FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490498

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490498
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465887
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA24270.5209417734343307No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC21890.46985642441192815No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAATACTTCCGGA18080.3880769371113596No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAACAAC17010.3651099944836409No Hit
ATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT15460.3318401243219922No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC14820.3181028876100857No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC14680.3150978670793561No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA13730.2947066563351199No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG13460.2889112595972843No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT13010.27925226503422507No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTGCATTTCAT11960.25671461105375337No Hit
GTTATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC11940.2562853224065063No Hit
TTATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATA11580.24855812675605887No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAAACTTCCACCAAAATCTGCAAG11320.24297737434184682No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTT10910.23417695707328173No Hit
ATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACCTG10660.22881084898269322No Hit
CTACACACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC10630.2281669160118226No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC10080.21636147821252794No Hit
ATGTTTCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACCTG10060.21593218956528085No Hit
CTCTATAACTGTTTGTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA9990.21442967929991608No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA9880.21206859174005716No Hit
CCCTTATCCAATGGTAAATATTTGGTAACATTTGGATAAAACCTAGCAGG9540.2047706847368568No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT9430.20240959717699783No Hit
GCCTAGCAGCCATGGATACGATGTATATTTGCGGGAGAGAACAACAGAGT9430.20240959717699783No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG9260.1987606436753977No Hit
GGTGTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT8960.1923213139666915No Hit
AATATACATCGTATCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG8800.18888700478871487No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTGGATGTTGGATCTTC8390.18008658752014975No Hit
CACTAATATGGGCCTAAAGTTCCGGCAACTATTGTGGTTTCACATTTCTT8320.17858407725478495No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT8200.17600834537130247No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT8180.1755790567240554No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG7960.17085688160433754No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA7920.16999830430984336No Hit
GGGAAACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTC7690.16506148486650196No Hit
GGGATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTC7610.16334433027751363No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT7590.16291504163026657No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT7480.16055395407040762No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG7370.1581928665105487No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC7280.15626106759793684No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA7220.1549732016561956No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG7160.15368533571445436No Hit
GTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTTGC7130.15304140274358374No Hit
GGGTAAAGGTTCAGGTATTGTTTACGCAGAAAGGCCTTGTAAGTTGGCGA7100.15239746977271312No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGCGGGGTAGGCTGCCT7070.1517535368018425No Hit
GGTAAATATTTGGTAACATTTGGATAAAACCTAGCAGGCATAATCAATTG7070.1517535368018425No Hit
GAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGA7030.15089495950734835No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC7030.15089495950734835No Hit
CATTTACACCAAGACATTATTGAAAAATGTGAACAATTTGTAGGCCCACT7020.1506803151837248No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG6820.14638742871125401No Hit
GGGTTACTCTTTGCATTTCATGGGCTATGTCATTGGAAGTTATGGGTCAT6810.14617278438763046No Hit
CTACTGTACCTATCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT6760.14509956276951277No Hit
CTCTGGGATCTTGCAGATTTTGGTGGAAGTTTGTGGAGTTCCACTGCATG6630.14230918656240676No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG6580.14123596494428908No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA6510.13973345467892428No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT6500.13951881035530075No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAAATACAGGCCTCT6480.13908952170805366No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACTACC6440.13823094441355951No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA6350.13629914550094765No Hit
CATGTATTCAGTCGAAGCAGGCTTTTACTTTCTCGCCAACTTACAAGGCC6340.1360845011773241No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGA6320.13565521253007704No Hit
GTTTAATACCCTTATCCAATGGTAAATATTTGGTAACATTTGGATAAAAC6310.1354405682064535No Hit
CCATATTCTTGGGAACAAAAGCTACAGCATGGGGCAGAATCTTTCCACCA6250.13415270226471226No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6240.1339380579410887No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA6200.13307948064659456No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT6200.13307948064659456No Hit
ATCCCAGAGTGAGAGGCCTGTATTTCCCTGCTGGTGGCTCCAGTTCAGGA6150.13200625902847687No Hit
GTATGCATGTATTCAGTCGAAGCAGGCTTTTACTTTCTCGCCAACTTACA6080.13050374876311208No Hit
ATATGGGAGAGACAGTAGTCGGAACAGGGTTTACTGTTCCTGAACTGGAG5990.12857194985050024No Hit
GGCTTTTACTTTCTCGCCAACTTACAAGGCCTTTCTGCGTAAACAATACC5970.12814266120325316No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCCCATATCGTCAATCTTCTCG5940.12749872823238254No Hit
ATACTGCACTCAGGCAAGCAATTCTGTGCTGGGGGGAACTAATGACTCTA5940.12749872823238254No Hit
GGATAAGGGTATTAAACCTTATTACCCAGAACATCTAGTTAATCATTACT5870.12599621796701774No Hit
GCTTTAATGCCCTTGTATGCATGTATTCAGTCGAAGCAGGCTTTTACTTT5820.12492299634890006No Hit
GCTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG5810.12470835202527651No Hit
CCCCAAAAGGCCTCCGTGCGGTGGGGTGAATCCCGGCCCGAATGCTCCAG5810.12470835202527651No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5790.12427906337802944No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA5790.12427906337802944No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA5780.1240644190544059No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA5780.1240644190544059No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGGT5740.12320584175991174No Hit
CAATAGGTCTGTTAACAGGAAGTTTTCGAAAACATTCTGAAATTTTTTGT5700.12234726446541758No Hit
GAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAATACTAAC5670.12170333149454696No Hit
GTCCCGTGCTGGTAGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC5660.12148868717092341No Hit
TCCTAATACAAACAACTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGT5640.12105939852367635No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG5630.1208447542000528No Hit
GTAGAAGAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT5590.11998617690555863No Hit
CTATAACTGTTTGTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACAAT5590.11998617690555863No Hit
GAATTGGTGGGCGTTCACGGTGGTCTCCATGCGACGTGCAGAGGTGAAGC5520.11848366664019387No Hit
ACTACACACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGT5520.11848366664019387No Hit
TCACCATACTGCACTCAGGCAAGCAATTCTGTGCTGGGGGGAACTAATGA5510.11826902231657033No Hit
AACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGC5480.11762508934569972No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT5470.11741044502207618No Hit
GAATTAGAGGACAAACGGGCAACATACCTTGATAGTCCAGAAGAACCAAC5460.11719580069845263No Hit
GAGTTATCCGTCCCGAGAATGTTTGCTCCAGACCTGCTGCGAGCAAAACA5460.11719580069845263No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG5440.11676651205120554No Hit
ATATTATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACC5400.11590793475671139No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA5390.11569329043308785No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA5380.1154786461094643No Hit
CAAATATACATCGTATCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCC5380.1154786461094643No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT5380.1154786461094643No Hit
GGAATTAGAGGACAAACGGGCAACATACCTTGATAGTCCAGAAGAACCAA5360.11504935746221724No Hit
CATACTGCGGAACTCCTAGCCGCTTGTTTTGCTCGCAGCAGGTCTGGAGC5300.113761491520476No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG5290.11354684719685246No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA5280.11333220287322891No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC5240.11247362557873476No Hit
ATGTGATCTTGTGGCAATGACCCATAACTTCCAATGACATAGCCCATGAA5220.11204433693148767No Hit
ATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATTCAGTCGAAGC5140.11032718234249936No Hit
AGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTGCGGTGG5140.11032718234249936No Hit
TATCAATACTTCCGGAGACTACTGTTATTAGACGACGAGGCAGGTCCCCT5110.10968324937162875No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATAA5080.10903931640075812No Hit
CTATAAGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTC5070.10882467207713457No Hit
GACATAGCCCATGAAATGCAAAGAGTAACCCCATCTTTTTGTTTTGTTAG5050.10839538342988751No Hit
CTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATTCATTTACACC5050.10839538342988751No Hit
TTCTTCTACTGTACCTATCTTTAACCCTCATTGGAAAACACCCTCTTTTC5030.10796609478264042No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC4980.10689287316452273No Hit
TTATTTACACACTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAA4920.10560500722278149No Hit
GGTTTAATACCCTTATCCAATGGTAAATATTTGGTAACATTTGGATAAAA4920.10560500722278149No Hit
GTGTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTG4910.10539036289915794No Hit
GTATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTG4910.10539036289915794No Hit
GTACCTATCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATTCA4910.10539036289915794No Hit
GGGAAATACAGGCCTCTCACTCTGGGATCTTGCAGATTTTGGTGGAAGTT4880.10474642992828734No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC4860.10431714128104025No Hit
CAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAAC4840.10388785263379319No Hit
TCTAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGAGC4840.10388785263379319No Hit
CGGTAGTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTC4790.10281463101567548No Hit
GTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCTTCTCGGGGTCG4780.10259998669205195No Hit
GAATCGGTGGGCGTTCACGGTGGTCTCCATGCGACGTGCAGAGGTGAAGC4780.10259998669205195No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA4770.1023853423684284No Hit
CAGTTATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCA4720.1013121207503107No Hit
GTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTGCGGT4720.1013121207503107No Hit
CCCCTATCTTATCAATACTTCCGGAGACTACTGTTATTAGACGACGAGGC4700.10088283210306362No Hit
ATCTTCTAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCT4690.10066818777944007No Hit
CCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAAGAGTCCTCTTATGT4690.10066818777944007No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)