FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490497

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490497
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences309712
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG11550.3729271064731105No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC11550.3729271064731105No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCCTATCAACACTTCCGGA8710.28122901276024176No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8300.26799090768197553No Hit
TCCATAGCGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA8140.262824817895335No Hit
CTCTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA7120.2298909955055019No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACT6600.21310120369892027No Hit
GTTATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC6420.20728935268894974No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA6290.20309190473730435No Hit
CTCTTATATAATATACCCGCCTTCCATAGCGTGTGTAAATAGTGTCTAGT6030.19469700883401353No Hit
CTTATATAATATACCCGCCTTCCATAGCGTGTGTAAATAGTGTCTAGTTT6000.19372836699901844No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT5840.18856227721237795No Hit
CTATTTACACACGCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAA5690.18371906803740248No Hit
GATCAATAATCTAGTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC5670.18307330681407244No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCATCTTTTCCTAAT5600.1808131425324172No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT5590.18049026192075218No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC5560.1795216200857571No Hit
CCCTTATCCAATGGTAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG5410.17467841091078162No Hit
GTGTAAATGTATATTAGGAAAAGATGGTGTTTTCCAATGAGGATTAAAGA5240.16918944051247609No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5200.16789791806581597No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGGTTCCCGAGATTGAGATCT5050.1630547088908405No Hit
ATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTCACGGTGGTCTCCATGC4920.15885726093919514No Hit
GGATAAGGGTATTAAACCTTACTATCCAGAACATCTAGTTAATCATTACT4850.1565970966575399No Hit
GTATTAAACCTTACTATCCAGAACATCTAGTTAATCATTACTTCCAAACT4830.15595133543420983No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4790.15465981298754972No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT4740.15304540992922458No Hit
ACACATAGCGCCTCATTTTGTGGGTCACCATATTCTTGGGAACAAGATCT4710.1520767680942295No Hit
GTTTAATACCCTTATCCAATGGTAAATATTTGGTAACCTTTGGATAAAAC4700.15175388748256446No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTACTCTCTGAATTTTAT4650.1501394844242393No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA4640.14981660381257425No Hit
TCCTCAGGCCATGCAGTGGAATTCCACAACTTTCCATCAAACTCTGCAAG4380.14142170790928346No Hit
ACATAGAGGTTCCTTGAGCAGTAGTCATGCAGGTTCGGCATGGTCCCGTG4330.13980730485095832No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGCGGGGTAGGCTGCCT4300.1388386630159632No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTC4280.13819290179263316No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATA4260.1375471405693031No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA4190.13528697628764788No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATATC4130.1333496926176577No Hit
GTTAATAGGAAGTTTTCTAAAACATTCTTTGATTTTTTGTATGATGTGTT4130.1333496926176577No Hit
ACCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGGACAACAGAGT4100.1323810507826626No Hit
CCCCTATCCTATCAACACTTCCGGAGACTACTGTTGTTAGACGACGAGGC4090.13205817017099755No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4090.13205817017099755No Hit
CTCCCGCTCCTACCTTGTTGGCGTCTGGCCAGGTGTCCTTGTTGGGATTG4070.1314124089476675No Hit
GTATGGATCGGCAGAGGAGCCTGAAAGGTTCCACGCATGCGCTGATGGCC4040.13044376711267242No Hit
CCTATTAACAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT4040.13044376711267242No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATAA4000.1291522446660123No Hit
GAATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGACGCGGTGA3890.12560055793769695No Hit
CTTCTAGATACCGCCTCAGCTCTGTTTCGGGATGCCTTAGAGTCTCCTGA3860.12463191610270186No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC3830.12366327426770678No Hit
GTCCCGTGCTGGTGGTTGAGGATCCTGGAATTAGAGGACAAACGGGCAAC3820.12334039365604174No Hit
AATATACATCGTTTCCATGGCTGCTAGGTTGTGCTGCCAACTGGATCCTG3800.12269463243271168No Hit
GGATAGGGGCATTTGGTGGTCTATAAGCTGGAGGAGTGCGAATCCACACT3790.12237175182104665No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAAATACAGGCCTCT3740.12075734876272151No Hit
GTGTGAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCGTTGACATACT3710.11978870692772639No Hit
TCTAGAGACCTAGTAGTCAGTTATGTCAATACTCATATGGGCCTAAAGTT3640.11752854264607118No Hit
GTCCAAGGAATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGAC3610.1165599008110761No Hit
ATTCCTGGGAACAAGATCTACAGCATGGGGCAGAATCTTTCCACCAGCAA3600.11623702019941107No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCTCCCATATCGTCAATCTTCTCG3600.11623702019941107No Hit
GACATACCCCATAAAATTCAGAGAGTAACCCCATCTCTTTGTTTTGTTAG3590.11591413958774605No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG3570.11526837836441597No Hit
GTATATTAGGAAAAGATGGTGTTTTCCAATGAGGATTAAAGACAGGTACA3570.11526837836441597No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC3550.11462261714108592No Hit
GGGTTATGTCATTGGATGTTATGGGTCCTTGCCACAAGAACACATCATAC3550.11462261714108592No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG3540.11429973652942087No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA3520.11365397530609081No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTC3520.11365397530609081No Hit
GACCTTTAACCTCGTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC3500.11300821408276075No Hit
ATATAATATACCCGCCTTCCATAGCGTGTGTAAATAGTGTCTAGTTTGGA3490.11268533347109572No Hit
GTTTGTAGGCCCACTCACAGCAAATGAGAAAAGAAGGTTGCAATTGATTA3480.1123624528594307No Hit
GCACGGGACCATGCCGAACCTGCATGACTACTGCTCAAGGAACCTCTATG3480.1123624528594307No Hit
GTCTTGGTGTAAATGTATATTAGGAAAAGATGGTGTTTTCCAATGAGGAT3480.1123624528594307No Hit
ATCTGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAGTG3450.1113938110244356No Hit
GGTAAATATTTGGTAACCTTTGGATAAAACCTAGCAGGCATAATCAATTG3450.1113938110244356No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGACATG3440.11107093041277058No Hit
CTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACAAG3440.11107093041277058No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA3410.11010228857777549No Hit
GTTCTGGATAGTAAGGTTTAATACCCTTATCCAATGGTAAATATTTGGTA3400.10977940796611045No Hit
CTTTAGGACATGGACATTGACCCTTATAAAGAATTTGGAGCTAGCGTGGA3380.1091336467427804No Hit
ATCCAGAACATCTAGTTAATCATTACTTCCAAACTAGACACTATTTACAC3370.10881076613111537No Hit
ATCCCAGAGTGAGAGGCCTGTATTTCCCTGCTGGTGGCTCCAGTTCAGGA3340.10784212429612025No Hit
CTACAAACTTTGCCAGCAAATCCGCCTCCTGCCTCCACCAATCGCCAGTC3310.10687348246112517No Hit
ATATGGGAGAGGCAGTAGTCGGAACAGGGTTTACTGTTCCTGAACTGGAG3300.10655060184946014No Hit
ATTCTTTGCTGGGGGGAATTAATGACTCTGGCTACCTGGGTGGGTGGTAA3300.10655060184946014No Hit
GTACAGCACCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC3240.10461331817946996No Hit
AGCGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCGGTACG3220.1039675569561399No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAG3210.10364467634447487No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA3200.10332179573280985No Hit
CACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACTCACAGC3200.10332179573280985No Hit
ATGTATATTTGCGGGAGAGGACAACAGAGTTATCAGTCCCGATAATTTTT3190.10299891512114481No Hit
CTCCGAAAGACACCAAATACTCTATAACTGTTTCTCTTCCAAAAGTGAGA3180.10267603450947978No Hit
GACCAGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGATATTGTTTACAC3160.10203027328614972No Hit
CTTATAAAGAATTTGGAGCTAGCGTGGAGTTACTCTCGTTTTTGCCTTCT3130.10106163145115461No Hit
GTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCTTCTCGGGGTCG3120.10073875083948958No Hit
GTATTGACATAACTGACTACTAGGTCTCTAGATGTTTGATCTTCCAAATT3110.10041587022782456No Hit
CGCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC3110.10041587022782456No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)