FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490489

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490489
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences464756
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTTGTTTGGAAGTAATGATTAACTAGA25660.5521176703474512No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC21600.4647600030984No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19410.41763850278425674No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC17950.38622416924149444No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC14670.31564950210433No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG14640.31500400210002666No Hit
CCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAACTCTGCAAG9950.21409083476060553No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG9520.20483866803225778No Hit
ACATAGAGGTGCCTTGAGCAGGCGTCGTGCAGGTTCTGCATGGTCCCGTG8570.1843978345626522No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT8430.18138550120923666No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC8000.17213333448088888No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGTCAGTC7840.16869066779127112No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC7790.16761483445076555No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAGCAATTTGTAGGCCCACT7730.1663238344421589No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7570.1628811677525411No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA7320.15750200105001333No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT7290.15685650104571No Hit
GCCTGAGGGTGAGTGTTTCTCAAAGGTGGAGACAGAGGGGTAGGCTGCCT7240.15578066770520446No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC7050.15169250101128334No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT7040.15147733434318222No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG6950.14954083433027224No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT6830.1469588343130589No Hit
GTCATAGAGTATTTAGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC6710.14437683429584558No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT6650.1430858342872389No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATA6320.13598533423990225No Hit
GCTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTTCTGTGG6050.1301758342011722No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5950.1280241675201611No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTTTTCAAGCCTCCAAGCTGTGC5950.1280241675201611No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA5850.12587250083915No Hit
AGTCTGTACAGCACCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT5740.12350566749003777No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA5580.12006300080042No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTTGT5560.11963266746421777No Hit
ATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTCACGGTGGTCTCCATGC5230.11253216741688112No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC5210.11210183408067889No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT5200.11188666741257779No Hit
GCACGGGACCATGCAGAACCTGCACGACGCCTGCTCAAGGCACCTCTATG5200.11188666741257779No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5170.11124116740827444No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5150.11081083407207222No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT5090.10951983406346556No Hit
CTTCTAGATACCGCCTCAGCTCTCTATCGGGAAGCCTTAGAGTCTCCTGA5080.10930466739536444No Hit
ATCCCAGAGTGAGAGGCCTGTATTTCCCTGCTGGTGGCTCCAGTTCAGGA5060.10887433405916222No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTTGTTT5030.10822883405485889No Hit
TCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAATTAATGA5010.10779850071865667No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC4970.10693783404625223No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAATTAATGACTTTA4960.10672266737815111No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC4930.10607716737384779No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTC4890.10521650070144334No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA4860.10457100069714001No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC4850.10435583402903889No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4840.10414066736093779No Hit
CAATAGGCCTGTTGACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT4690.10091316733942111No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACA4670.10048283400321889No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)