FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490503

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490503
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences574508
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTCACGGTGGTCTCCATGC20450.35595674907921215No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA20060.3491683318596086No Hit
CCTAGTACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTC19460.3387246130602185No Hit
GTGTCAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC19000.3307177619806861No Hit
GTCCAAGAGTCCTCTTATGTAAGAGAGTCCAAGAGTCCTCTTATGTAAGA18700.32549590258099104No Hit
CCTAAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTG18130.31557436972157044No Hit
TCCATAGAGTGTGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTAGA17130.29816817172258697No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTGGATGCTGGATCTTC15990.2783251060037458No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAAAAAGTGAAAGCCTGCTT15850.2758882382838881No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTC15840.2757141763038983No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC15710.27345137056403046No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG15590.2713626268041524No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGATATTCTCTAAATTTCAT15270.2657926434444777No Hit
CTCTATGGAAGGCGGGTGTTTTATATAAGAGAGAATCAACACATAGCGCC15260.26561858146448786No Hit
CACTCATATGGGCCTAAAGTTCAGACAACTATTGTGGTTTCACATTTCTT15030.2616151559247217No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA14270.2483864454454942No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAAATCTCCTCCC13810.24037959436596182No Hit
CTCTAGGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA13080.2276730698267039No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12780.22245121042700883No Hit
CTATGTATCCCTCCTGTTGCTGTACAAAACCTTCGGATGGAAACTGCACC11720.20400064054808636No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA11670.20313033064813718No Hit
CACTAATATGGGCCTAAAGTTCAGACAACTATTGTGGTTTCACATTTCTT11570.20138971084823887No Hit
CTATTTACACACTCTATGGAAGGCGGGTGTTTTATATAAGAGAGAATCAA11520.20051940094828968No Hit
GTCCTAGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTCCAGC11440.19912690510837097No Hit
CTTTAGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGGA11320.19703816134849297No Hit
ATATGAGTGTTGACATAACTGACTACTAGGTCCCTGGATGCTGGATCTTC11110.19338285976870645No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC11080.19286067382873695No Hit
GTGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT11050.19233848788876745No Hit
ACAGTAGTCTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTGGTCTATA10820.18833506234900121No Hit
ACACAAGTCTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTGGTCTATA10330.17980602532949932No Hit
CCCCTACTCTGCCTGCCATTCCGACCGACCACGGGGCGCACCTCTCTTTA10260.17858759146957048No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT9960.17336573206987543No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTCCG9770.17005855445006857No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT9600.16709950079024138No Hit
ATGTAAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGATTTG9400.1636182611904447No Hit
CTCTTGGACTCTCTGCAATGTCAACGACCGACCTTGAGGCATACTTCAAA9320.16222576535052602No Hit
ATATAAGAGAGAATCAACACATAGCGCCTCATTTTGTGGGTCACCATATT9250.16100733149059718No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG9120.1587445257507293No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG9030.1571779679308208No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC8940.15561141011091228No Hit
GTATCGGGATGCCTTAGAATCTCCTGAGCATTGTTCACCTCACCATACTG8910.15508922417094279No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC8720.15178204655113592No Hit
GCTTTGGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGG8670.15091173665118676No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC8610.14986736477124774No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG8570.14917111685128842No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACAAAACCTTCGGATGGAAACTGC8460.14725643507140024No Hit
CAATAGGCCTGTTAACAGGGAGTTTTCTAAAACATTCTTTGATTTTAAGT8280.14412331943158319No Hit
CTTCCCGGGGCCGCTTGGGCCCCTGTCGTCCCCTACTCTGCCTGCCATTC8270.14394925745159337No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTTACATAAGAGGACTCTTGGA8130.1415123897317357No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA8120.14133832775174585No Hit
ATGTATCTGCGGCGTTTTATCATCTTCCTCTTCATCCTGCTGCTATGCCT8090.14081614181177632No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTGTCAGTCCCGAGAATGTTT8080.1406420798317865No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA7990.139075522011878No Hit
CTAGTAAACTGAGCCAAGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT7970.13872739805189832No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT7970.13872739805189832No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG7860.13681271627201014No Hit
GGCCTATTGATTGGAAAGTATGTCAACGAATTGCGGGTCTTCTGGGTTTT7680.1336796006321931No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT7540.13124273291233543No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG7340.12776149331253872No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG7300.12706524539257938No Hit
GTTTGTAGGCCCGCTCACAGTCAATGAGAAAAGAAGGTTGAAATTGATTA7110.12375806777277254No Hit
GGATAACCACATTGAGTGAAAGGGGCAGCAAAACCCAGAAGACCCGCAAT7020.12219150995286401No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC6940.12079901411294533No Hit
CTCCAAAAGACACCAAATACTCTAGGACCGTTTCTCTTCCAAAAGTGAGA6930.1206249521329555No Hit
CTCTTATATAAAACACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTGGT6920.12045089015296567No Hit
GCTTTAATGCCCTTGTATGCATGTATTCAATCTAAGCAGGCTTTCACTTT6880.11975464223300633No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCAGGGTCCCGTG6880.11975464223300633No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC6830.11888433233305715No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTTTCGCCAACTTACA6730.1171437125331588No Hit
GTACAAAACCTTCGGATGGAAACTGCACCTGTATTCCCATCCCATCATCC6720.11696965055316896No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA6710.11679558857317913No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA6660.11592527867322996No Hit
CTCTTGGACTCTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAA6630.11540309273326047No Hit
TTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAAAAAGTGAAAGCCTGCT6480.11279216303341294No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGAGTGAAA6460.11244403907343327No Hit
GTTTAATACCCTTGTCCAAGGGCAAATATTTGGTAACATTAGGGTAAAAC6370.11087748125352476No Hit
GCACGGGACCCTGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG6240.1086146755136569No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT6180.1075703036337179No Hit
GACTACTGTTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCT6170.10739624165372805No Hit
GCCTGAGGATGAGTGTTTCTCAGAGGTGGAGACAGCGGGGTAGGCTGCCG6150.10704811769374839No Hit
GCTTTAGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGG6150.10704811769374839No Hit
CTGCCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAGACC6110.10635186977378905No Hit
CCTCTGCACGTCGCATGGAGACCACCGTGAACGCCCACCAAATCTTGCCC6090.10600374581380939No Hit
CTTTGGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGGA6070.10565562185382972No Hit
CATGCATACAAGGGCATTAAAGCAGGATAACCACATTGAGTGAAAGGGGC6060.10548155987383986No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6050.10530749789385004No Hit
GTATTATGCCCTGTGCCTGAGGGCTCCACCCCAAAAGACCTCCGTGCGGC6040.10513343591386021No Hit
ATTATGCCCTGTGCCTGAGGGCTCCACCCCAAAAGACCTCCGTGCGGCGG5990.10426312601391104No Hit
GAATAAAGCCCCGTAAAATTCCCCACCTTATGAGTCCAAGGAATACTAAC5980.10408906403392121No Hit
GAGTCCAAGAGTCCTCTTATGTAAGAGAGTCCAAGAGTCCTCTTATGTAA5940.10339281611396185No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG5830.10147813433407367No Hit
GACTTGTGTTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCT5790.10078188641411434No Hit
AGCTGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCTAGGA5760.10025970047414484No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA5760.10025970047414484No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)