FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences373672
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC27560.7375452268299472No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA20120.5384401293112676No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG18700.500438887580552No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC15090.4038300969834507No Hit
GCCTAGTCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC11530.3085593782782762No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG10790.2887559142777623No Hit
CTATTTACACACGCTATGGAAGGCGGGTGTTTTATATAAGAGAGAATCAA10350.2769808816288082No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCCACCAATCGCCAGTC10310.27591042411526684No Hit
GTGTAAAAGGGGCAGCAAACCCCAGAAGACCCACAATTCTTTGACACACT10120.27082575092594574No Hit
GTACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA9770.2614592476824595No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC9700.25958594703376225No Hit
GTACAGACTTGGCCCCCAGTACCACATCATCCATATAACTGAAAGCCAAA9460.2531632019525145No Hit
ATGTGGTACTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC9120.24406431308741355No Hit
GGTTTGTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT8730.233627352330386No Hit
TCCATAGCGTGTGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTAGA8720.23335973795200068No Hit
GTGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT8530.22827506476267956No Hit
CTCTTGGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA8440.22586653535721168No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAACCCCAGAAGACCCACAAT8220.2199790190327346No Hit
ATATAAGAGAGAATCAACACATAGCGCCTCATTTTGTGGGTCACCATATT8130.2175704896272667No Hit
CTCCAAAAGATACCAAATACTCTTGGACCGTTTCTCTTCCAAAAGTGAGA7950.2127534308163309No Hit
CATTTACACCAAGATATTATCAAAAAATGTGAACAGTTTGTAGGCCCGCT7750.20740114324862444No Hit
CTCTTATATAAAACACCCGCCTTCCATAGCGTGTGTAAATAGTGTCTGGT7710.20633068573508317No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA7690.20579545697831256No Hit
CTATGTATCCCTCCTGTTGCTGTACAAAACCTTTGGATGGAAACTGCACC7680.2055278425999272No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCTCTAAATTTCAT7670.20526022822154188No Hit
TCTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTCCG7550.20204885568091804No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACTTCACCATACGGCACTCAGGCA7530.2015136269241474No Hit
GTTTGTAGGCCCGCTCACAGTCAATGAGAAAAGAAGGTTGAAATTGATTA7360.19696418249159692No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCAGCTTATAGACCACCAAATGCC7210.19294996681581708No Hit
GTATCGGGATGCCTTAGAGTCTCCTGAGCATTGTTCACTTCACCATACGG7180.1921471236806611No Hit
ATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTCACGGTGGTCTCCATGC7160.19161189492389047No Hit
AGCTGGAGGAGTGCGAATCCACACTCCAAAAGATACCAAATACTCTTGGA6970.18652722173456937No Hit
GTTTAATACCCTTGTCCAAGGGCAAATATTTGGTAGACTTAGGGTAAAAC6920.18518914984264276No Hit
GCTTTAGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTTCTGTGG6720.1798368622749363No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC6600.17662548973431244No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC6580.1760902609775418No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC6490.17368173157207392No Hit
TTGTAGGCCCGCTCACAGTCAATGAGAAAAGAAGGTTGAAATTGATTATG6480.17341411719368857No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTC6380.17073797340983538No Hit
CCGACTACTGCCTCTCACTTATCGTCAATCTTCTCGAGGATTGGGGACCC6370.17047035903145003No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6370.17047035903145003No Hit
GGCTTTCACTTTTTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC6330.16939990151790874No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT6300.1685970583827528No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCAGGGTCCCGTG6210.1661885289772849No Hit
CATTTTTTGATAATATCTTGGTGTAAATGTATATTAGGAAAAGAAGGTGT6160.16485045708535828No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT6110.16351238519343167No Hit
GTCCAAGAGTATTTGGTATCTTTTGGAGTGTGGATTCGCACTCCTCCAGC6100.16324477081504635No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT6050.16190669892311974No Hit
GTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTGCGGC5990.16030101265280783No Hit
GAATCTTTCCACCAGCAATCCTCTAGGATTCTTTCCCGACCACCAGTTGG5990.16030101265280783No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT5960.15949816951765183No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGCGGGGTAGGCTGCCG5950.15923055513926654No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5900.15789248324733993No Hit
GGGACCTAGTAGTCAGTTATGTCAACACTCATATGGGCCTAAAGTTCAGG5890.15762486886895458No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAAATACAGGCCTCT5850.1565544113554133No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACAAAACCTTTGGATGGAAACTGC5830.15601918259864267No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC5760.1541458819499454No Hit
GTGTAAATGTATATTAGGAAAAGAAGGTGTTTTCCAATGAGGGTTAAAGA5750.1538782675715601No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG5710.1528078100580188No Hit
CTAGTAAACTGAGCCAAGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5520.14772313686869767No Hit
CTCTAAGGCATCCCGATACAGAGCTGTAGCGGTATCTAGCAGATCTCGGA5480.1466526793551564No Hit
CTCTGGGATCTTGCAGAGTTTGGTGGAAGGTTGTGGAGTTCCACTGCATG5450.14584983622000042No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCTCACTTATCGTCAATCTTCTCG5380.14397653557130316No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCAGGGTCCC5360.14344130681453254No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA5360.14344130681453254No Hit
GTACAGTAGAAGAATAAAGTCCCGTAAGGTTTCCCACCTTATGAGTCCAA5350.1431736924361472No Hit
GAATACTAACATTGAGATTGCCGAGATGGAGATCTTCTGCGACGCGGCGA5340.14290607805776187No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC5320.14237084930099125No Hit
CGCTATGGAAGGCGGGTGTTTTATATAAGAGAGAATCAACACATAGCGCC5310.1421032349226059No Hit
GCAACAGGAGGGATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTC5270.1410327774090646No Hit
GTGTTGATTCTCTCTTATATAAAACACCCGCCTTCCATAGCGTGTGTAAA5220.13969470551713803No Hit
ATATGATCTTGTGGCAATGATCCCCAACTTCCAATGACATAACCCATGAA5170.13835663362521142No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACGGAGT5140.13755379049005545No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGGCATG5070.1356804898413582No Hit
GAACAAATGGCACTAGTAAACTGAGCCAAGAGAAACGGGCTGAGGCCCAC5010.13407480357104626No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC5000.13380718919266096No Hit
GAATACAGGTGCAGTTTCCATCCAAAGGTTTTGTACAGCAACAGGAGGGA4970.133004346057505No Hit
TCCCGATACAGAGCTGTAGCGGTATCTAGCAGATCTCGGACGGAAGGAAA4940.132201502922349No Hit
ATCCCAGAGTGAGAGGCCTGTATTTCCCTGCTGGTGGCTCCAGTTCAGGA4940.132201502922349No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTGCCGAGATGGAGATCT4920.13166627416557838No Hit
CAATAGGCCTGTTAACAGGGAGTTTTCTAAAACATTCTTTGATTTTGAGT4880.13059581665203707No Hit
GCACGGGACCCTGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG4880.13059581665203707No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA4850.12979297351688113No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA4780.12791967286818387No Hit
GGGTTAAAGATAGGTACAGTAGAAGAATAAAGTCCCGTAAGGTTTCCCAC4750.1271168297330279No Hit
GTACAAAACCTTTGGATGGAAACTGCACCTGTATTCCCATCCCATCATCC4720.1263139865978719No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCCG4720.1263139865978719No Hit
CTACTGTACCTATCTTTAACCCTCATTGGAAAACACCTTCTTTTCCTAAT4690.12551114346271597No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAG4680.12524352908433065No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTCGTTTA4640.12417307157078934No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4610.1233702284356334No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTTTCGCCAACTTACA4580.12256738530047742No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGGCATGGACATTGA4560.12203215654370678No Hit
GTACCTATCTTTAACCCTCATTGGAAAACACCTTCTTTTCCTAATATACA4520.12096169903016549No Hit
GTCCAAGGAATACTAACATTGAGATTGCCGAGATGGAGATCTTCTGCGAC4410.11801794086792694No Hit
GGCCTACAAACTGTTCACATTTTTTGATAATATCTTGGTGTAAATGTATA4330.11587702584084436No Hit
CTAGCTACCTGGGTGGGTACTAATTTAGATGATCAAGCATCCAGGGACCT4290.11480656832730309No Hit
TCCAGGGACCTAGTAGTCAGTTATGTCAACACTCATATGGGCCTAAAGTT4280.11453895394891776No Hit
CCCCAAAAGGCCTCCGTGCGGCGGGGTGAACCCTAGCCCGAATGCTCCAG4270.11427133957053245No Hit
ATATAAAACACCCGCCTTCCATAGCGTGTGTAAATAGTGTCTGGTTTGGA4210.1126656533002205No Hit
GGATAATAAGGTTTAATACCCTTGTCCAAGGGCAAATATTTGGTAGACTT4200.11239803892183518No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAATCTAGTCTCCTCC4190.11213042454344987No Hit
GATATGATCTTGTGGCAATGATCCCCAACTTCCAATGACATAACCCATGA4170.11159519578667922No Hit
GTCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGG4170.11159519578667922No Hit
GATAGGTACAGTAGAAGAATAAAGTCCCGTAAGGTTTCCCACCTTATGAG4130.11052473827313793No Hit
CTCTTTACGCGGCCTCCCCGTCTGTGCCTTTTCATCTGCCGGTCCGTGTG4120.11025712389475262No Hit
GAATAAAGTCCCGTAAGGTTTCCCACCTTATGAGTCCAAGGAATACTAAC4090.10945428075959665No Hit
ATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATTCAATCTAAGC4090.10945428075959665No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC4070.108919052002826No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTACTGGGGGC4020.10758098011089941No Hit
ATGTATATTTGCGGGAGAGAACAACGGAGTTGTCAGTCCCGAGAATGTTT3970.10624290821897278No Hit
GTTTGGTGGAAGGTTGTGGAGTTCCACTGCATGGCCTGAGGATGAGTGTT3950.10570767946220215No Hit
CTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC3930.10517245070543149No Hit
CATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGC3910.10463722194866086No Hit
GTTTTATATAAGAGAGAATCAACACATAGCGCCTCATTTTGTGGGTCACC3900.10436960757027552No Hit
GAATAACCCCATCTCTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGA3860.10329915005673423No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG3850.10303153567834893No Hit
GACATAACCCATGAAATTTAGAGAATAACCCCATCTCTTTGTTTTGTTAG3810.10196107816480764No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC3810.10196107816480764No Hit
CCCCTATCTTATCAACACTTCCGGAGACTACTGTTGTTAGACGACGAGGC3760.10062300627288104No Hit
GCCTGGGACCCTGTCGTCCCCTTCTCTGCCTGCCGTTCCGTCCGACCACG3760.10062300627288104No Hit
CAGTTATATGGATGATGTGGTACTGGGGGCCAAGTCTGTACAGCATCTTG3750.10035539189449572No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)