACAAGCTAAACAGGCTTTCACTTT4390.10363672847885361No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCACCCGTG4340.10245635571713546No Hit
GTCCCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAGATTCA4280.1010399084030737No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGAAAATACAGACCCCT4250.1003316847460428No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)