FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences408036
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25600.6273956219549255No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT14500.35536080149790705No Hit
CCCTTACAGTAAATGAAAAAAGGAGATTGAAATTAATTATGCCTGCTAGG14200.34800850905312275No Hit
GTTCAAATACTGTTGACGTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA13520.33134331284494506No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA12090.2962973855248066No Hit
GGGAAGCAGTAGTCAGCTATGTCAACGTTAATATGGGCATAAAAATCAGA8320.20390357713535082No Hit
CTGTCAGGGGCCCCACAAATTGCTCACATCTATCAACAATGTCCTCCTGC7890.19336529129782667No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT7780.1906694507347391No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT7650.18748345734199923No Hit
GTATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCTGGTCTGTGCCAAG7550.1850326931937378No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACCCCCAATTACATATCCCATGAA7100.17400425452656137No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCCAGGGGGAACACCCGTG6890.16885764981521237No Hit
GTTCAGATACTGTTGACGTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA6840.16763226774108167No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAAGGCAAATATTTAGTAACATT6840.16763226774108167No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC6750.1654265800076464No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC6610.16199551020008038No Hit
TCCTTCATCCAGGGAAGCAGTAGTCAGCTATGTCAACGTTAATATGGGCA6520.1597898224666451No Hit
CTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTC6470.15856444039251438No Hit
CATTTTGATTTTTTGTACAATATGTTCCTGTGGCAATGTGCCCCAACCCC6290.15415306492564382No Hit
CTGTAAGGGGCCCCACAAATTGCTCACATCTATCAACAATGTCCTCCTGC6080.14900646021429484No Hit
CCCTGACAGTAAATGAAAAAAGGAGATTGAAATTAATTATGCCTGCTAGG5950.14582046682155494No Hit
ATCAAACTGTGTTTCAGAAAACTTCCTGTAAACAGACCTATTGATTGGAA5930.1453303139919027No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT5810.14238939701398898No Hit
GTCTTGAAGTAATGATTAACTACATGCTCTGGATAATAAGGTTTGATCCC5810.14238939701398898No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT5790.1418992441843367No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTGAAA5770.14140909135468438No Hit
AAACAAAAAGATGGGGATATTCCCTTAACTTCATGGGATATGTAATTGGG5680.1392034036212491No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA5540.13577233381368312No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA5350.1311158819319864No Hit
ATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGTCTGGA5300.1298904998578557No Hit
GTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT5280.1294003470282034No Hit
ATATTTACCTTTAGATAAAGGGATCAAACCTTATTATCCAGAGCATGTAG5180.12694958287994199No Hit
ACGTTAATATGGGCATAAAAATCAGACAACTATTGTGGTTTCACATTTCC5020.12302836024272368No Hit
GACCTGCTGCGAGCAAAACAAGCGGCTAGGAGTTCCGCAGTATGGATCGG4980.12204805458341911No Hit
AGATAAAGGGATCAAACCTTATTATCCAGAGCATGTAGTTAATCATTACT4930.1208226725092884No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC4890.11984236684998383No Hit
ACCTCAACCCGCACAAGGACAACTGGCCGGACGCCAACAAGGTGGGAGTG4850.11886206119067927No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT4840.11861698477585311No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCTTTAGATAAAGGGATCAAAC4760.11665637345724397No Hit
CCTGTAAACAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT4740.11616622062759169No Hit
GGTTTAGGTCCCAATCTGGATTTTCTGAGTTGGCTTTGAATGCAGGGTCC4740.11616622062759169No Hit
GTAGTCAGCTATGTCAACGTTAATATGGGCATAAAAATCAGACAACTATT4730.11592114421276555No Hit
CGTTAATATGGGCATAAAAATCAGACAACTATTGTGGTTTCACATTTCCT4630.1134703800645041No Hit
ACATAAGACAGGAAATGTGAAACCACAATAGTTGTCTGATTTTTATGCCC4580.11224499799037341No Hit
GTATTTGAACCTTTACCCCGTTGCTCGGCAACGGTCTGGTCTGTGCCAAG4580.11224499799037341No Hit
ATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATAAGTTTT4570.11199992157554725No Hit
TATCCACATTGTGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG4470.10954915742728583No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA4430.10856885176798127No Hit
GTGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCGTTGACATACT4310.10562793479006755No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAACATAAGACA4300.10538285837524139No Hit
CACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGGTACCTTGC4290.10513778196041526No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAACATAAGA4280.10489270554558912No Hit
CCTGACTGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAGGAGGACAT4280.10489270554558912No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC4220.10342224705663226No Hit
GTTAGTATTCCATGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTC4190.10268701781215384No Hit
CCTTTAGATAAAGGGATCAAACCTTATTATCCAGAGCATGTAGTTAATCA4150.10170671215284925No Hit
ATATTTGCCTTTAGATAAAGGGATCAAACCTTATTATCCAGAGCATGTAG4110.1007264064935447No Hit
GAATAAAGCCCCGTAAAGTTTCCCACCTTATGTGTCCATGGAATACTAAC4100.10048133007871855No Hit
ATGTAATTGGGGGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA4090.1002362536638924No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)