FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490502

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490502
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences574508
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTG31160.5423771296483252No Hit
CCTAGTACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTC26550.4621345568730113No Hit
ATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTCACGGTGGTCTCCATGC26230.4565645735133367No Hit
GTCCAAGAGTCCTCTTATGTAAGAGAGTCCAAGAGTCCTCTTATGTAAGA21580.37562575281806343No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTC19290.3357655594003913No Hit
GTGTCAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC19150.3333286916805336No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTGGATGCTGGATCTTC18740.3261921505009504No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA18730.32601808852096054No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAAAAAGTGAAAGCCTGCTT18700.32549590258099104No Hit
CTCTATGGAAGGCGGGTGTTTTATATAAGAGAGAATCAACACATAGCGCC17950.31244125408175344No Hit
TCCATAGAGTGTGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTAGA17430.303390031122282No Hit
CACTCATATGGGCCTAAAGTTCAGACAACTATTGTGGTTTCACATTTCTT16800.2924241263829224No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA16130.2807619737236035No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC15760.27432168046397964No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGATATTCTCTAAATTTCAT15520.27014419294422354No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAAATCTCCTCCC14850.2584820402849046No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC14620.25447861474513844No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG14200.2471680115855654No Hit
ATATGAGTGTTGACATAACTGACTACTAGGTCCCTGGATGCTGGATCTTC13250.2306321234865311No Hit
CTATTTACACACTCTATGGAAGGCGGGTGTTTTATATAAGAGAGAATCAA13020.22662869794676488No Hit
CTATGTATCCCTCCTGTTGCTGTACAAAACCTTCGGATGGAAACTGCACC12760.22210308646702917No Hit
CTCTAGGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA12750.22192902448703936No Hit
ACACAAGTCTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTGGTCTATA12660.22036246666713083No Hit
ACAGTAGTCTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTGGTCTATA12650.220188404687141No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT12560.21862184686723246No Hit
ATGTAAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGATTTG12320.21444435934747644No Hit
CACTAATATGGGCCTAAAGTTCAGACAACTATTGTGGTTTCACATTTCTT11980.20852625202782207No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG11920.20748188014788307No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA11840.20608938430796436No Hit
ATATAAGAGAGAATCAACACATAGCGCCTCATTTTGTGGGTCACCATATT11710.20382657856809652No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC11480.19982315302833034No Hit
GTCCTAGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTCCAGC11280.19634191342853363No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT11090.19303473580872677No Hit
GTGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT10820.18833506234900121No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC10140.17649884770969246No Hit
CTTTAGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGGA9870.17179917424996693No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC9700.16884012059013975No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG9590.16692543881025157No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTCCG9220.16048514555062765No Hit
CTAGTAAACTGAGCCAAGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT9120.1587445257507293No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA9100.15839640179074965No Hit
GTATCGGGATGCCTTAGAATCTCCTGAGCATTGTTCACCTCACCATACTG9040.15735202991081065No Hit
CAATAGGCCTGTTAACAGGGAGTTTTCTAAAACATTCTTTGATTTTAAGT8690.15125986061116642No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG8570.14917111685128842No Hit
CTCTTGGACTCTCTGCAATGTCAACGACCGACCTTGAGGCATACTTCAAA8420.14656018715144087No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC8400.1462120631914612No Hit
ATGTATCTGCGGCGTTTTATCATCTTCCTCTTCATCCTGCTGCTATGCCT8290.14429738141157303No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG8230.14325300953163403No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCAGGGTCCCGTG8130.1415123897317357No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACAAAACCTTCGGATGGAAACTGC8110.141164265771756No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTGTCAGTCCCGAGAATGTTT8100.14099020379176616No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT8100.14099020379176616No Hit
GACTACTGTTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCT8020.1395977079518475No Hit
GAGTCCAAGAGTCCTCTTATGTAAGAGAGTCCAAGAGTCCTCTTATGTAA8000.13924958399186782No Hit
GTTTGTAGGCCCGCTCACAGTCAATGAGAAAAGAAGGTTGAAATTGATTA7940.13820521211192882No Hit
CCCCTACTCTGCCTGCCATTCCGACCGACCACGGGGCGCACCTCTCTTTA7830.13629053033204064No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT7670.13350553865220327No Hit
TTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAAAAAGTGAAAGCCTGCT7660.13333147667221346No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTTACATAAGAGGACTCTTGGA7460.12985023707241675No Hit
GCTTTGGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGG7420.1291539891524574No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA7420.1291539891524574No Hit
GGATAACCACATTGAGTGAAAGGGGCAGCAAAACCCAGAAGACCCGCAAT7280.12671712143259972No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC7270.12654305945260988No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA7260.12636899747262004No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG7160.1246283776727217No Hit
GCTTTAATGCCCTTGTATGCATGTATTCAATCTAAGCAGGCTTTCACTTT7150.12445431569273187No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGAATGG7140.12428025371274204No Hit
GACTTGTGTTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCT7110.12375806777277254No Hit
CTCTTATATAAAACACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTGGT7100.1235840057927827No Hit
GTACAAAACCTTCGGATGGAAACTGCACCTGTATTCCCATCCCATCATCC7080.12323588183280301No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTTTCGCCAACTTACA6990.12166932401289451No Hit
CTTCCCGGGGCCGCTTGGGCCCCTGTCGTCCCCTACTCTGCCTGCCATTC6930.1206249521329555No Hit
CAGACACATCCAGCGATAACCAGGACAAGTTGGAGGACAGGAGGTTGGTG6930.1206249521329555No Hit
GCACGGGACCCTGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG6880.11975464223300633No Hit
CTCCAAAAGACACCAAATACTCTAGGACCGTTTCTCTTCCAAAAGTGAGA6830.11888433233305715No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGAGTGAAA6790.11818808441309782No Hit
CATGCATACAAGGGCATTAAAGCAGGATAACCACATTGAGTGAAAGGGGC6770.11783996045311815No Hit
GGCCTATTGATTGGAAAGTATGTCAACGAATTGCGGGTCTTCTGGGTTTT6640.11557715471325031No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6490.11296622501340277No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA6400.11139966719349426No Hit
ATTATGCCCTGTGCCTGAGGGCTCCACCCCAAAAGACCTCCGTGCGGCGG6380.11105154323351459No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA6300.10965904739359592No Hit
GTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAAAC6220.10826655155367723No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA6120.10652593175377889No Hit
GAATACAGGTGCAGTTTCCATCCGAAGGTTTTGTACAGCAACAGGAGGGA6090.10600374581380939No Hit
GTATATTTGCGGGAGAGAACAACAGAGTTGTCAGTCCCGAGAATGTTTGC6000.10443718799390087No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT5980.10408906403392121No Hit
GAATAAAGCCCCGTAAAATTCCCCACCTTATGAGTCCAAGGAATACTAAC5840.10165219631406353No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5840.10165219631406353No Hit
TTGTAGGCCCGCTCACAGTCAATGAGAAAAGAAGGTTGAAATTGATTATG5780.1006078244341245No Hit
AGCTGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCTAGGA5770.10043376245413467No Hit
GTCATGGGCCATCAGCGCATGCGTGGAACCTTTCTGGCTCCTCTGCCGAT5760.10025970047414484No Hit
GAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGATTTGGTGGGCGT5750.100085638494155No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)