FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422164
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC21260.503595758994135No Hit
CCTCCAGCTTATAGACCTTCAAATGCCCCTATCTTATCAACACTTCCGGA16890.4000814849205522No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA14910.35318028064922635No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACTACACATAGCGCC14720.3486796600373315No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT13370.31670156621597295No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG9820.2326110232042524No Hit
CATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGAC9790.2319003988971111No Hit
GAACAGATAACTCTGTTGTTCTCTCCCGCAAATATACATCGTTTCCATGG9430.22337290721141548No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG9130.21626666414000248No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC9120.21602978937095538No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC9100.21555603983286117No Hit
ATATAAGAGAGAAACTACACATAGCGCCTCATTTTGCGGGTCACCATATT9030.21389791644953146No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA8860.20987104537573076No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCAACCAATCGCCAGTC8540.20229105276622356No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT8510.20158042845908225No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC8390.19873793123051706No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC7970.18878919093053884No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT7740.18334107124245555No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7580.17955107493770195No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG7490.17741920201627803No Hit
CGGTAGTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTC7480.17718232724723093No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7320.17339233094247733No Hit
GTATGGATCGGCAGAGGAGCCTGAAAGGTTCCACGCATGCGCTGATGGCC7300.17291858140438313No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACGGAGAGTCCAA7270.17220795709724182No Hit
CTGTCTGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGC7250.17173420755914764No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC7150.16936545986867663No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG7140.16912858509962952No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC7140.16912858509962952No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA7010.16604921310201723No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC6990.16557546356392303No Hit
TTACTGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGG6930.16415421494964041No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA6900.16344359064249914No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAGAACCTAGCAGG6740.1596535943377455No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG6730.15941671956869843No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT6690.15846922049251003No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT6570.1556267232639448No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC6550.15515297372585063No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT6540.1549160989568035No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6490.15373172511156802No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGAGTTAAAGA6470.15325797557347381No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC6410.15183672695919123No Hit
CTGTTGTTCTCTCCCGCAAATATACATCGTTTCCATGGCTGCTAGGCTGT6330.14994172880681442No Hit
TTATATAAGAGAGAAACTACACATAGCGCCTCATTTTGCGGGTCACCATA6280.1487573549615789No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC6120.1449673586568253No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6110.1447304838877782No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT6010.14236173619730721No Hit
GAACCACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCAC5980.1416511118901659No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA5950.1409404875830246No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG5910.1399929885068362No Hit
AAACTACACATAGCGCCTCATTTTGCGGGTCACCATATTCTTGGGAACAA5800.1373873660473181No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5710.13525549312589422No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGAGGGGTAGGCTGCCT5690.1347817435878No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC5670.1343079940497058No Hit
GTACAGACTTCGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5670.1343079940497058No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5630.1333604949735174No Hit
GTTTAAATGTATACCCAGAGACAAAAGAAAATTGGTAACAGCGGTAAAAA5590.132412995897329No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5570.13193924635923482No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAAATACAGGCCTTT5500.1302811229759051No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC5480.1298073734378109No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAGAAC5460.1293336238997167No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTGCTGACTTCTTTCCTTCAGTACG5440.1288598743616225No Hit
GCTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG5440.1288598743616225No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA5400.1279123752854341No Hit
TTGTCAGGGTTTAAATGTATACCCAGAGACAAAAGAAAATTGGTAACAGC5380.12743862574733988No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT5350.12672800144019858No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG5240.12412237898068049No Hit
GTACCGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC5230.1238855042116334No Hit
GTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCTTCTCGGGGCCG5160.12222738082830369No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC5130.1215167565211624No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCG5100.12080613221402109No Hit
TATCCGTCCCGAGAATGTTTGCTCCAGACCTGCTGCGAGCAAAACAAGCG5010.1186742592925972No Hit
ACTATATGTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATA4990.11820050975450298No Hit
CCCTAACAAAACAAAACGATGGGGTTACTCTTTACATTTCATGGGCTATG4990.11820050975450298No Hit
ATAATTGCCTGAACTTTAGGCCCATATGAGTGTTAACATAACTGACTACT4980.1179636349854559No Hit
CCGATAATGTTTGCTCCAGACCTGCTGCGAGCAAAACAAGCGGCTAGGAG4920.11654238637117328No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCC4920.11654238637117328No Hit
GCTCTATATCGGGATGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCA4870.11535801252593779No Hit
GCTTTAATGCCCTTGTATGCATGTATTCAATCTAAGCAGGCTTTCACTTT4810.11393676391165519No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA4810.11393676391165519No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT4790.11346301437356097No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4760.1127523900664197No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGACTCTA4750.11251551529737258No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4750.11251551529737258No Hit
ATGCTGTACAGACTTCGCCCCCAATACCACATCATCCATATAACTGAAAG4750.11251551529737258No Hit
CCCCAAAAGGCCTCCGTGCGGTGGGGTGAATCCCAGCCCGAATGCTCCAG4700.11133114145213709No Hit
CATTTAAACCCTAACAAAACAAAACGATGGGGTTACTCTTTACATTTCAT4690.11109426668309No Hit
GGATATTGGATCTTCCAAATTACCACCCACCCAGGTGGCTAGAGTCATTA4690.11109426668309No Hit
TCCTCACAATACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTT4650.11014676760690158No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4590.10872551899261898No Hit
TCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGA4590.10872551899261898No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG4530.10730427037833638No Hit
TATCTGTTCCGATAATGTTTGCTCCAGACCTGCTGCGAGCAAAACAAGCG4520.10706739560928928No Hit
GGCAACTATTGTGGTTTCACATTTCTTGTCTCACTTTTGGAAGAGAAACG4500.10659364607119508No Hit
GTAGAAGAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT4490.10635677130214798No Hit
CCCCTATCTTATCAACACTTCCGGAGACTTGTGTTGTTAGACGACGAGGC4480.10611989653310089No Hit
TGTATACCCAGAGACAAAAGAAAATTGGTAACAGCGGTAAAAAGGGACTC4410.10446177314977119No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA4400.10422489838072407No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG4400.10422489838072407No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGGCATGGACATTGA4390.10398802361167697No Hit
GACTTCGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTG4380.10375114884262988No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC4380.10375114884262988No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC4380.10375114884262988No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4360.10327739930453567No Hit
GTCTATAAGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATAC4330.10256677499739437No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGGCATG4320.10232990022834727No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAG4310.10209302545930019No Hit
GGTTTGGTACAGCAACAGGAGGGATACATAGAGGTTCCTTGAGCAGGAGT4300.10185615069025308No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG4300.10185615069025308No Hit
ATCCTGGGCTTTCGGAAAATTCCTATGGGAGTGGGCCTCAGCCCGTTTCT4280.10138240115215888No Hit
GGGTATACATTTAAACCCTAACAAAACAAAACGATGGGGTTACTCTTTAC4270.10114552638311178No Hit
CTTCTAGATACCGCCTCAGCTCTATATCGGGATGCCTTAGAGTCTCCTGA4250.10067177684501757No Hit
ACTACACATAGCGCCTCATTTTGCGGGTCACCATATTCTTGGGAACAAGA4250.10067177684501757No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAATCTAGTCTCCTCC4240.10043490207597049No Hit
GTCTTGGTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGAGT4230.10019802730692338No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)