FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences320985
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA31590.9841581382307585No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC14370.4476844712369737No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA12930.40282256180195336No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACTACACATAGCGCC12800.3987725283112918No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT12700.3956571179338598No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA10000.3115410377431967No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT9410.2931601165163481No Hit
CTATGAATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC9260.28848700095020013No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATAATTAATCT8800.2741561132140131No Hit
CCTAATAATTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACA8530.2657445051949468No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATACGTTGACAGACT8360.2604483075533125No Hit
GTTAACAGACCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTT8110.2526597816097325No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTC7880.245494337741639No Hit
ATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTCACGGTGGTCTCCATGC7530.23459040142062715No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC7360.2292942037789928No Hit
ATATAAGAGAGAAACTACACATAGCGCCTCATTTTGTGGGTCACCATATT7350.22898266274124962No Hit
GAGAAACACTCATCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACC7160.22306338302412887No Hit
GTGTCTCTCAAAGGTGGAGACAGAGGGGTAGGCTGCCTTCCTGACTGGCG7110.22150567783541286No Hit
CCTAATAGTCAGTTATGTTAACACTAATATGGGCCTAAAATTTAGACGAC7050.2196364316089537No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6880.21434023396731935No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG6780.2112248235898874No Hit
GTCTATTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATGATG6580.20499400283502345No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC6530.20343629764630744No Hit
GATTCATAGAGGTTCTTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCC6490.20219013349533468No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC6480.2018785924575915No Hit
GGATCCTGCGCGGGACGTCCTTTGTTTACGTCCCGTCGGCGCTGAATCCC6420.2000093462311323No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT6340.19751701792918674No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAG6080.18941695094786362No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT5950.18536691745720205No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5720.1782014735891085No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5690.17726685047587892No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG5640.17570914528716294No Hit
CATTTAAACCCTAACAAAACGAAAAGATGGGGTTACTCTTTACATTTCAT5640.17570914528716294No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT5600.17446298113619016No Hit
GACTACTAGGTCCCTGGATATTGGATCTTCCAAATTACCACCCACCCAGG5480.1707244886832718No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA5470.1704129476455286No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC5330.16605137311712384No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG5280.16449366792840786No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC5240.16324750377743508No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5230.16293596273969188No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG4870.1517204853809368No Hit
TTATATAAGAGAGAAACTACACATAGCGCCTCATTTTGTGGGTCACCATA4860.1514089443431936No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC4850.1510974033054504No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG4770.14860507500350484No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTT4740.14767045189027525No Hit
GTCTCCTGAACATTGTTCACCTCACCATACTGCACTCAGGCAAGCAATTC4580.1426857952863841No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC4510.14050500802218172No Hit
CAATAGGTCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT4500.14019346698443852No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC4490.13988192594669532No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4480.13957038490895213No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT4440.13832422075797934No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG4370.13614343349377697No Hit
GGTTCTGCATGGTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGAC4360.13583189245603378No Hit
CTTTGGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGA4330.1348972693428042No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA4310.13427418726731777No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4300.1339626462295746No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA4270.133028023116345No Hit
GGATTCATAGAGGTTCTTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCC4270.133028023116345No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT4240.1320934000031154No Hit
CCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAAGAGTCCTCTTATGT4180.13022415377665625No Hit
CTTCTTTTCTCATTGACTGTGAGTGGGCCTACGAATTGTTCACATTTTTT4170.12991261273891302No Hit
GCTTAGATTGAATACATGCATACAAGGGCATTAAAGCAGGATAACCACAT4150.12928953066342663No Hit
GCTTTAATGCCCTTGTATGCATGTATTCAATCTAAGCAGGCTTTCACTTT4140.12897798962568344No Hit
GCAGTATCCAGTTGGCAGCACAGCCTAGCAGCCATGGAAACGATGTATAT4130.12866644858794024No Hit
TTAGAAAACTTCCTGTTAACAGACCTATTGATTGGAAAGTCTGTCAACGT4040.12586257924825148No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT4020.12523949717276509No Hit
ATCTTAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG4010.12492795613502188No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATGATTAATCT4010.12492795613502188No Hit
CCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGAAGTATGCCTCA4010.12492795613502188No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG3940.1227471688708195No Hit
AAACTACACATAGCGCCTCATTTTGTGGGTCACCATATTCTTGGGAACAA3890.12118946368210352No Hit
TTTCTGGCTCCTCTACCGATCCATACTGCGGAACTCCTAGCCGCTTGTTT3870.12056638160661713No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAAGAACCTCTATG3830.11932021745564435No Hit
GGCCTTTCTGTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAAC3780.11776251226692837No Hit
GACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTTTCGTTTTGTTAG3780.11776251226692837No Hit
CCATATAACTGAAAGCCAAACAATGGGGGAAAGCCCTACGAACCACTGAA3770.11745097122918516No Hit
GGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAG3760.11713943019144198No Hit
GCCAGGTCTGTGCCAAGTGTTTGCTGACGCAACCCCCACTGGCTGGGGCT3750.11682788915369877No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG3730.11620480707821237No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT3710.115581725002726No Hit
AATTCAGACAACTATTGTGGTTTCACATTTCTTGTCTCACTTTTGGAAGA3710.115581725002726No Hit
GCTCTATATCGGGAAGCCTTAGAGTCTCCTGAACATTGTTCACCTCACCA3700.11527018396498279No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA3690.11495864292723958No Hit
GTGCCAAGTGTTTGCTGACGCAACCCCCACTGGCTGGGGCTTGGTCATGG3630.11308939670078041No Hit
GGTCCTAGGAGTCCTGATGTGATGTTCTCCATGTTCAGCGCAGGGTCCCC3620.1127778556630372No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT3610.11246631462529402No Hit
GTATCCAGTTGGCAGCACAGCCTAGCAGCCATGGAAACGATGTATATTTG3600.11215477358755081No Hit
GTTTCCTAAAACATTCTTTGATTTTCTGTATGATGTGATCTTGTGGTAAG3590.11184323254980763No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA3580.11153169151206443No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG3570.11122015047432122No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT3570.11122015047432122No Hit
GCCTTTCTGTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACG3540.11028552736109165No Hit
ATCCCGCGGACGACCCTTCTCGGGGCCGCTTGGGACTCTCTCGTCCCCTT3540.11028552736109165No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG3530.10997398632334844No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA3530.10997398632334844No Hit
CATACTGCGGAACTCCTAGCCGCTTGTTTTGCTCGCAGCAGGTCTGGAGC3530.10997398632334844No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG3520.10966244528560525No Hit
GTATGGATCGGTAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC3480.10841628113463246No Hit
GGCCAGGTCTGTGCCAAGTGTTTGCTGACGCAACCCCCACTGGCTGGGGC3470.10810474009688925No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC3460.10779319905914607No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAATGGGGGAAAGCCCTA3450.10748165802140286No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA3430.10685857594591648No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTCGTAGGCCCACT3420.10654703490817327No Hit
GAGTTATCCGTCCCGAGAATGTTTGCTCCAGACCTGCTGCGAGCAAAACA3410.10623549387043009No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC3360.1046777886817141No Hit
GATCCTGCGCGGGACGTCCTTTGTTTACGTCCCGTCGGCGCTGAATCCCG3360.1046777886817141No Hit
CTATTGTGGTTTCACATTTCTTGTCTCACTTTTGGAAGAGAAACGGTCAT3340.10405470660622772No Hit
GAGTAACCCCATCTTTTCGTTTTGTTAGGGTTTAAATGTATACCCAAAGA3340.10405470660622772No Hit
ATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATTCAATCTAAGC3330.10374316556848451No Hit
CAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATTCAATCTAA3320.1034316245307413No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA3300.10280854245525492No Hit
TAATTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAG3300.10280854245525492No Hit
CATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC3290.10249700141751172No Hit
GTATGATGTGATCTTGTGGTAAGGACCCATAACATCCAATGACATAGCCC3240.10093929622879574No Hit
GTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTTGC3240.10093929622879574No Hit
CAGTAAACCCTGTTCCGACCACTGTCTCTCACATATCGTCAATCTTCTCG3220.10031621415330934No Hit
GACCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGCTTT3220.10031621415330934No Hit
GATCCATACTGCGGAACTCCTAGCCGCTTGTTTTGCTCGCAGCAGGTCTG3220.10031621415330934No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG3220.10031621415330934No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)