FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490501

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490501
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences390404
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA24900.6378008422044856No Hit
GTACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG18640.47745412444544627No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC16460.4216145326379853No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCACCCGTG15610.3998422147314064No Hit
CTATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATA14320.3667995204967162No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAGCTGAAAGCCAAA13770.35271155008657695No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC13650.3496378110880011No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC13290.34041659409227365No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA13140.3365744203440539No Hit
ATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATATT12000.30737389985758345No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT11100.2843208573682647No Hit
CTTATATAGAATACCAGCCTTCCAAAGAGTATTTAAATAATGTCTGGTTT10750.27535578528908516No Hit
GTATAAAGGGACTCACGATGCTGTACAGACTTGGCCCCCAATACCACATC9880.25307117754941033No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT8900.22796897572770772No Hit
ATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCCATGAA8500.21772317906578825No Hit
TCCTCAGGCCATGCAGTGGAATTCCACTGCCTTCCACCAAGCTCTGCAGG8270.2118318459851846No Hit
AGTCTGTACAGCATCGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT8170.20927039681970472No Hit
TATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCT8000.20491593323838897No Hit
CCTATTAATGATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT7900.2023544840729091No Hit
CTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC7870.20158604932326513No Hit
GCTCATCGGAACTGACAATTCTGTCGTCCTCTCGCGGAAATATACATCGT7760.19876845524123726No Hit
ATGTAAGACCTTGGGCAGGATCTGAAGGGCGTTCACGGTGGTCTCCATGC7720.19774387557504533No Hit
CCCATAGGTATTTTGCGAAAGCCCAGGACGATGGGATGGGAATACAGGTG7230.185192774664194No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT7140.18288747041526215No Hit
GTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG7070.18109445599942625No Hit
GTATTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGCTCCGA7050.18058216616633027No Hit
GGTTTTGTACAGCAACATGAGGGAAACATAGAGTTGCCTTGAGCAGGAGT7040.18032602124978228No Hit
CTCTTATATAGAATACCAGCCTTCCAAAGAGTATTTAAATAATGTCTGGT7040.18032602124978228No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG6870.1759715576684665No Hit
CCATTTACACAATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTAT6790.1739223983360826No Hit
GTCTAAGGGCAAATATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAA6740.1726416737533427No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC6690.17136094917060274No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA6640.17008022458786284No Hit
GGATCATATTGTAAAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA6560.16803106525547895No Hit
GGGTAAAGGTTCATGTACTGTTTACTGAGAAAGGCCTTGTAAGTTGGCGA6440.1649573262569031No Hit
GCCTTCCACCAAGCTCTGCAGGATCCCAGAGTCAGGGGTCTGTATTTTCC6390.16367660167416317No Hit
GTGTTTGATCTTTTTTACAATATGATCCTGTGGCAATGTTCCCCAACTTC6390.16367660167416317No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTCAG6360.1629081669245192No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGAAAATACAGACCCCT6340.16239587709142325No Hit
CAGTAAACCCTGCTCCGAATATTGCCTCTCACATCTCGTCAATCTCCGCG6320.16188358725832727No Hit
CACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTATTATCCAGATC6230.1595782830093954No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT6220.1593221380928474No Hit
CTTTCGGAGTGTGGATTCGCACTCCTCCAGCCTATAGACCACCAAATGCC6190.15855370334320343No Hit
GCCTAATCATCTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGC6150.15752912367701152No Hit
GCATGGCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGC5990.15343080501224374No Hit
GTACTGTAGAGGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAA5980.15317466009569575No Hit
GTATTTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGG5970.15291851517914776No Hit
CTCCTAGACACCGCCTCAGCTCTGTATCGAGAAGCCTTAGAGTCTCCTGA5950.15240622534605178No Hit
CTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTT5920.15163779059640783No Hit
GTACAAAACCTACGGATGGAAATTGCACCTGTATTCCCATCCCATCGTCC5860.1501009210971199No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTGTGGGT5790.148307906681284No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG5750.14728332701509206No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5720.1465148922654481No Hit
CTATTAATGATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTTG5620.14395344309996824No Hit
ATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG5570.14267271851722832No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA5560.14241657360068033No Hit
GTATATTTCCGCGAGAGGACGACAGAATTGTCAGTTCCGATGAGCTTTGC5550.14216042868413234No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTGTTTGATCTTTTTT5540.14190428376758435No Hit
GGCTGGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATATTCAAGTA5460.13985512443520046No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAA5420.13883054476900852No Hit
GTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAACAG5370.13754982018626857No Hit
GGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATATTCCTCTTCATCCT5360.13729367526972058No Hit
ACTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGATGGAAATTGC5340.13678138543662463No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG5320.13626909560352865No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCATGTACTGTTTACTG5310.13601295068698066No Hit
ATTGACCACAGTGTCAACAATTCCTCCTCCTGCCTCCACCAATCGGCAGT5250.13447608118769275No Hit
GTGGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAATACCGCCGTG5240.13421993627114476No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC5170.13242692185530888No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT5120.13114619727256893No Hit
TATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCT5100.13063390743947295No Hit
TTATTATCCAGATCAGGTAGTTAATCATTACTTCCAAACCAGACATTATT5100.13063390743947295No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGCCT5030.12884089302363705No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG5020.12858474810708906No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG5000.1280724582739931No Hit
GTATTAACATAATTGACTACTAGATCCCTGGATGCTGGATCTTCCAAATT4990.12781631335744512No Hit
ATTTAAATACTCTTTGGAAGGCTGGTATTCTATATAAGAGGGAAACCACA4990.12781631335744512No Hit
TCATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAG4990.12781631335744512No Hit
TTCTAAAACAGTGTTTGATCTTTTTTACAATATGATCCTGTGGCAATGTT4960.12704787860780115No Hit
ATACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA4940.12653558877470517No Hit
GGATTAAAGATAGGTACTGTAGAGGAATAAAGCCCAGTAAAGTTTCCCAC4910.12576715402506122No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTCCGCGAGAGGACGACAGAAT4890.12525486419196524No Hit
GTCCTGGCCCGAATGCTCCCACTCCTACCTGGTTGGCTGCTGGCCAGTGG4830.12371799469267733No Hit
CATATATCTTGCCTTACTTTTGGAAGAGAGACTGTACTTGAATATTTGGT4830.12371799469267733No Hit
GGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTAC4820.12346184977612934No Hit
GGTCCTAGGAATCCTGATGTGATGTTCTCCATGTTCGTCACAGGGTCCCC4800.12294955994303336No Hit
CATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGA4780.1224372701099374No Hit
GTTTTAGAAAACTTCCTGTTAACAGGCCTATTGATTGGAAAGTATGTCAA4750.12166883536029344No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTGCACTCAGGCA4750.12166883536029344No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA4750.12166883536029344No Hit
ACATAGAGTTGCCTTGAGCAGGAGTCGTGCAGGTTTTGCATGGTCCCGTA4740.12141269044374545No Hit
ATGTATATTTCCGCGAGAGGACGACAGAATTGTCAGTTCCGATGAGCTTT4740.12141269044374545No Hit
GTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCCTCTCGGGGCCG4580.11731437177897767No Hit
CTCCGAAAGAGACCAAATATTCAAGTACAGTCTCTCTTCCAAAAGTAAGG4580.11731437177897767No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC4520.11577750227968976No Hit
GGAAAATACAGACCCCTGACTCTGGGATCCTGCAGAGCTTGGTGGAAGGC4500.11526521244659378No Hit
ATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAAC4490.1150090675300458No Hit
GTAGAGGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT4480.11475292261349782No Hit
CTTTAAACCCATGTTAGTATTAACATAATTGACTACTAGATCCCTGGATG4480.11475292261349782No Hit
GTTTAAATGTATACCCAGAGACAAAAGAAAATTGGTAACAGCGGTATAAA4460.11424063278040184No Hit
GTCCAACTGATGATCGGGAAAGAATCCCAGAGGGTTGGGAACAGAAAGAT4450.11398448786385386No Hit
GGATATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAAT4450.11398448786385386No Hit
CTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAGATTCATTTACAAG4440.11372834294730587No Hit
GTACTTGAATATTTGGTCTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC4420.11321605311420989No Hit
GTCCTGGGCTTTCGCAAAATACCTATGGGAGTGGGCCTCAGTCCGTTTCT4420.11321605311420989No Hit
CCCTTAGACAAAGGAATTAAACCTTATTATCCAGATCAGGTAGTTAATCA4400.11270376328111392No Hit
GGTTTAAAGATCAGGCAACTATTGTGGTTTCATATATCTTGCCTTACTTT4390.11244761836456593No Hit
GTTCATGTACTGTTTACTGAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA4330.11091074886527802No Hit
ATTCTATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAAC4320.11065460394873003No Hit
CTCCCATAGGTATTTTGCGAAAGCCCAGGACGATGGGATGGGAATACAGG4300.11014231411563405No Hit
GTCTTACATAAGAGGACTCTTGGACTCCCAGCAATGTCAACGACCGACCT4280.10963002428253808No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCGTGAGTCCCTTTATACC4280.10963002428253808No Hit
GATAGGTACTGTAGAGGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAG4270.10937387936599009No Hit
GTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAGATTCA4260.1091177344494421No Hit
CAGTTGGACCCTGCATTCGGAGCCAACTCAAACAATCCAGATTGGGACTT4230.10834929969979815No Hit
GTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG4220.10809315478325016No Hit
ATTTTGGGGTGGAGCCCTCAGGCTCAGGGCATATTGACCACAGTGTCAAC4220.10809315478325016No Hit
CCCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC4210.10783700986670218No Hit
GGCCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGGGCTTT4200.10758086495015419No Hit
TAAATACACAGTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCT4190.1073247200336062No Hit
TTCAAGTACAGTCTCTCTTCCAAAAGTAAGGCAAGATATATGAAACCACA4190.1073247200336062No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCCTCTACAG4170.10681243020051025No Hit
CTATTGTGGTTTCATATATCTTGCCTTACTTTTGGAAGAGAGACTGTACT4170.10681243020051025No Hit
CCTGTATTCCCATCCCATCGTCCTGGGCTTTCGCAAAATACCTATGGGAG4150.10630014036741428No Hit
GTTTTGTACAGCAACATGAGGGAAACATAGAGTTGCCTTGAGCAGGAGTC4150.10630014036741428No Hit
GAAATATACATCGTTTCCATGGCTGCTAGGCTGTACTGCCAACTGGATCC4130.1057878505343183No Hit
CCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTTGGTCATCAAAAC4130.1057878505343183No Hit
ATCATATTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTATTGGTTCT4130.1057878505343183No Hit
GAATACAGGTGCAATTTCCATCCGTAGGTTTTGTACAGCAACATGAGGGA4100.10501941578467434No Hit
GCATGGGAGGTTGGTCATCAAAACCTCGCAAAGGCATGGGGACGAATCTT4100.10501941578467434No Hit
GTACAGCATCGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC4020.10297025645229045No Hit
TGTATACCCAGAGACAAAAGAAAATTGGTAACAGCGGTATAAAGGGACTC3950.10117724203645455No Hit
CTCTTAGAGGTGGAGAGATGGGAGTAGGCTGCCTTCCTGACTGCCGATTG3910.1001526623702626No Hit
GTATCGAGAAGCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTG3910.1001526623702626No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)