FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences499640
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26410.5285805780161716No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT17820.3566567928908815No Hit
ATATGAAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA17810.35645664878712674No Hit
CATTACCACAGGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAA16890.338043391241694No Hit
GTGCTGGTGAACGCACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC15520.31062364902729966No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC13320.2665919462012649No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA13260.26539108157873664No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAACACCCGTG13140.26298935233368026No Hit
CCCTTACAGTAAATGAAAAAAGGAGACTAAAATTAATTATGCCTGCTAGG12640.2529821471459451No Hit
GTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAATCCTCACAAAAC12070.241573933231927No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA11570.23156672804419184No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACCTT11510.2303658634216636No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT11160.22336081979024897No Hit
GTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAAGTTAAGGGAA11040.22095909054519253No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA10690.2139540469137779No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA9930.19874309502842047No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT9590.19193819550076055No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC9580.19173805139700584No Hit
ATACCGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTAATGAATCTA9480.18973661035945882No Hit
GACGCAAACTGCACATGTATTCCCATCCCATCATCTTGGGCTTTCGCAAA9460.1893363221519494No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG9130.1827315667280442No Hit
GTGGTAATGTGCCCCAACTCCCAATTACATATCCCATGAAGTTAAGGGAA9050.18113041389800658No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT8790.17592666720038427No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT8320.1665198943239132No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGTGCTCCACCCCAACAGTCCCCCATG8120.16251701224881915No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGAT7700.1541109598911216No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT7680.15371067168361222No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC7370.1475062044672164No Hit
CATTTAAATCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT7320.14650548394844287No Hit
CCTTCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGAAAC7300.14610519574093347No Hit
ATACGGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTAATGAATCTA7010.14030101673204706No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT7010.14030101673204706No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA6970.13950044031702827No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA6960.13930029621327356No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC6950.13910015210951884No Hit
GCACCGGACCATGCAAAACCTGCACAACGCCTGCTCAAGGAACCTCTATG6880.13769914338323594No Hit
GTTGATAGATGTGAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAAAAG6610.13229525258185895No Hit
TGATAGATGTGAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAAAAGGA6560.13129453206308542No Hit
CCAATCAATAGGCCGGTTTACAGGAAGTTTCCTAAAACACATTTTGATTT6520.1304939556480666No Hit
GGGTAAAGGTTCAGATACTGTTTACGTAGAAAGGCCTTGTAAGTTGGCGA6400.12809222640301016No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT6400.12809222640301016No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA6180.1236890561204067No Hit
ATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACAC6140.12288847970538788No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAGCAG6130.12268833560163317No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT6120.12248819149787847No Hit
CATGTATACAAGCAAAGCAGGCTTTTACTTTCTCGCCAACTTACAAGGCC6000.12008646225282202No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6000.12008646225282202No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC5970.11948602994155792No Hit
CTGTAAGGGGCCCCACAAATTGCTCACATCTATCAACAATGTCCTCCTGC5960.11928588583780322No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT5950.11908574173404851No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC5820.11648386838523736No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG5770.11548314786646385No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA5760.11528300376270914No Hit
TCCCGATACAGAGCAGAAGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA5670.11348170682891681No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5660.11328156272516213No Hit
CATTGCCACAGGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAA5620.1124809863101433No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAGC5600.1120806981026339No Hit
CCTCCTTCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA5570.1114802657913698No Hit
GTTTTATCCCAAGGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC5560.11128012168761509No Hit
CATCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGT5520.11047954527259626No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGACA5470.10947882475382276No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAAGCGGTGTCGAGGAGATCTCGAA5470.10947882475382276No Hit
GAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGACA5420.10847810423504924No Hit
CTTCAAAGACTGTGTGTTTACTGAGTGGGAGGAGTTCGGGGAGGAGGTTA5410.10827796013129452No Hit
GTGTTGGGGTGAGTTAATGAATCTAGCTACCTGGGTGGGAAGTAATTTGG5410.10827796013129452No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG5350.10707709550876632No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGACATG5280.10567608678248339No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC5220.10447522215995518No Hit
GTTCAGATACTGTTTACGTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA5220.10447522215995518No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5210.10427507805620047No Hit
ATTCTGTGTTGGGGTGAGTTAATGAATCTAGCTACCTGGGTGGGAAGTAA5130.10267392522616282No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC5120.10247378112240814No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT5120.10247378112240814No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5120.10247378112240814No Hit
ACACCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGG5110.10227363701865343No Hit
GTGCCGTATGGTGAGGTGAACAATGTTCCGGAGACTCTAAGGCCTCCCGA5100.10207349291489873No Hit
GTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGG5050.10107277239612522No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)