FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490512

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490512
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences532670
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN30900.5800964950156757No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA17780.3337901515009293No Hit
ACTCACAACTGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT14830.2784087709088179No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC13850.2600108885426249No Hit
GTTCTTACACCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAG12110.22734526066795577No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAAGCCCCCATG11960.22452925826496706No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG11610.21795858599132673No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC11410.21420391612067508No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGT10830.20331537349578538No Hit
GTGTAAGAACTTTGTGGGACCCCTAACAGTAAATGAAAACAGGAGATTAA10050.18867216100024406No Hit
ATCTAAGGGCAAGTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAA9750.1830401561942666No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTATCTCTTCCAAAAGTAAGA9370.17590628344002854No Hit
GCTCTATATCGAGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA8950.16802147671166012No Hit
GTGTAAAAGGGGCAGCGAACCCCAAAAGACCCACAATTCGTTGACACACC8800.1652054743086714No Hit
GTAGTAAGCTATGTCAATGTTAACATGGGCCTAAAAATCAGACAACTATT8790.1650177408151388No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA8350.15675746709970526No Hit
GGATAATGTGGTTTGATTCCTTTATCTAAGGGCAAGTATTTGGTAACATT8240.15469239867084686No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC8190.15375373120318397No Hit
GTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAATTAATTTTAATC8150.15300279722905363No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAATTTCAT8140.15281506373552106No Hit
ATATGTTCCTGTGGCAAGGAGCCCCAACTCCCAATGACATATCCCATGAA8010.1503745283195975No Hit
GTCCGGTGCTGGTGGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC8000.1501867948260649No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG7700.1445547900200875No Hit
TCCTCAGGCCATGCAGTGGAACACCACCACTTTCCATCAAACTCTACAAG7540.14155105412356617No Hit
ATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATTTACAT7510.14098785364296842No Hit
GTATACATGCATATAAAGGCATTAAAGCGGGATATCCACATTGTGTAAAA7310.13723318377231683No Hit
ATATAGAGCTGAGGCGGTGTCGAGAAGGTCTCGAATAGAAGGAAAGAAGT7300.13704545027878423No Hit
TTCAAGAACAGTATCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA7220.1355435823305236No Hit
ATATAAAAGAGAGACAACACGTAGCGCCTCATTTTGCGGGTCACCATATT7200.13516811534345843No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7140.13404171438226295No Hit
GTTGATAGGTGTAAGAACTTTGTGGGACCCCTAACAGTAAATGAAAACAG7040.13216437944693712No Hit
ACCCAGCATCCAGGGAATTAGTAGTAAGCTATGTCAATGTTAACATGGGC7030.13197664595340455No Hit
CCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT7020.13178891245987195No Hit
TCCAAAGAGTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA6980.13103797848574164No Hit
ACCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGCACGACAGAAT6900.129536110537481No Hit
CTTTAATCCTGATTGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAGG6880.12916064355041584No Hit
GCACCGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG6800.1276587756021552No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA6690.1255937071732968No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTTCTGTACCTAAC6660.12503050669269905No Hit
ATGTTAACATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC6650.12484277319916647No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG6590.12371637223797098No Hit
CAAGATCTACAGCATGGGAGGTTGGTCTTCAAAACCTCGAAAAGGCATGG6550.12296543826384065No Hit
GCTTTATTCTTCTTCTGTACCTAACTTTAATCCTGATTGGCAAACTCCTT6510.12221450428971031No Hit
CTGTTAGGGGTCCCACAAAGTTCTTACACCTATCAACAATGTCCTCCTGC6470.12146357031558No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGACAACACGTAGCGCC6450.12108810332851483No Hit
CCCTAACAGTAAATGAAAACAGGAGATTAAAATTAATTATGCCTGCTAGA6420.12052490284791709No Hit
CTTTTACACAATGTGGATATCCCGCTTTAATGCCTTTATATGCATGTATA6310.1184598344190587No Hit
ATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGTCTGGA6250.11733343345786322No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACACCACCACTTT6240.11714569996433062No Hit
GTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGG6190.11620703249666774No Hit
GCTTACTACTAATTCCCTGGATGCTGGGTCGTCCAAATTACTTCCCACCC6120.11489289804193967No Hit
ATGCTGGGTCGTCCAAATTACTTCCCACCCAGGTGGCTAGATTCATCAAC6120.11489289804193967No Hit
GGATAATATGGTTTGATTCCTTTATCTAAGGGCAAGTATTTGGTAACATT6120.11489289804193967No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCGGTG6090.11432969756134193No Hit
CCCAATGACATATCCCATGAAATTAAGGGAATATCCCCATCTTTTTGTTT6080.11414196406780933No Hit
ACATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT6080.11414196406780933No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT6060.11376649708074418No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTTCTG6040.11339103009367901No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG6010.11282782961308127No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6000.1126400961195487No Hit
CTCTTATGCAAGACCTTGGGCAGGTTCCGGTGGGCGTTCACGGTGGTCTC5990.1124523626260161No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACCACC5970.11207689563895096No Hit
CTCCTAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGACCCGTAT5880.11038729419715772No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGTGGATTGGGGACAGAAAGAT5810.10907315974242963No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA5750.10794675878123415No Hit
GCTCTGGATAATGTGGTTTGATTCCTTTATCTAAGGGCAAGTATTTGGTA5730.10757129179416901No Hit
CCAAAAGACACCAAATATTCAAGAACAGTATCTCTTCCAAAAGTAAGACA5730.10757129179416901No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5710.10719582480710384No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACAAAATGCC5690.10682035782003867No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCTCTCAGGCA5690.10682035782003867No Hit
CCTTTATCTAAGGGCAAGTATTTGGTAACATTGGGATAAAATCTAGCAGG5690.10682035782003867No Hit
GGAACATATTGTACAAAAAATCAAATTATGTTTTAGGAAACTTCCTGTAA5680.1066326243265061No Hit
GCTCAGAATACTGCCTCTGCCATATCGTCAACCTTCTCGAAGACTGGGGA5660.10625715733944092No Hit
ACCCCTAACAGTAAATGAAAACAGGAGATTAAAATTAATTATGCCTGCTA5650.10606942384590835No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC5470.10269022096232189No Hit
GTTTACAGGAAGTTTCCTAAAACATAATTTGATTTTTTGTACAATATGTT5400.10137608650759382No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTCCCGCGCATGCGCTGATGGCC5400.10137608650759382No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGATACTGTTCT5360.1006251525334635No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)