FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490504

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490504
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences373672
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC31190.8346892461838189No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG17690.4734098353636344No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA17390.4653814040120748No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC16390.43861996617354254No Hit
CTATTTACACACGCTATGGAAGGCGGGTGTTTTATATAAGAGAGAATCAA11800.31578496649467985No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG11170.29892526065640457No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCCACCAATCGCCAGTC10940.2927701299535422No Hit
GTGTAAAAGGGGCAGCAAACCCCAGAAGACCCACAATTCTTTGACACACT10550.2823331691965146No Hit
GTACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA10330.2764456528720375No Hit
GCCTAGTCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC10090.2700229077907898No Hit
TCCATAGCGTGTGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTAGA9670.25878310389860626No Hit
ATGTGGTACTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC8810.23576826735746859No Hit
ATATAAGAGAGAATCAACACATAGCGCCTCATTTTGTGGGTCACCATATT8740.2338949667087713No Hit
ATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTCACGGTGGTCTCCATGC8700.23282450919523004No Hit
GTACAGACTTGGCCCCCAGTACCACATCATCCATATAACTGAAAGCCAAA8640.2312188229249181No Hit
GTGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT8540.2285426791410649No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC8520.22800745038429426No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA8520.22800745038429426No Hit
GGTTTGTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT8200.21944379027596397No Hit
CTATGTATCCCTCCTGTTGCTGTACAAAACCTTTGGATGGAAACTGCACC7970.21328865957310153No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCTCTAAATTTCAT7910.2116829733027896No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTC7800.20873921514055105No Hit
CTCTTGGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA7710.20633068573508317No Hit
CATTTACACCAAGATATTATCAAAAAATGTGAACAGTTTGTAGGCCCGCT7680.2055278425999272No Hit
CTCTTATATAAAACACCCGCCTTCCATAGCGTGTGTAAATAGTGTCTGGT7610.20365454195122998No Hit
CCGACTACTGCCTCTCACTTATCGTCAATCTTCTCGAGGATTGGGGACCC7590.2031193131944593No Hit
CTCTAAGGCATCCCGATACAGAGCTGTAGCGGTATCTAGCAGATCTCGGA7560.20231647005930334No Hit
CTAGTAAACTGAGCCAAGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT7530.2015136269241474No Hit
GTTTGTAGGCCCGCTCACAGTCAATGAGAAAAGAAGGTTGAAATTGATTA7510.20097839816737673No Hit
GGCTTTCACTTTTTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC7430.19883748314029415No Hit
CTCCAAAAGATACCAAATACTCTTGGACCGTTTCTCTTCCAAAAGTGAGA7410.19830225438352353No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACTTCACCATACGGCACTCAGGCA7410.19830225438352353No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAACCCCAGAAGACCCACAAT7330.19616133935644095No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCAGGGTCCCGTG7310.1956261105996703No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC7300.19535849622128498No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC7280.19482326746451434No Hit
AGCTGGAGGAGTGCGAATCCACACTCCAAAAGATACCAAATACTCTTGGA7250.19402042432935837No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG7250.19402042432935837No Hit
GTATCGGGATGCCTTAGAGTCTCCTGAGCATTGTTCACTTCACCATACGG7130.1908090517887345No Hit
TCTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTCCG7020.18786529362649595No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCAGCTTATAGACCACCAAATGCC6990.18706245049134No Hit
TTGTAGGCCCGCTCACAGTCAATGAGAAAAGAAGGTTGAAATTGATTATG6970.18652722173456937No Hit
CATTTTTTGATAATATCTTGGTGTAAATGTATATTAGGAAAAGAAGGTGT6930.18545676422102808No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACGGAGT6730.18010447665332163No Hit
CGCTATGGAAGGCGGGTGTTTTATATAAGAGAGAATCAACACATAGCGCC6570.1758226465991565No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT6540.1750198034640005No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACAAAACCTTTGGATGGAAACTGC6460.17287888843691795No Hit
GTCCAAGAGTATTTGGTATCTTTTGGAGTGTGGATTCGCACTCCTCCAGC6430.17207604530176196No Hit
GTTTAATACCCTTGTCCAAGGGCAAATATTTGGTAGACTTAGGGTAAAAC6350.1699351302746794No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT6000.16056862703119312No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC5990.16030101265280783No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAAATACAGGCCTCT5990.16030101265280783No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGCGGGGTAGGCTGCCG5930.15869532638249587No Hit
GTGTAAATGTATATTAGGAAAAGAAGGTGTTTTCCAATGAGGGTTAAAGA5930.15869532638249587No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT5930.15869532638249587No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA5880.15735725449056928No Hit
TCCCGATACAGAGCTGTAGCGGTATCTAGCAGATCTCGGACGGAAGGAAA5870.15708964011218396No Hit
GTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTGCGGC5800.1552163394634867No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA5780.15468111070671603No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG5550.14852598000385367No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5500.14718790811192703No Hit
CAATAGGCCTGTTAACAGGGAGTTTTCTAAAACATTCTTTGATTTTGAGT5480.1466526793551564No Hit
GCTTTAGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTTCTGTGG5480.1466526793551564No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5400.14451176432807383No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCAGGGTCCC5380.14397653557130316No Hit
ATATGATCTTGTGGCAATGATCCCCAACTTCCAATGACATAACCCATGAA5360.14344130681453254No Hit
TCCAGGGACCTAGTAGTCAGTTATGTCAACACTCATATGGGCCTAAAGTT5240.14022993427390867No Hit
GTGTTGATTCTCTCTTATATAAAACACCCGCCTTCCATAGCGTGTGTAAA5220.13969470551713803No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCTCACTTATCGTCAATCTTCTCG5150.13782140486844077No Hit
GTCCAAGGAATACTAACATTGAGATTGCCGAGATGGAGATCTTCTGCGAC5090.13621571859812884No Hit
GAATACAGGTGCAGTTTCCATCCAAAGGTTTTGTACAGCAACAGGAGGGA5050.13514526108458755No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA4990.1335395748142756No Hit
ATCCCAGAGTGAGAGGCCTGTATTTCCCTGCTGGTGGCTCCAGTTCAGGA4980.1332719604358903No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC4940.132201502922349No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC4890.13086343103042242No Hit
GGGACCTAGTAGTCAGTTATGTCAACACTCATATGGGCCTAAAGTTCAGG4880.13059581665203707No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTGCCGAGATGGAGATCT4850.12979297351688113No Hit
CTCTGGGATCTTGCAGAGTTTGGTGGAAGGTTGTGGAGTTCCACTGCATG4850.12979297351688113No Hit
GTACAGTAGAAGAATAAAGTCCCGTAAGGTTTCCCACCTTATGAGTCCAA4840.12952535913849578No Hit
GTACAAAACCTTTGGATGGAAACTGCACCTGTATTCCCATCCCATCATCC4800.1284549016249545No Hit
GCACGGGACCCTGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG4800.1284549016249545No Hit
CTACTGTACCTATCTTTAACCCTCATTGGAAAACACCTTCTTTTCCTAAT4780.12791967286818387No Hit
GAATACTAACATTGAGATTGCCGAGATGGAGATCTTCTGCGACGCGGCGA4700.1257787578411013No Hit
GGGTTAAAGATAGGTACAGTAGAAGAATAAAGTCCCGTAAGGTTTCCCAC4600.12310261405724807No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4570.12229977092209211No Hit
GATAGGTACAGTAGAAGAATAAAGTCCCGTAAGGTTTCCCACCTTATGAG4540.12149692778693615No Hit
CCCCAAAAGGCCTCCGTGCGGCGGGGTGAACCCTAGCCCGAATGCTCCAG4520.12096169903016549No Hit
ATGTATATTTGCGGGAGAGAACAACGGAGTTGTCAGTCCCGAGAATGTTT4500.12042647027339486No Hit
GGCCTACAAACTGTTCACATTTTTTGATAATATCTTGGTGTAAATGTATA4480.1198912415166242No Hit
ATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTGCGGCGG4430.1185531696246976No Hit
GATATGATCTTGTGGCAATGATCCCCAACTTCCAATGACATAACCCATGA4360.11667986897600034No Hit
ATATAAAACACCCGCCTTCCATAGCGTGTGTAAATAGTGTCTGGTTTGGA4340.11614464021922971No Hit
GTACCTATCTTTAACCCTCATTGGAAAACACCTTCTTTTCCTAATATACA4330.11587702584084436No Hit
GAACAAATGGCACTAGTAAACTGAGCCAAGAGAAACGGGCTGAGGCCCAC4260.11400372519214713No Hit
GGATAATAAGGTTTAATACCCTTGTCCAAGGGCAAATATTTGGTAGACTT4240.11346849643537647No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAATCTAGTCTCCTCC4230.11320088205699116No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTACTGGGGGC4230.11320088205699116No Hit
GAATAAAGTCCCGTAAGGTTTCCCACCTTATGAGTCCAAGGAATACTAAC4200.11239803892183518No Hit
ATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATTCAATCTAAGC4180.11186281016506455No Hit
CCCCTATCTTATCAACACTTCCGGAGACTACTGTTGTTAGACGACGAGGC4160.11132758140829391No Hit
GAATCTTTCCACCAGCAATCCTCTAGGATTCTTTCCCGACCACCAGTTGG4120.11025712389475262No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA4120.11025712389475262No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGGCATGGACATTGA4100.10972189513798199No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAACCCCAGAAGACCCACAATTCT4090.10945428075959665No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC4090.10945428075959665No Hit
CTATAAGCTGGAGGAGTGCGAATCCACACTCCAAAAGATACCAAATACTC4070.108919052002826No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACACTATT4060.1086514376244407No Hit
CCATATTCTTGGGAACAAGAGCTACAGCATGGGGCAGAATCTTTCCACCA4040.10811620886767004No Hit
CTAGCTACCTGGGTGGGTACTAATTTAGATGATCAAGCATCCAGGGACCT4040.10811620886767004No Hit
TCCTAGTACAAAGATCATTAATCTAGTCTCCTCCCCCAACTCCTCCCAGT4040.10811620886767004No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTTTCGCCAACTTACA4030.10784859448928473No Hit
TCCTTGGACTCATAAGGTGGGAAACCTTACGGGACTTTATTCTTCTACTG4010.10731336573251407No Hit
GTTTTATATAAGAGAGAATCAACACATAGCGCCTCATTTTGTGGGTCACC3960.10597529384058747No Hit
GTCCCAGGCGGCCCCGAGAAGGGTCGTCCGCAGGATTCAGCGCCGACGGG3950.10570767946220215No Hit
GGCCTATTGATTGGAAAGTGTGTCAAAGAATTGTGGGTCTTCTGGGGTTT3930.10517245070543149No Hit
CTCTTGGACTCTCTGCAATGTCAACGACCGACCTTGAGGCATACTTCAAA3880.1038343788135049No Hit
GAATAACCCCATCTCTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGA3860.10329915005673423No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG3860.10329915005673423No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA3860.10329915005673423No Hit
CTCTTTACGCGGCCTCCCCGTCTGTGCCTTTTCATCTGCCGGTCCGTGTG3790.101425849408037No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAAAAAGTGAAAGCCTGCTT3770.10089062065126635No Hit
GACATAACCCATGAAATTTAGAGAATAACCCCATCTCTTTGTTTTGTTAG3770.10089062065126635No Hit
CCTACAAACTGTTCACATTTTTTGATAATATCTTGGTGTAAATGTATATT3750.10035539189449572No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)