FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490490

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490490
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences360646
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT19460.5395872961297228No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA18100.5018771870476867No Hit
GGAATATGATAAAACGCCGCAGACACATCCAGCGATAACCAGGACAAGTT17280.4791402095129296No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC17110.4744264458776751No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAACAAC16670.46222611646878103No Hit
GTATCTCACTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA16000.4436483421416015No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG15810.43838001807866994No Hit
CCTCCAGCTTATAGACCATCAAATGCCCCTATCTTATCAACACTTCCGGA14340.3976198266444103No Hit
CTCTATGGACGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC13810.3829239753109698No Hit
GTCCCGTGCTGGTCGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC13540.37543740953733024No Hit
GGGAAATACAGGCCTTTCACTCTGGGATCTTGCAGAGTTTGGTGGAAAGT10060.2789438951215319No Hit
GAGACAAACTCATCCTCAGGCCGTGCAGTGGAACTCCACAACTTTCCACC9950.2758938127693084No Hit
ATATAGGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT9710.2692390876371844No Hit
GCCGAAAAGGTTCCACGCATGCGCTGATGGCCCATGACCAAGCCCCAGCC9240.25620691758677483No Hit
ATCATCTTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT8630.2392928245426263No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG8570.2376291432595953No Hit
ATGGACGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCCTCAT8080.22404241278150874No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA7860.21794224807706172No Hit
GCAGTGAGATACAGGCCTTTCACTCTGGGATCTTGCAGAGTTTGGTGGAA7730.21433760529716123No Hit
ATGTCATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAAT7700.2135057646556457No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC7360.20407823738513667No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG7300.20241455610210565No Hit
ACTTTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGACATGGACAT7190.19936447374988217No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG7090.19659167161149715No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA7020.19465071011462765No Hit
CCCAACAGACCTACAATACGTTGACAGACTTTCCAATCAATAGGCCTGTT6960.19298702883159663No Hit
GCCTGAAAGGTTCCACGCATGCGCTGATGGCCCATGACCAAGCCCCAGCC6950.19270974861775814No Hit
ATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGACT6850.18993694647937312No Hit
GTTTCTCTCAAAGGTGGAGACAGCGGAGTAGGCTGCCTTCCTGACTGGCG6750.1871641443409881No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT6740.1868868641271496No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6720.18633230369947262No Hit
GTGAGATACAGGCCTTTCACTCTGGGATCTTGCAGAGTTTGGTGGAAAGT6670.1849459026302801No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC6630.18383678177492613No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT6480.1796775785673486No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT6390.1771820566428021No Hit
CTACAAACCCTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC6360.17635021600128656No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT6280.17413197429057858No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC6260.17357741386290157No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA6190.17163645236603206No Hit
TCATCTTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTT6170.17108189193835507No Hit
GTCTTTTGGGTTTTGCTGCCCCTTTTACACAATGTGGTTATCCTGCTTTA6130.16997277108300105No Hit
CCCATAACATCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCATC6010.16664540851693907No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5880.16304076573703852No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATA5800.16082252402633054No Hit
GGGACTCACCCCACCGCACGGAGGCCTTTTGGGGTGGAGCCCTCAGGCTC5750.15943612295713802No Hit
CTCTTATATAATATACCCGCCGTCCATAGAGTGTGTAAATAATGTCTGGT5730.15888156252946103No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT5670.15721788124643002No Hit
CTTTAGGACATGGACATCGACCCTTATAAAGAATTTGGAGCTTCTGTGGA5670.15721788124643002No Hit
CTATGTATCCCTCCTGTTGCTGTACAAAACCTTCGGACGGAAATTGCACC5670.15721788124643002No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA5650.156663320818753No Hit
GTGTAAAAGGGGCAGCAAAACCCAACAGACCTACAATACGTTGACAGACT5600.1552769197495605No Hit
GTATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTACACCTCACCATACTG5370.148899474831275No Hit
TCACCATACTGCACTCAGGCAAGCAATTTTGTGCTGGGGGGAACTAATGA5330.14779035397592097No Hit
GAGTAGGCTGCCTTCCTGACTGGCGATTGGTAGAGGCAGGAGGCGGATTT5300.1469585133344055No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5250.145572112265213No Hit
GCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAAC4960.13753098606389647No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT4900.13586730478086545No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4900.13586730478086545No Hit
TTGTACACCTCACCATACTGCACTCAGGCAAGCAATTTTGTGCTGGGGGG4900.13586730478086545No Hit
CTGTTGCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACAATAGTTGC4890.13559002456702696No Hit
CTCTATGACTGTTGCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA4850.13448090371167293No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCC4710.1305989807179339No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG4690.13004442029025692No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4670.1294898598625799No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT4630.12838073900722594No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4600.1275488983657104No Hit
ATGCCTGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAG4600.1275488983657104No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC4580.1269943379380334No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACAAAACCTTCGGACGGAAATTGC4550.1261624972965179No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTTGTACAGCAACAGGAGGGA4520.12533065665500243No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTTGATGTTGGATCTTC4520.12533065665500243No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT4510.1250533764411639No Hit
GCCTTAGAGTCTCCTGAGCATTGTACACCTCACCATACTGCACTCAGGCA4480.12422153579964841No Hit
TCCTCAGGCCGTGCAGTGGAACTCCACAACTTTCCACCAAACTCTGCAAG4480.12422153579964841No Hit
GTCTATAAGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATAC4470.1239442555858099No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACGACC4410.1222805743027789No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA4380.12144873366126341No Hit
GGCAAGGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCC4320.1197850523782324No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4320.1197850523782324No Hit
TCTGTGGAGTTACTCTCGTTTTTGCCTGCTGACTTCTTTCCTTCAGTACG4190.11618040959833187No Hit
GTATGGATCGGCAGAGGAGCCGAAAAGGTTCCACGCATGCGCTGATGGCC4180.11590312938449339No Hit
GTGAATCCCAGCCCGAATGCTCCAGCCCCTACCTTGTTGGCGTCTGGCCA4180.11590312938449339No Hit
ATACTGCACTCAGGCAAGCAATTTTGTGCTGGGGGGAACTAATGACTCTA4110.11396216788762388No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC4110.11396216788762388No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGTTCTTCTGCGAC4110.11396216788762388No Hit
CTTTCAGGCTCCTCTGCCGATCCATACTGCGGAACTCCTAGCCGCTTGTT4080.11313032724610839No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTTTTCAAGCCTCCAAGCTGTGC4070.11285304703226988No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG4070.11285304703226988No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT4060.11257576681843137No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA4050.11229848660459286No Hit
CCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAG4030.11174392617691586No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG4020.11146664596307736No Hit
GTGCAGTGGAACTCCACAACTTTCCACCAAACTCTGCAAGATCCCAGAGT4010.11118936574923888No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC4000.11091208553540037No Hit
TACATGCATACAAGGGCTTTAAAGCAGGATAACCACATTGTGTAAAAGGG3990.11063480532156186No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGA3990.11063480532156186No Hit
CCACCGCACGGCCTGAGGATGAGTTTGTCTCAAAGGTGGAGACAGCGGAG3950.10952568446620785No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG3940.10924840425236937No Hit
ATACAGCATGGGGCAGAATCTTTCCACCAGCAATCCTCTGGGATTCTTTC3910.10841656361085386No Hit
ATGTGGTATTGGGGGCCAAGTCTGTGTCGCATCTTGAGTCCCTTTTTACC3880.10758472296933834No Hit
CTTTTCGGCTCCTCTGCCGATCCATACTGCGGAACTCCTAGCCGCTTGTT3880.10758472296933834No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC3880.10758472296933834No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC3870.10730744275549986No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA3870.10730744275549986No Hit
GAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAATACTAAC3840.10647560211398437No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC3830.10619832190014586No Hit
GTAGAAGAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT3830.10619832190014586No Hit
CCTTTCAGGCTCCTCTGCCGATCCATACTGCGGAACTCCTAGCCGCTTGT3820.10592104168630735No Hit
ATCCAACATCAAGGGACCTAGTAGTCAGTTATGTCAACACTAATATGGGC3820.10592104168630735No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT3790.10508920104479184No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGTTCT3780.10481192083095334No Hit
GTGAAAGGCCTGTATCTCACTGCTGGTGGCTCCAGTTCAGGAACAGTAAA3760.10425736040327635No Hit
CCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAAGAGTCCTCTTATGT3760.10425736040327635No Hit
CCATATTCTTGGGAACAAGAGATACAGCATGGGGCAGAATCTTTCCACCA3740.10370279997559934No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT3730.10342551976176084No Hit
ACTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAG3720.10314823954792234No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAAAGCCCTTGTATGCCTGTATT3720.10314823954792234No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG3630.10065271762337584No Hit
AAACAACACATAGCGCCTCATTTTGTGGGTCACCATATTCTTGGGAACAA3630.10065271762337584No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)