FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences524298
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC32870.626933537797207No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA28470.5430117986336015No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA22570.43048037566422154No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACACAGCGCC21990.4194179645926553No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG18370.35037326100805266No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC16070.30650507917253167No Hit
CACTCATATGGGCCTAAAGTTCCGGCAACTATTGTGGTTTCACATTTCTT14570.2778953953667571No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT14260.2719827273802303No Hit
GTATTCCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA13370.2550076483221374No Hit
CACTAATATGGGCCTAAAGTTCCGGCAACTATTGTGGTTTCACATTTCTT13160.251002292589329No Hit
ATGCTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG12640.24108426886999376No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG10680.20370094869711497No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG10260.19569023723149814No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTGGATACTGGATCTTC10260.19569023723149814No Hit
GTGAAAGGCCTGTATTCCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAA10220.19492731233001082No Hit
ATATAAGAGAGAAACAACACACAGCGCCTCATTTTGTGGGTCACCATATT10190.19435511865389532No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9570.18252978268084183No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT9450.18024100797637985No Hit
GTTATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC9360.17852442694803336No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG9310.1775707708211742No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTAACCAATTTTCTTTTGTC9100.17356541508836576No Hit
GCCTAGCAGCCATGGATACGATGTATATTTGCGGGAGAGAACAACAGAGT9010.1718488340600193No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA8810.1680342095525827No Hit
CTCTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA8760.16708055342572356No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT8640.16479177872126158No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC8570.16345666014365876No Hit
TTATATAAGAGAGAAACAACACACAGCGCCTCATTTTGTGGGTCACCATA8480.16174007911531227No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC8470.16154934788994044No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG8460.1613586166645686No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT8370.15964203563622215No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTT8160.1556366799034137No Hit
AATATACATCGTATCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG8160.1556366799034137No Hit
GGATAAGGGTATTAAACCTTATTACCCAGAACATCTAGTTAATCATTACT7940.15144059294523343No Hit
GCTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG7900.1506776680437461No Hit
ATGTAAGACCTTGGGCAAGAATCGGTGGGCGTTCACGGTGGTCTCCATGC7800.1487703557900278No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT7630.14552792495870667No Hit
GTATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCTCCTCACCATACGG7580.14457426883184754No Hit
GTATCGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC7560.14419280638110388No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG7530.14362061270498838No Hit
GGATACTGGATCTTCCAAATTACCACCCACCCAGGTAGCTAGAGTCATTA7510.14323915025424472No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA7510.14323915025424472No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA7510.14323915025424472No Hit
GTCCCGTGCTGGTAGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC7500.1430484190288729No Hit
ATGTATCTGCGGCGTTTTATCATCTTCCTCTTCATCCTGCTGCTATGCCT7470.1424762253527574No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA7420.14152256922589826No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7400.1411411067751546No Hit
GTATCTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA7370.1405689130990391No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT7280.13885233207069264No Hit
GTATGGATCGGCAGAGGAGCCCGAAAGGTTCCACGCATGCGCTGATGGCC7230.13789867594383348No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7200.137326482267718No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA6960.13274893285879405No Hit
TCCAATATCCAGGGACCTAGTAGTCAGTTATGTCAACACTCATATGGGCC6800.12969723325284474No Hit
ACAACACACAGCGCCTCATTTTGTGGGTCACCATATTCTTGGGAACAAAA6730.12836211467524195No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA6690.12759918977375462No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG6650.1268362648722673No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATCGGTGGGCGTTC6640.12664553364689546No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG6610.12607333997078No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA6570.12531041506929266No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGGCATG6530.12454749016780534No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACTACC6420.1224494466887152No Hit
GCCTAATCATCTTTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6360.12130505933648421No Hit
TCTAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGAGC6340.12092359688574054No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT6330.12073286566036871No Hit
GGCCTCTCACTCTGGGATCTTGCAGAGTTTGGTGGAAGGTTGTGGAGTTC6130.11691824115293212No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGGCATGGACATTGA6120.11672750992756029No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT6120.11672750992756029No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC6120.11672750992756029No Hit
ATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATTCAGTCAAAGC6070.11577385380070113No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG6070.11577385380070113No Hit
GCCTGAGGATGAGTGTCTCTCAAAGGTGGAGACAGCGGGGTAGGCTGCCT6040.11520166012458563No Hit
ATATGAGTGTTGACATAACTGACTACTAGGTCCCTGGATATTGGATCTTC5960.113675810321611No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC5950.11348507909623917No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5930.11310361664549551No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCTCCTCACCATACGGCACTCAGGCA5860.11176849806789269No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5850.11157776684252085No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT5700.10871679846194339No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG5650.10776314233508426No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATAA5650.10776314233508426No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT5630.1073816798843406No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5580.10642802375748144No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA5570.1062372925321096No Hit
GAATCTTTCCACCAGCAATCCTCTGGGATTCTTCCCCGACCACCAGTTGG5450.10394851782764764No Hit
GGGGAATACAGGCCTTTCACTCTGGGATCTTGCAGAGTTTGGTGGAAGGT5450.10394851782764764No Hit
CAGACACATCCAGCGATAACCAGGACAAGTTGGAGGACAGGAGGTTGGTG5420.10337632415153214No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA5400.10299486170078848No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT5390.10280413047541664No Hit
CTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACAAT5370.10242266802467298No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC5370.10242266802467298No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC5310.10127828067244202No Hit
CTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAA5300.10108754944707019No Hit
GATGCGATACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAA5280.10070608699632652No Hit
CTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGA5280.10070608699632652No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)