FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490507

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490507
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences408036
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25390.6222490172435765No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT13810.3384505288749032No Hit
GTTCAAATACTGTTGACGTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA13350.3271770137929006No Hit
CCCTTACAGTAAATGAAAAAAGGAGATTGAAATTAATTATGCCTGCTAGG13090.3208050270074209No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA10850.26590791008636494No Hit
CTGTCAGGGGCCCCACAAATTGCTCACATCTATCAACAATGTCCTCCTGC9150.22424491956592063No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT7740.18968914507543452No Hit
GTATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCTGGTCTGTGCCAAG7640.18723838092717307No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACCCCCAATTACATATCCCATGAA7220.17694517150447509No Hit
TCCTTCATCCAGGGAAGCAGTAGTCAGCTATGTCAACGTTAATATGGGCA7080.1735141016969091No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCCAGGGGGAACACCCGTG6940.1700830318893431No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC6910.16934780264486465No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT6900.16910272623003852No Hit
CTGTAAGGGGCCCCACAAATTGCTCACATCTATCAACAATGTCCTCCTGC6770.16591673283729866No Hit
GTTCAGATACTGTTGACGTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA6620.1622405866149065No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAAGGCAAATATTTAGTAACATT6340.15537844699977452No Hit
CATTTTGATTTTTTGTACAATATGTTCCTGTGGCAATGTGCCCCAACCCC5920.14508523757707653No Hit
CTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTC5690.13944848003607527No Hit
CCCTGACAGTAAATGAAAAAAGGAGATTGAAATTAATTATGCCTGCTAGG5650.13846817437677067No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTGAAA5620.13773294513229226No Hit
ATCAAACTGTGTTTCAGAAAACTTCCTGTAAACAGACCTATTGATTGGAA5610.1374878687174661No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA5510.1350371045692047No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT5460.13381172249507398No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA5450.13356664608024782No Hit
GTCTTGAAGTAATGATTAACTACATGCTCTGGATAATAAGGTTTGATCCC5270.12915527061337725No Hit
ATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGTCTGGA5250.12866511778372497No Hit
AGATAAAGGGATCAAACCTTATTATCCAGAGCATGTAGTTAATCATTACT5240.12842004136889884No Hit
AAACAAAAAGATGGGGATATTCCCTTAACTTCATGGGATATGTAATTGGG5240.12842004136889884No Hit
GGGAAGCAGTAGTCAGCTATGTCAACGTTAATATGGGCATAAAAATCAGA5200.12743973570959424No Hit
GTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT5170.12670450646511583No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT5130.12572420080581126No Hit
ACCTTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATT5120.12547912439098513No Hit
GTATTTGAACCTTTACCCCGTTGCTCGGCAACGGTCTGGTCTGTGCCAAG4930.1208226725092884No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAACATAAGA4930.1208226725092884No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT4870.11935221402033154No Hit
ATATTTACCTTTAGATAAAGGGATCAAACCTTATTATCCAGAGCATGTAG4830.11837190836102697No Hit
GTGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCGTTGACATACT4730.11592114421276555No Hit
GTAGTCAGCTATGTCAACGTTAATATGGGCATAAAAATCAGACAACTATT4710.11543099138311326No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC4680.11469576213863483No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAACATAAGACA4610.11298022723485183No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCTTTAGATAAAGGGATCAAAC4480.10979423384211197No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC4480.10979423384211197No Hit
ATCTACAGCATGGGAGGTTGGTCTTCCAAACCTCGAAAAGGCATGGGGAC4360.10685331686419826No Hit
GGATATCCACATTGTGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT4340.10636316403454599No Hit
ATATTTGCCTTTAGATAAAGGGATCAAACCTTATTATCCAGAGCATGTAG4330.10611808761971983No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC4300.10538285837524139No Hit
CGTTAATATGGGCATAAAAATCAGACAACTATTGTGGTTTCACATTTCCT4280.10489270554558912No Hit
CCTGTAAACAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT4270.10464762913076296No Hit
GGTTTAGGTCCCAATCTGGATTTTCTGAGTTGGCTTTGAATGCAGGGTCC4270.10464762913076296No Hit
ACATAAGACAGGAAATGTGAAACCACAATAGTTGTCTGATTTTTATGCCC4250.10415747630111069No Hit
TATCCACATTGTGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG4200.10293209422697998No Hit
ACCTCAACCCGCACAAGGACAACTGGCCGGACGCCAACAAGGTGGGAGTG4200.10293209422697998No Hit
CCTGACTGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAGGAGGACAT4200.10293209422697998No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA4190.10268701781215384No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG4120.10097148290837082No Hit
ACGTTAATATGGGCATAAAAATCAGACAACTATTGTGGTTTCACATTTCC4120.10097148290837082No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)