FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002490509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences580906
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAATAGGGTTTGATTCCTTTATCTAAGGGCAAGTATTTGGTAACCTT18420.3170908890595036No Hit
GGACATGTGAAACCACAATAGTTGTCTGATTTTTAGGCCCATATTAACAT17250.29694993682282506No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC16670.28696553314994167No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTCTCTAGGGGAAAAACCCGTG16290.28042402729529387No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC15940.2743989561133815No Hit
TCCAAAGAGTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA14300.24616719400384915No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAA14200.24444574509473135No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGATA13570.233600616967289No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGT13560.23342847207637724No Hit
GTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCGGG13310.22912484980358266No Hit
GTGTAAAAGGGGCAGCGAATCCCAAAAGACCCACAATTCGTTGACAAACT13130.22602624176717062No Hit
GTCTTTGAAGTATGCTTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA12860.22137832971255247No Hit
AATCCGGAGACTAAAATTAATTATGCCTGCTAGATTTTATCCCAAGGTTA11490.19779447965763822No Hit
GTTGTGAGTATGCCCTGAGTCTGAGGGCTCCACCCCAACAGGCCCCCATG11420.19658946542125574No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11240.19349085738484367No Hit
CTATTATCCCGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT10770.18540004751198988No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT10360.1783421069846068No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC10120.17421062960272402No Hit
ATCTAAGGGCAAGTATTTGGTAACCTTGGGATAAAATCTAGCAGGCATAA10060.17317776025725332No Hit
GTATTTGGTAACCTTGGGATAAAATCTAGCAGGCATAATTAATTTTAGTC9750.16784126863898807No Hit
ATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGTCTGGA9690.16680839929351737No Hit
GTTTCGTTTTGTGAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTG9630.16577552994804667No Hit
TATCCACATTGTGTAAAAGGGGCAGCGAATCCCAAAAGACCCACAATTCG9460.16284906680254638No Hit
CTCCTGATTTCATTTACTGTTAGGGGTCCCACAAATTGCTCACATCTATC9380.1614719076752521No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC9370.16129976278434033No Hit
GCACCGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTTTG9140.1573404302933693No Hit
ACTCACAACTGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT9110.15682399562063398No Hit
GTACAATATGTTCCTGTGGCAAGGAACCCCAACTTCCAATTACATATCCC9100.15665185072972218No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG9040.1556189813842515No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTTTGTCAACGAATTGTGGGTCT8460.14563457771136812No Hit
GTAAAAGCCTGTTTAGCTTGTATACATGCATATAAAGGCATTAAAGCAGG8440.14529028792954454No Hit
CTTTAATCCTGATTGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAGG8320.14322454923860314No Hit
ATATGTTCCTGTGGCAAGGAACCCCAACTTCCAATTACATATCCCATGAA8120.1397816514203675No Hit
TTTGCAAGATCCAGCATCCAGGGAAGCAGTAGTCAATTATGTCAATGTTA8070.13892092696580857No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC8050.13857663718398502No Hit
ACCAAATACTTGCCCTTAGATAAAGGAATCAAACCCTATTATCCCGAGCA7930.13651089849304363No Hit
ATTTGGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGATA7840.13496159447483758No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGGTACCTAAC7700.13255156600207263No Hit
GCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATAGGGGAGTAGGCTGCCT7610.13100226198386658No Hit
CTTTTACACAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA7510.12928081307474876No Hit
CTCCAAAAGATACCAAAAATTCAAGAACAGTATCTTGTCCAAACATAATA7480.1287643784020134No Hit
GTTATTAGACAACGAAGCAGGTCCACTAGAAGAAGAACTCCCTCGCCTCG7460.12842008862018983No Hit
GCTTTATTCTTCTACGGTACCTAACTTTAATCCTGATTGGCAAACTCCTT7270.125149335692866No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACATCACCATACGGCACTCAGGCA7260.1249771908019542No Hit
CCCTTAGATAAAGGAATCAAACCCTATTATCCCGAGCATGTAGTTAATCA7190.1237721765655717No Hit
GTCCGGTGCTGGTGGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC7180.12360003167465994No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA7160.12325574189283636No Hit
GTATTATGTTTGGACAAGATACTGTTCTTGAATTTTTGGTATCTTTTGGA7060.12153429298371854No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAAC6980.12015713385642429No Hit
GGGTAAAGGTTCAGATACTGGTTACGTAAAAAGGCCTTGTAAGTTGGCGA6960.11981284407460072No Hit
CACCTTTCCTGTCTTATGTTTGGACAAGATACTGTTCTTGAATATTTGGT6880.11843568494730645No Hit
CCTTTATCTAAGGGCAAGTATTTGGTAACCTTGGGATAAAATCTAGCAGG6860.11809139516548288No Hit
GGAAAGGTGAAACCACAATAGTTGTCTGATTTTTAGGCCCATATTAACAT6790.1168863809291004No Hit
TTCCCTGAATTTCATGGGATATGTAATTGGAAGTTGGGGTTCCTTGCCAC6690.11516493201998258No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG6640.11430420756542367No Hit
GTAGTCAATTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT6620.1139599177836001No Hit
TCCTCAGGCCGTGCGGTGGAACTCCACCACTTTCCATCAAACTCTTCAAG6620.1139599177836001No Hit
GGCCTTTACTTTCTCGCCAACTTACAAGGCCTTTTTACGTAACCAGTATC6600.11361562800177655No Hit
GTCTAATAACAGTAGTTTCCGGAAGTGTTGATAAGATAGGGGCATTTGGT6590.11344348311086476No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT6560.11292704843812941No Hit
ACCTAACTTTAATCCTGATTGGCAAACTCCTTCTTTTCCTGACATTCATT6490.11172203420174692No Hit
CTGTTAGGGGTCCCACAAATTGCTCACATCTATCAATAATGTCTTCCTGC6490.11172203420174692No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGAGGGACGTCCTTTGTTTA6450.11103345463809978No Hit
ACATTATTGATAGATGTGAGCAATTTGTGGGACCCCTAACAGTAAATGAA6440.11086130974718802No Hit
CACATGTCCTGTATTATGTTTGGACAAGATACTGTTCTTGAATTTTTGGT6410.11034487507445266No Hit
ACATAATACAGGACATGTGAAACCACAATAGTTGTCTGATTTTTAGGCCC6380.10982844040171731No Hit
AAACAAAACGATGGGGATATTCACTGAATTTCATGGGATATGTAATTGGA6360.10948415061989376No Hit
CATCTATCAATAATGTCTTCCTGCAAATGAATGTCAGGAAAAGAAGGAGT6350.10931200572898198No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATGTCC6290.10827913638351128No Hit
GAGTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCG6280.10810699149259947No Hit
CTGCCGTACCGTCCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCCC6220.1070741221471288No Hit
GGATATCCACATTGTGTAAAAGGGGCAGCGAATCCCAAAAGACCCACAAT6190.10655768747439345No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA6180.10638554258348167No Hit
CTTCTACGGTACCTAACTTTAATCCTGATTGGCAAACTCCTTCTTTTCCT6140.10569696301983454No Hit
GTACCTAACTTTAATCCTGATTGGCAAACTCCTTCTTTTCCTGACATTCA6130.10552481812892275No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG6080.10466409367436383No Hit
GTCCTGATGCGATGTTCTCCATGTTCGGTACAGGGTCCCCAGTCGTCGAG6060.10431980389254028No Hit
GTAAACACACAGTCTTTGAAGTATGCTTCAAGGTCGGTCGTTGACATTGC5860.10087690607430463No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)