FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002490508

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490508
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences580906
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC19110.32896888653241657No Hit
GGATAATAGGGTTTGATTCCTTTATCTAAGGGCAAGTATTTGGTAACCTT18710.3220830908959453No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC17860.30745077516844377No Hit
GGACATGTGAAACCACAATAGTTGTCTGATTTTTAGGCCCATATTAACAT17860.30745077516844377No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAA16730.2879984024954123No Hit
TCCAAAGAGTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA16110.2773254192588818No Hit
GTCTTTGAAGTATGCTTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA15720.2706117685133223No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGT14970.2577009016949386No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTCTCTAGGGGAAAAACCCGTG14910.2566680323494679No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGATA13960.24031426771284858No Hit
AATCCGGAGACTAAAATTAATTATGCCTGCTAGATTTTATCCCAAGGTTA13790.23738780456734826No Hit
TATCCACATTGTGTAAAAGGGGCAGCGAATCCCAAAAGACCCACAATTCG12860.22137832971255247No Hit
GTGTAAAAGGGGCAGCGAATCCCAAAAGACCCACAATTCGTTGACAAACT12610.2170747074397579No Hit
GTTGTGAGTATGCCCTGAGTCTGAGGGCTCCACCCCAACAGGCCCCCATG12060.20760673843960983No Hit
GTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCGGG11570.1991716387849325No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11330.19504016140304972No Hit
ACTCACAACTGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT10950.18849865554840198No Hit
ATCTAAGGGCAAGTATTTGGTAACCTTGGGATAAAATCTAGCAGGCATAA10940.1883265106574902No Hit
ATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGTCTGGA10600.18247358436648958No Hit
GCACCGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTTTG10590.1823014394755778No Hit
GTAAAAGCCTGTTTAGCTTGTATACATGCATATAAAGGCATTAAAGCAGG10470.1802357007846364No Hit
GCCTAGCAGCCATGGAAAAGATGTATACTTGCGGGAGAGCACGACAGAAT10240.17627636829366541No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC10220.17593207851184184No Hit
CTATTATCCCGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT10160.17489920916637117No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT10140.1745549193845476No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC10080.1735220500390769No Hit
GTTTCGTTTTGTGAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTG10050.17300561536634154No Hit
ATATGTTCCTGTGGCAAGGAACCCCAACTTCCAATTACATATCCCATGAA9400.16181619745707568No Hit
CACCTTTCCTGTCTTATGTTTGGACAAGATACTGTTCTTGAATATTTGGT9190.15820115474792823No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG9130.15716828540245756No Hit
TTTGCAAGATCCAGCATCCAGGGAAGCAGTAGTCAATTATGTCAATGTTA9070.15613541605698683No Hit
CTCCTGATTTCATTTACTGTTAGGGGTCCCACAAATTGCTCACATCTATC8740.150454634656898No Hit
AAACAAAACGATGGGGATATTCACTGAATTTCATGGGATATGTAATTGGA8590.1478724612932213No Hit
ACCAAATACTTGCCCTTAGATAAAGGAATCAAACCCTATTATCCCGAGCA8580.1477003164023095No Hit
GTATTTGGTAACCTTGGGATAAAATCTAGCAGGCATAATTAATTTTAGTC8380.14425741858407384No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGGTACCTAAC8360.14391312880225027No Hit
GTACAATATGTTCCTGTGGCAAGGAACCCCAACTTCCAATTACATATCCC8170.14064237587492642No Hit
CACATGTCCTGTATTATGTTTGGACAAGATACTGTTCTTGAATTTTTGGT8120.1397816514203675No Hit
GTCCGGTGCTGGTGGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC8000.1377159127294261No Hit
GTTATTAGACAACGAAGCAGGTCCACTAGAAGAAGAACTCCCTCGCCTCG7930.13651089849304363No Hit
CTTTAATCCTGATTGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAGG7930.13651089849304363No Hit
GGAAAGGTGAAACCACAATAGTTGTCTGATTTTTAGGCCCATATTAACAT7740.13324014556571975No Hit
GGATATCCACATTGTGTAAAAGGGGCAGCGAATCCCAAAAGACCCACAAT7660.13186298643842548No Hit
GCTTTATTCTTCTACGGTACCTAACTTTAATCCTGATTGGCAAACTCCTT7550.12996939263839588No Hit
CATCTATCAATAATGTCTTCCTGCAAATGAATGTCAGGAAAAGAAGGAGT7520.12945295796566056No Hit
CCCTTAGATAAAGGAATCAAACCCTATTATCCCGAGCATGTAGTTAATCA7520.12945295796566056No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG7470.12859223351110163No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAAGGGGCAGCG7440.12807579883836626No Hit
CTCCAAAAGATACCAAAAATTCAAGAACAGTATCTTGTCCAAACATAATA7420.1277315090565427No Hit
ACGTACAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCAC7310.1258379152565131No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACATCACCATACGGCACTCAGGCA7270.125149335692866No Hit
ACCTAACTTTAATCCTGATTGGCAAACTCCTTCTTTTCCTGACATTCATT7110.12239501743827744No Hit
GGGTAAAGGTTCAGATACTGGTTACGTAAAAAGGCCTTGTAAGTTGGCGA7100.12222287254736568No Hit
GTAGTCAATTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT7070.12170643787463031No Hit
CCTTTATCTAAGGGCAAGTATTTGGTAACCTTGGGATAAAATCTAGCAGG7050.12136214809280675No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA7020.12084571342007142No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC6940.11946855429277715No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATGTCC6930.11929640940186537No Hit
ACATAATACAGGACATGTGAAACCACAATAGTTGTCTGATTTTTAGGCCC6920.11912426451095358No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA6890.11860782983821824No Hit
GTAAACACACAGTCTTTGAAGTATGCTTCAAGGTCGGTCGTTGACATTGC6890.11860782983821824No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT6810.11723067071092398No Hit
GCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATAGGGGAGTAGGCTGCCT6780.11671423603818862No Hit
CTTTTACACAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA6760.11636994625636506No Hit
GAGTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCG6730.11585351158362972No Hit
CACCTTTCCTGTCTTATGTTTGGACAAGATACTGTTCTTGAATTTTTGGT6620.1139599177836001No Hit
CTGTTAGGGGTCCCACAAATTGCTCACATCTATCAATAATGTCTTCCTGC6600.11361562800177655No Hit
ACATTATTGATAGATGTGAGCAATTTGTGGGACCCCTAACAGTAAATGAA6600.11361562800177655No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAAC6570.11309919332904118No Hit
GTATTATGTTTGGACAAGATACTGTTCTTGAATTTTTGGTATCTTTTGGA6550.11275490354721762No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATAAGTTT6550.11275490354721762No Hit
ATTTGGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGATA6500.11189417909265871No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT6420.11051701996536445No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT6420.11051701996536445No Hit
CTTCTACGGTACCTAACTTTAATCCTGATTGGCAAACTCCTTCTTTTCCT6370.10965629551080552No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG6320.10879557105624661No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6290.10827913638351128No Hit
TTCGAGAGCTTCTCGACACCGCCACTGCTTTGTATCGGGAGGCCTTAGAG6250.10759055681986414No Hit
TTCCCTGAATTTCATGGGATATGTAATTGGAAGTTGGGGTTCCTTGCCAC6210.10690197725621702No Hit
CTTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGT6200.10672983236530523No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA5970.10277049987433423No Hit
TCCTCAGGCCGTGCGGTGGAACTCCACCACTTTCCATCAAACTCTTCAAG5960.10259835498342244No Hit
ATTCCCTGAATTTCATGGGATATGTAATTGGAAGTTGGGGTTCCTTGCCA5930.10208192031068711No Hit
GTCTAATAACAGTAGTTTCCGGAAGTGTTGATAAGATAGGGGCATTTGGT5930.10208192031068711No Hit
GTCCTGATGCGATGTTCTCCATGTTCGGTACAGGGTCCCCAGTCGTCGAG5920.10190977541977532No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACCACC5910.10173763052886353No Hit
GATCCAGCATCCAGGGAAGCAGTAGTCAATTATGTCAATGTTAATATGGG5890.10139334074703997No Hit
GTATGGATCGGCAGCGGAGACACAAAGGTTCCGCGCATGCGCTGATGGCC5880.10122119585612818No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTTTGTCAACGAATTGTGGGTCT5840.10053261629248106No Hit
ATGTAAGACCTTGGGCAGGTTCCGGTGGGCGTTCACGGTGGTCTCCATGC5840.10053261629248106No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)