FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422164
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC22020.5215982414417145No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACTACACATAGCGCC15350.3636027704872988No Hit
CCTCCAGCTTATAGACCTTCAAATGCCCCTATCTTATCAACACTTCCGGA15200.3600496489515923No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA15180.35957589941349805No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT13890.32901905420642213No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC11620.2752484816327304No Hit
TTACTGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGG9890.23426914658758205No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC9840.23308477274234657No Hit
ATATAAGAGAGAAACTACACATAGCGCCTCATTTTGCGGGTCACCATATT9490.2247941558256981No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG9420.22313603244236835No Hit
CATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGAC9370.22195165859713287No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCAACCAATCGCCAGTC9330.22100415952094446No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG9310.22053040998285026No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC9220.21839853706142634No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACGGAGAGTCCAA9160.21697728844714376No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT8650.20489667522574168No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8390.19873793123051706No Hit
CGGTAGTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTC8380.19850105646146993No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA8340.19755355738528155No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG7980.18902606569958594No Hit
GAACAGATAACTCTGTTGTTCTCTCCCGCAAATATACATCGTTTCCATGG7570.17931420016865485No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC7550.17884045063056064No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC7490.17741920201627803No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7380.1748135795567599No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC7380.1748135795567599No Hit
GTATGGATCGGCAGAGGAGCCTGAAAGGTTCCACGCATGCGCTGATGGCC7370.17457670478771284No Hit
CTGTCTGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGC7240.17149733279010052No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT7230.17126045802105344No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT7150.16936545986867663No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT7130.16889171033058242No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC7120.16865483556153532No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC7030.16652296264011143No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG6990.16557546356392303No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC6970.16510171402582882No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAGAACCTAGCAGG6840.16202234202821653No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT6720.1591798447996513No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA6720.1591798447996513No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6660.15775859618536872No Hit
TTATATAAGAGAGAAACTACACATAGCGCCTCATTTTGCGGGTCACCATA6630.15704797187822742No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC6570.1556267232639448No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA6550.15515297372585063No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG6280.1487573549615789No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGAGGGGTAGGCTGCCT6230.1475729811163434No Hit
GCTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG6170.14615173250206082No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGAGTTAAAGA6110.1447304838877782No Hit
AAACTACACATAGCGCCTCATTTTGCGGGTCACCATATTCTTGGGAACAA6090.14425673434968403No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC6090.14425673434968403No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG6060.14354611004254272No Hit
TATCCGTCCCGAGAATGTTTGCTCCAGACCTGCTGCGAGCAAAACAAGCG5900.1397561137377891No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT5800.1373873660473181No Hit
GAACCACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCAC5790.137150491278271No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5790.137150491278271No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC5710.13525549312589422No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5700.1350186183568471No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAAATACAGGCCTTT5680.1345448688187529No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA5660.1340711192806587No Hit
TTGTCAGGGTTTAAATGTATACCCAGAGACAAAAGAAAATTGGTAACAGC5630.1333604949735174No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC5630.1333604949735174No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5620.1331236202044703No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5610.1328867454354232No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG5600.13264987066637612No Hit
CCGATAATGTTTGCTCCAGACCTGCTGCGAGCAAAACAAGCGGCTAGGAG5500.1302811229759051No Hit
ATGCTGTACAGACTTCGCCCCCAATACCACATCATCCATATAACTGAAAG5470.1295704986687638No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA5470.1295704986687638No Hit
GTACCGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC5450.1290967491306696No Hit
CATTTAAACCCTAACAAAACAAAACGATGGGGTTACTCTTTACATTTCAT5370.12720175097829278No Hit
CTGTTGTTCTCTCCCGCAAATATACATCGTTTCCATGGCTGCTAGGCTGT5350.12672800144019858No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA5300.1255436275949631No Hit
GTACAGACTTCGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5220.1236486294425863No Hit
GTTTAAATGTATACCCAGAGACAAAAGAAAATTGGTAACAGCGGTAAAAA5210.12341175467353918No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTGCTGACTTCTTTCCTTCAGTACG5200.1231748799044921No Hit
GGCAACTATTGTGGTTTCACATTTCTTGTCTCACTTTTGGAAGAGAAACG5200.1231748799044921No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAATCTAGTCTCCTCC5170.12246425559735079No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGACTCTA5100.12080613221402109No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC5070.12009550790687978No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG5060.11985863313783268No Hit
TCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGA5020.11891113406164429No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCC4970.1177267602164088No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT4970.1177267602164088No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCG4920.11654238637117328No Hit
TCCTCACAATACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTT4890.11583176206403198No Hit
CCCCAAAAGGCCTCCGTGCGGTGGGGTGAATCCCAGCCCGAATGCTCCAG4890.11583176206403198No Hit
CCCTAACAAAACAAAACGATGGGGTTACTCTTTACATTTCATGGGCTATG4880.11559488729498489No Hit
ACTATATGTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATA4860.11512113775689067No Hit
TGTATACCCAGAGACAAAAGAAAATTGGTAACAGCGGTAAAAAGGGACTC4850.11488426298784359No Hit
ACTACACATAGCGCCTCATTTTGCGGGTCACCATATTCTTGGGAACAAGA4830.1144105134497494No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4820.11417363868070228No Hit
TTGTTAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACGAGCAGG4790.11346301437356097No Hit
ATAATTGCCTGAACTTTAGGCCCATATGAGTGTTAACATAACTGACTACT4770.11298926483546679No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT4730.11204176575927839No Hit
GGATATTGGATCTTCCAAATTACCACCCACCCAGGTGGCTAGAGTCATTA4710.11156801622118417No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAGAAC4700.11133114145213709No Hit
TATCTGTTCCGATAATGTTTGCTCCAGACCTGCTGCGAGCAAAACAAGCG4660.11038364237594869No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA4640.10990989283785448No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG4600.10896239376166608No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT4580.10848864422357188No Hit
GCTCTATATCGGGATGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCA4570.10825176945452478No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAG4540.10754114514738347No Hit
CCCCTATCTTATCAACACTTCCGGAGACTTGTGTTGTTAGACGACGAGGC4470.10588302176405377No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG4460.10564614699500668No Hit
GACTTCGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTG4450.10540927222595958No Hit
GTAGAAGAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT4440.10517239745691247No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG4420.10469864791881828No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA4370.1035142740735828No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4340.10280364976644149No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA4300.10185615069025308No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4290.10161927592120597No Hit
GAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAATACTAAC4270.10114552638311178No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC4270.10114552638311178No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA4260.10090865161406466No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)