FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465887
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA24770.5316739896155076No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC20140.43229366777780875No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG18580.39880915329253663No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAATACTTCCGGA17280.3709053912214765No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAACAAC15250.32733259352589794No Hit
ATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT14280.30651209413441455No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT13340.28633552771380183No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC13280.28504766177206065No Hit
GTTATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC12120.26014892023173003No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA11650.25006063702142367No Hit
AATATACATCGTATCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG11100.23825519922212895No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA10840.23267444680791696No Hit
CTACACACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC10530.2260204727755872No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAAACTTCCACCAAAATCTGCAAG10490.22516189548109303No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTGCATTTCAT10420.22365938521572828No Hit
TTATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATA10350.22215687495036351No Hit
CTCTATAACTGTTTGTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA10320.2215129419794929No Hit
ATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACCTG10070.21614683388890438No Hit
CCCTTATCCAATGGTAAATATTTGGTAACATTTGGATAAAACCTAGCAGG9970.214000390652669No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC9770.20970750418019818No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTT9490.2036974631187391No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT9430.20240959717699783No Hit
GAATCTTTCCACCAGCAATCCTCTGGGATTCTTTCCCGACCACCAGTTGG9220.19790206638090352No Hit
ATGTTTCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACCTG9150.19639955611553875No Hit
GGTGTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT8920.19146273667219735No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA8350.17922801022565557No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG8320.17858407725478495No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT8310.17836943293116142No Hit
GCCTAGCAGCCATGGATACGATGTATATTTGCGGGAGAGAACAACAGAGT8020.17214474754607878No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC7890.16935437133897274No Hit
CACTAATATGGGCCTAAAGTTCCGGCAACTATTGTGGTTTCACATTTCTT7800.1674225724263609No Hit
GTTTAATACCCTTATCCAATGGTAAATATTTGGTAACATTTGGATAAAAC7490.16076859839403118No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT7460.16012466542316056No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC7410.15905144380504285No Hit
GCTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG7290.15647571192156037No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA7270.1560464232743133No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG7220.1549732016561956No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGA7210.15475855733257207No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTGGATGTTGGATCTTC7180.15411462436170142No Hit
CAAATATACATCGTATCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCC7100.15239746977271312No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT7040.15110960383097188No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT6930.14874851627111296No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6920.1485338719474894No Hit
GTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTTGC6880.14767529465299525No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGCGGGGTAGGCTGCCT6780.14552885141675986No Hit
GGGTTACTCTTTGCATTTCATGGGCTATGTCATTGGAAGTTATGGGTCAT6780.14552885141675986No Hit
GGTAAATATTTGGTAACATTTGGATAAAACCTAGCAGGCATAATCAATTG6750.14488491844588924No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG6740.1446702741222657No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT6710.14402634115139507No Hit
GGGTAAAGGTTCAGGTATTGTTTACGCAGAAAGGCCTTGTAAGTTGGCGA6640.14252383088603032No Hit
GAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGA6610.14187989791515968No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6500.13951881035530075No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGGT6420.13780165576631243No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA6370.13672843414819474No Hit
CATGTATTCAGTCGAAGCAGGCTTTTACTTTCTCGCCAACTTACAAGGCC6330.13586985685370057No Hit
GCTTTAATGCCCTTGTATGCATGTATTCAGTCGAAGCAGGCTTTTACTTT6310.1354405682064535No Hit
ATCCCAGAGTGAGAGGCCTGTATTTCCCTGCTGGTGGCTCCAGTTCAGGA6290.13501127955920642No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA6260.1343673465883358No Hit
GGGATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTC6220.13350876929384164No Hit
GTATGCATGTATTCAGTCGAAGCAGGCTTTTACTTTCTCGCCAACTTACA6180.13265019199934747No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA6150.13200625902847687No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCCCATATCGTCAATCTTCTCG6120.13136232605760623No Hit
GTCCCGTGCTGGTAGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC6100.13093303741035917No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG6090.13071839308673563No Hit
GGGAAACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTC6070.13028910443948855No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC6070.13028910443948855No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA6060.13007446011586501No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT6050.12985981579224148No Hit
GGCTTTTACTTTCTCGCCAACTTACAAGGCCTTTCTGCGTAAACAATACC5960.1279280168796296No Hit
CTCTGGGATCTTGCAGATTTTGGTGGAAGTTTGTGGAGTTCCACTGCATG5960.1279280168796296No Hit
GTATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTG5800.12449370770165297No Hit
GGATAAGGGTATTAAACCTTATTACCCAGAACATCTAGTTAATCATTACT5770.12384977473078236No Hit
CTACTGTACCTATCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT5740.12320584175991174No Hit
CATTTACACCAAGACATTATTGAAAAATGTGAACAATTTGTAGGCCCACT5730.1229911974362882No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC5710.12256190878904112No Hit
GGTTTAATACCCTTATCCAATGGTAAATATTTGGTAACATTTGGATAAAA5680.1219179758181705No Hit
CCATATTCTTGGGAACAAAAGCTACAGCATGGGGCAGAATCTTTCCACCA5600.12020082122918217No Hit
CCCCAAAAGGCCTCCGTGCGGTGGGGTGAATCCCGGCCCGAATGCTCCAG5590.11998617690555863No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAAATACAGGCCTCT5590.11998617690555863No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACTACC5510.11826902231657033No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT5510.11826902231657033No Hit
GTGTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTG5460.11719580069845263No Hit
ATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATTCAGTCGAAGC5380.1154786461094643No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA5350.1148347131385937No Hit
GTATTAAACCTTATTACCCAGAACATCTAGTTAATCATTACTTCCAAACC5280.11333220287322891No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT5280.11333220287322891No Hit
CTATAACTGTTTGTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACAAT5270.11311755854960538No Hit
ATACTGCACTCAGGCAAGCAATTCTGTGCTGGGGGGAACTAATGACTCTA5260.11290291422598184No Hit
ATATGGGAGAGACAGTAGTCGGAACAGGGTTTACTGTTCCTGAACTGGAG5240.11247362557873476No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC5200.1116150482842406No Hit
GAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAATACTAAC5190.11140040396061705No Hit
TTCTTCTACTGTACCTATCTTTAACCCTCATTGGAAAACACCCTCTTTTC5180.11118575963699351No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC5160.11075647098974645No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA5150.1105418266661229No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG5120.10989789369525227No Hit
ATGTGATCTTGTGGCAATGACCCATAACTTCCAATGACATAGCCCATGAA5060.10861002775351103No Hit
GTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCTTCTCGGGGTCG5030.10796609478264042No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG5030.10796609478264042No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA5000.10732216181176982No Hit
CCCCTATCTTATCAATACTTCCGGAGACTACTGTTATTAGACGACGAGGC5000.10732216181176982No Hit
CTCCCATATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAACATG4990.10710751748814627No Hit
AACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGC4950.10624894019365212No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4930.10581965154640503No Hit
ACCCTTCTCGGGGTCGCTTGGGACTCTCTCGTCCCCTTCTCCGTCTGCCG4910.10539036289915794No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4890.10496107425191088No Hit
TATACATCGTATCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCG4870.1045317856046638No Hit
AGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTGCGGTGG4870.1045317856046638No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA4830.10367320831016964No Hit
GTACCTATCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATTCA4820.1034585639865461No Hit
GTAGAAGAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT4790.10281463101567548No Hit
CAATAGGTCTGTTAACAGGAAGTTTTCGAAAACATTCTGAAATTTTTTGT4780.10259998669205195No Hit
ATCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC4770.1023853423684284No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC4740.10174140939755778No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC4720.1013121207503107No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG4700.10088283210306362No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG4670.100238899132193No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)