FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490494

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490494
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences524298
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC34600.6599300397865336No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA28630.5460634982395508No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA24590.46900808318933124No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACACAGCGCC22250.4243769764523229No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC18190.3469400989513597No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG16740.3192840712724443No Hit
CACTCATATGGGCCTAAAGTTCCGGCAACTATTGTGGTTTCACATTTCTT16400.31279920960980206No Hit
ATGCTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG14910.2843802570293993No Hit
CACTAATATGGGCCTAAAGTTCCGGCAACTATTGTGGTTTCACATTTCTT14490.27636954556378246No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT14340.273508577183205No Hit
GTATTCCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA12150.23173843882677408No Hit
ATATAAGAGAGAAACAACACACAGCGCCTCATTTTGTGGGTCACCATATT10950.20885069178215443No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG10700.20408241114785863No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA10590.20198436766876854No Hit
ATGTAAGACCTTGGGCAAGAATCGGTGGGCGTTCACGGTGGTCTCCATGC10560.20141217399265304No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTGGATACTGGATCTTC10510.20045851786579388No Hit
GTTATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC10250.1954995060061263No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9570.18252978268084183No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG9560.18233905145547No Hit
GTGAAAGGCCTGTATTCCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAA9480.18081320165249534No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTT9340.1781429644972897No Hit
TTATATAAGAGAGAAACAACACACAGCGCCTCATTTTGTGGGTCACCATA9270.17680784591968687No Hit
GCCTAGCAGCCATGGATACGATGTATATTTGCGGGAGAGAACAACAGAGT9190.17528199611671225No Hit
GGATACTGGATCTTCCAAATTACCACCCACCCAGGTAGCTAGAGTCATTA9150.17451907121522492No Hit
GTCCCGTGCTGGTAGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC9140.1743283399898531No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT8950.1707044467077883No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTAACCAATTTTCTTTTGTC8900.16975079058092918No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT8870.16917859690481368No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG8830.16841567200332636No Hit
CTCTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA8810.1680342095525827No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATCGGTGGGCGTTC8770.16727128465109536No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG8670.16536397239737707No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC8620.16441031627051791No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG8530.16269373524217143No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG8430.16078642298845314No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT8360.15945130441085031No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC8330.15887911073473482No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA8260.15754399215713202No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC8120.15487375500192638No Hit
GTATGGATCGGCAGAGGAGCCCGAAAGGTTCCACGCATGCGCTGATGGCC7840.1495332806915151No Hit
GGATAAGGGTATTAAACCTTATTACCCAGAACATCTAGTTAATCATTACT7820.14915181824077145No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7820.14915181824077145No Hit
GGCCTCTCACTCTGGGATCTTGCAGAGTTTGGTGGAAGGTTGTGGAGTTC7790.14857962456465595No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT7730.147435237212425No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA7660.14610011863482217No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA7580.14457426883184754No Hit
GAATCGGTGGGCGTTCACGGTGGTCTCCATGCGACGTGCAGAGGTGAAGC7490.14285768780350105No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7420.14152256922589826No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACTACC7310.1394245257468081No Hit
GTATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCTCCTCACCATACGG7310.1394245257468081No Hit
TCCAATATCCAGGGACCTAGTAGTCAGTTATGTCAACACTCATATGGGCC7270.1386616008453208No Hit
CAGACACATCCAGCGATAACCAGGACAAGTTGGAGGACAGGAGGTTGGTG7220.13770794471846165No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT7180.13694501981697432No Hit
GTATCTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA7140.136182094915487No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT7110.1356099012393715No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG7060.13465624511251234No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT7020.133893320211025No Hit
ATGTATCTGCGGCGTTTTATCATCTTCCTCTTCATCCTGCTGCTATGCCT7000.13351185776028135No Hit
ACAACACACAGCGCCTCATTTTGTGGGTCACCATATTCTTGGGAACAAAA6960.13274893285879405No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA6920.13198600795730672No Hit
GTATCGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6910.1317952767319349No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA6900.13160454550656306No Hit
TCTAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGAGC6840.13046015815433207No Hit
GCTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG6730.12836211467524195No Hit
ATATGAGTGTTGACATAACTGACTACTAGGTCCCTGGATATTGGATCTTC6670.12721772732301095No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCTCCTCACCATACGGCACTCAGGCA6570.12531041506929266No Hit
AATATACATCGTATCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG6450.1230216403648307No Hit
GATGCGATACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAA6440.12283090913945886No Hit
GCCTGAGGATGAGTGTCTCTCAAAGGTGGAGACAGCGGGGTAGGCTGCCT6420.1224494466887152No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA6390.1218772530125997No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG6360.12130505933648421No Hit
GGGGAATACAGGCCTTTCACTCTGGGATCTTGCAGAGTTTGGTGGAAGGT6350.12111432811111238No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT6340.12092359688574054No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC6240.11901628463202225No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG6240.11901628463202225No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT6230.11882555340665042No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATAA6180.11787189727979126No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA6090.11615531625144479No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC6040.11520166012458563No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC6000.1144387352230983No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5920.11291288542012368No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGCTT5880.11214996051863635No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5880.11214996051863635No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA5850.11157776684252085No Hit
CTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACAAT5830.11119630439177719No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT5810.11081484194103354No Hit
ATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATTCAGTCAAAGC5760.10986118581417438No Hit
TCCCGATACAGAGCTGAGGCGGTATCTAGAAGATCACGTACTGAAGGAAA5760.10986118581417438No Hit
CTCTGGGATCTTGCAGAGTTTGGTGGAAGGTTGTGGAGTTCCACTGCATG5760.10986118581417438No Hit
GCCTAATCATCTTTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5580.10642802375748144No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG5560.10604656130673777No Hit
CCATATTCTTGGGAACAAAAGCTACAGCATGGGGCAGAATCTTTCCACCA5410.10318559292616031No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC5380.10261339925004481No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5370.10242266802467298No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCC5340.10185047434855748No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC5330.10165974312318565No Hit
GTATGCATGTATTCAGTCAAAGCAGGCTTTTACTTTCTCGCCAACTTACA5300.10108754944707019No Hit
TCCTAATACAAAGATCATTAACCTAATCTCCTCCCCCGACTCCTCCCAGT5300.10108754944707019No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT5260.10032462454558286No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)