FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences360646
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT19310.5354280929221453No Hit
GTATCTCACTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA18730.5193458405195122No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA18310.5077000715382951No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG18100.5018771870476867No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC16680.4625033966826195No Hit
GGAATATGATAAAACGCCGCAGACACATCCAGCGATAACCAGGACAAGTT16150.447807545349179No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAACAAC16020.44420290256927847No Hit
CCTCCAGCTTATAGACCATCAAATGCCCCTATCTTATCAACACTTCCGGA15590.4322798533742229No Hit
CTCTATGGACGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC14390.39900622771360283No Hit
GTCCCGTGCTGGTCGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC10980.304453674794674No Hit
GAGACAAACTCATCCTCAGGCCGTGCAGTGGAACTCCACAACTTTCCACC10620.29447158709648796No Hit
ATATAGGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT9470.2625843625050604No Hit
ATCATCTTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT9080.2517704341653588No Hit
GGGAAATACAGGCCTTTCACTCTGGGATCTTGCAGAGTTTGGTGGAAAGT8720.2417883464671728No Hit
ATGTCATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAAT8340.23125169834130976No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG8260.22903345663060176No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA8170.22653793470605524No Hit
GTCTTTTGGGTTTTGCTGCCCCTTTTACACAATGTGGTTATCCTGCTTTA7980.22126961064312373No Hit
GCCGAAAAGGTTCCACGCATGCGCTGATGGCCCATGACCAAGCCCCAGCC7670.2126739240141302No Hit
ATGGACGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCCTCAT7650.2121193635864532No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG7530.2087920010203912No Hit
ATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGACT7310.20269183631594417No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT7200.1996417539637207No Hit
CTACAAACCCTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC7030.19492799032846614No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC7020.19465071011462765No Hit
TCATCTTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTT6930.19215518819008112No Hit
GTGAGATACAGGCCTTTCACTCTGGGATCTTGCAGAGTTTGGTGGAAAGT6880.19076878712088863No Hit
CCCAACAGACCTACAATACGTTGACAGACTTTCCAATCAATAGGCCTGTT6800.18855054541018063No Hit
GTTTCTCTCAAAGGTGGAGACAGCGGAGTAGGCTGCCTTCCTGACTGGCG6790.18827326519634213No Hit
GCAGTGAGATACAGGCCTTTCACTCTGGGATCTTGCAGAGTTTGGTGGAA6720.18633230369947262No Hit
ACTTTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGACATGGACAT6630.18383678177492613No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT6490.1799548587811871No Hit
GCCTGAAAGGTTCCACGCATGCGCTGATGGCCCATGACCAAGCCCCAGCC6460.1791230181396716No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT6420.1780138972843176No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT6380.17690477642896357No Hit
CTCTTATATAATATACCCGCCGTCCATAGAGTGTGTAAATAATGTCTGGT6190.17163645236603206No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG6160.17080461172451655No Hit
GGGACTCACCCCACCGCACGGAGGCCTTTTGGGGTGGAGCCCTCAGGCTC6080.16858637001380855No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5860.16248620530936153No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT5800.16082252402633054No Hit
GAGTAGGCTGCCTTCCTGACTGGCGATTGGTAGAGGCAGGAGGCGGATTT5790.16054524381249202No Hit
CCTTTCAGGCTCCTCTGCCGATCCATACTGCGGAACTCCTAGCCGCTTGT5760.15971340317097654No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA5690.15777244167410703No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATA5670.15721788124643002No Hit
GTGTAAAAGGGGCAGCAAAACCCAACAGACCTACAATACGTTGACAGACT5660.15694060103259153No Hit
GTATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTACACCTCACCATACTG5660.15694060103259153No Hit
CCCATAACATCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCATC5550.15389051868036802No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA5530.15333595825269103No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC5520.1530586780388525No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT5470.15167227696965999No Hit
CTTTAGGACATGGACATCGACCCTTATAAAGAATTTGGAGCTTCTGTGGA5450.151117716541983No Hit
CTATGTATCCCTCCTGTTGCTGTACAAAACCTTCGGACGGAAATTGCACC5380.1491767550451135No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5350.14834491440359798No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC5240.14529483205137447No Hit
CTTTCAGGCTCCTCTGCCGATCCATACTGCGGAACTCCTAGCCGCTTGTT5080.14085834862995847No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA5010.13891738713308896No Hit
GCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAAC4960.13753098606389647No Hit
CTCTATGACTGTTGCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA4940.13697642563621945No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC4940.13697642563621945No Hit
TCCTCAGGCCGTGCAGTGGAACTCCACAACTTTCCACCAAACTCTGCAAG4890.13559002456702696No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4860.13475818392551145No Hit
TTGTACACCTCACCATACTGCACTCAGGCAAGCAATTTTGTGCTGGGGGG4830.13392634328399594No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC4780.13253994221480342No Hit
CTTTTCGGCTCCTCTGCCGATCCATACTGCGGAACTCCTAGCCGCTTGTT4780.13253994221480342No Hit
TCACCATACTGCACTCAGGCAAGCAATTTTGTGCTGGGGGGAACTAATGA4690.13004442029025692No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC4670.1294898598625799No Hit
CTGTTGCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACAATAGTTGC4660.12921257964874144No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4620.12810345879338741No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT4550.1261624972965179No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4550.1261624972965179No Hit
GCCTTAGAGTCTCCTGAGCATTGTACACCTCACCATACTGCACTCAGGCA4530.12560793686884092No Hit
GTCTATAAGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATAC4530.12560793686884092No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACAAAACCTTCGGACGGAAATTGC4460.12366697537197141No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCC4450.1233896951581329No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT4420.12255785451661741No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4420.12255785451661741No Hit
ATGTGGTATTGGGGGCCAAGTCTGTGTCGCATCTTGAGTCCCTTTTTACC4400.1220032940889404No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAAAGCCCTTGTATGCCTGTATT4330.12006233259207089No Hit
ATGCCTGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAG4330.12006233259207089No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4330.12006233259207089No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACGACC4320.1197850523782324No Hit
GTGCAGTGGAACTCCACAACTTTCCACCAAACTCTGCAAGATCCCAGAGT4310.1195077721643939No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTTTTCAAGCCTCCAAGCTGTGC4310.1195077721643939No Hit
GAATCTTTCCACCAGCAATCCTCTGGGATTCTTTCCCGACCACCAGTTGG4270.11839865130903988No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4260.11812137109520138No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA4260.11812137109520138No Hit
GTCTTGGTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGT4150.1150712887429779No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTTGATGTTGGATCTTC4150.1150712887429779No Hit
TCTGTGGAGTTACTCTCGTTTTTGCCTGCTGACTTCTTTCCTTCAGTACG4140.11479400852913937No Hit
ATACTGCACTCAGGCAAGCAATTTTGTGCTGGGGGGAACTAATGACTCTA4130.11451672831530088No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCG4110.11396216788762388No Hit
GTGAATCCCAGCCCGAATGCTCCAGCCCCTACCTTGTTGGCGTCTGGCCA4040.11202120639075437No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC3980.11035752510772336No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC3970.11008024489388486No Hit
CCATATTCTTGGGAACAAGAGATACAGCATGGGGCAGAATCTTTCCACCA3940.10924840425236937No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT3920.10869384382469235No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTTGTACAGCAACAGGAGGGA3900.10813928339701535No Hit
ATACAGCATGGGGCAGAATCTTTCCACCAGCAATCCTCTGGGATTCTTTC3880.10758472296933834No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG3880.10758472296933834No Hit
CCACCGCACGGCCTGAGGATGAGTTTGTCTCAAAGGTGGAGACAGCGGAG3870.10730744275549986No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT3860.10703016254166135No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGTTCTTCTGCGAC3840.10647560211398437No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGA3830.10619832190014586No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG3780.10481192083095334No Hit
ATCCAACATCAAGGGACCTAGTAGTCAGTTATGTCAACACTAATATGGGC3760.10425736040327635No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC3740.10370279997559934No Hit
CTTATATAATATACCCGCCGTCCATAGAGTGTGTAAATAATGTCTGGTTT3730.10342551976176084No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGTTCT3720.10314823954792234No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACA3720.10314823954792234No Hit
GTAGAAGAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT3720.10314823954792234No Hit
GGCAAGGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCC3660.10148455826489133No Hit
GTATGGATCGGCAGAGGAGCCGAAAAGGTTCCACGCATGCGCTGATGGCC3650.10120727805105283No Hit
TTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGACTA3650.10120727805105283No Hit
AAACAACACATAGCGCCTCATTTTGTGGGTCACCATATTCTTGGGAACAA3640.10092999783721432No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT3640.10092999783721432No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG3630.10065271762337584No Hit
CCTTTTCGGCTCCTCTGCCGATCCATACTGCGGAACTCCTAGCCGCTTGT3620.10037543740953733No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)