FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490513

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490513
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences532670
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN30660.5755908911708938No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA15340.2879831790789795No Hit
ACTCACAACTGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT13720.25757035312670135No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC13000.24405354159235548No Hit
GTTCTTACACCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAG11950.22434152477143446No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG11800.22152552236844575No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAAGCCCCCATG11430.21457938310774027No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGT10770.2021889725345899No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC10400.1952428332738844No Hit
GTGTAAGAACTTTGTGGGACCCCTAACAGTAAATGAAAACAGGAGATTAA9740.18285242270073404No Hit
ATCTAAGGGCAAGTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAA9370.17590628344002854No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTATCTCTTCCAAAAGTAAGA9120.171212946101714No Hit
GTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAATTAATTTTAATC9080.1704620121275837No Hit
GTAGTAAGCTATGTCAATGTTAACATGGGCCTAAAAATCAGACAACTATT8960.1682092102051927No Hit
GCTCTATATCGAGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA8760.16445454033454107No Hit
GGATAATGTGGTTTGATTCCTTTATCTAAGGGCAAGTATTTGGTAACATT8640.16220173841215013No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA8610.1616385379315524No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAATTTCAT8420.1580716015544333No Hit
GTGTAAAAGGGGCAGCGAACCCCAAAAGACCCACAATTCGTTGACACACC7870.14774625941014136No Hit
TCCTCAGGCCATGCAGTGGAACACCACCACTTTCCATCAAACTCTACAAG7530.1413633206300336No Hit
ATATGTTCCTGTGGCAAGGAGCCCCAACTCCCAATGACATATCCCATGAA7530.1413633206300336No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC7530.1413633206300336No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA7330.13760865075938197No Hit
ATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATTTACAT7270.1364822497981865No Hit
CTTTAATCCTGATTGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAGG7130.13385398088873035No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7120.13366624739519778No Hit
TCCAAAGAGTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA7110.13347851390166518No Hit
GTCCGGTGCTGGTGGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC7050.1323521129404697No Hit
ACCCAGCATCCAGGGAATTAGTAGTAAGCTATGTCAATGTTAACATGGGC7040.13216437944693712No Hit
CCTGTAAACAGGCCCATTGATTGGAAGGTGTGTCAACGAATTGTGGGTCT7000.1314134454728068No Hit
ATATAAAAGAGAGACAACACGTAGCGCCTCATTTTGCGGGTCACCATATT6980.13103797848574164No Hit
CCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT6830.12822197608275293No Hit
TTCAAGAACAGTATCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA6830.12822197608275293No Hit
CTTTTACACAATGTGGATATCCCGCTTTAATGCCTTTATATGCATGTATA6820.12803424258922036No Hit
GTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGG6780.12728330861509002No Hit
ATATAGAGCTGAGGCGGTGTCGAGAAGGTCTCGAATAGAAGGAAAGAAGT6750.12672010813449228No Hit
ATGTTAACATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC6730.12634464114742713No Hit
GCACCGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG6670.12521824018623162No Hit
ATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGTCTGGA6600.12390410573150355No Hit
CCCTAACAGTAAATGAAAACAGGAGATTAAAATTAATTATGCCTGCTAGA6540.12277770477030807No Hit
GCTTTATTCTTCTTCTGTACCTAACTTTAATCCTGATTGGCAAACTCCTT6520.12240223778324291No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTTCTGTACCTAAC6510.12221450428971031No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCTCTCAGGCA6310.1184598344190587No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACACCACCACTTT6310.1184598344190587No Hit
GTTGATAGGTGTAAGAACTTTGTGGGACCCCTAACAGTAAATGAAAACAG6310.1184598344190587No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6290.11808436743199353No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG6240.11714569996433062No Hit
GCTTACTACTAATTCCCTGGATGCTGGGTCGTCCAAATTACTTCCCACCC6210.11658249948373291No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACCACC6180.11601929900313517No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGTGGATTGGGGACAGAAAGAT6180.11601929900313517No Hit
GGATAATATGGTTTGATTCCTTTATCTAAGGGCAAGTATTTGGTAACATT6110.11470516454840708No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG6060.11376649708074418No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACAAAATGCC6030.11320329660014644No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTTCTG6000.1126400961195487No Hit
GTATACATGCATATAAAGGCATTAAAGCGGGATATCCACATTGTGTAAAA5970.11207689563895096No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGACAACACGTAGCGCC5940.11151369515835322No Hit
CAAGATCTACAGCATGGGAGGTTGGTCTTCAAAACCTCGAAAAGGCATGG5930.11132596166482062No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5860.11001182721009255No Hit
ACCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGCACGACAGAAT5840.10963636022302739No Hit
CTTCAAAGACTGTGTGTTTGCTGAGTGGGAGGAGTTGGGGGAGGAGATTA5830.1094486267294948No Hit
GCTCAGAATACTGCCTCTGCCATATCGTCAACCTTCTCGAAGACTGGGGA5810.10907315974242963No Hit
GGAACATATTGTACAAAAAATCAAATTATGTTTTAGGAAACTTCCTGTAA5760.10813449227476675No Hit
ATGCTGGGTCGTCCAAATTACTTCCCACCCAGGTGGCTAGATTCATCAAC5740.10775902528770158No Hit
CTGTTAGGGGTCCCACAAAGTTCTTACACCTATCAACAATGTCCTCCTGC5700.10700809131357127No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCGGTG5590.10494302288471286No Hit
CCTTTATCTAAGGGCAAGTATTTGGTAACATTGGGATAAAATCTAGCAGG5580.10475528939118028No Hit
CTCCTAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGACCCGTAT5550.10419208891058254No Hit
GCTCTGGATAATGTGGTTTGATTCCTTTATCTAAGGGCAAGTATTTGGTA5530.10381662192351737No Hit
ACATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT5530.10381662192351737No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5480.10287795445585446No Hit
GTTTACAGGAAGTTTCCTAAAACATAATTTGATTTTTTGTACAATATGTT5390.10118835301406123No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGATACTGTTCT5390.10118835301406123No Hit
CCAAAAGACACCAAATATTCAAGAACAGTATCTCTTCCAAAAGTAAGACA5340.10024968554639833No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)