FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences467713
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22780.48705081962656593No Hit
CTCTAGAACAGTGTCTCTTCCAAAAGTGAGACAGGAAATGTGAAACCACA13180.2817967428743695No Hit
GTTAATATTCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC12920.2762377782956642No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC11920.2548571453006438No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCA10220.21851006920910898No Hit
ATTTACAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAATATGTT10020.2142339426101049No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA9430.20161936914304285No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9200.19670182355418817No Hit
GAATACTAACATTGGGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGA9040.19328092227498486No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG8550.18280441210742485No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8280.17703164119876935No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAATATGTT8180.1748935778992673No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT7980.17061745130026318No Hit
GTTCTAGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTATCGC7900.16890700066066155No Hit
GGATATTATCAACAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATG7870.16826558167081096No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTGTTGATAATATCCTCTTTC7770.1661275183713089No Hit
GTGTAAAAGGGGCGGCAAAGCCCAGAAGACCCACAATTCTTTGACATACT7630.16313422975200603No Hit
GGATAATAAGGTTTAATGCCTTTCTCTAAGGGCAAATATTTGGTAAGGTT7380.15778907150325094No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGAGAAAGGCATTAAACCTTAT7370.1575752651733007No Hit
ATTCTATGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA7210.15415436389409745No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCATTAAC7100.1518024942646452No Hit
TACCCTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6690.1430364347366868No Hit
GTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG6580.14068456510723457No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC6390.13662224483818067No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTCTCTAAGGGCAAATATTTGGTA6040.12913902328992352No Hit
CTCCAAAAGACACCAAATACTCTAGAACAGTGTCTCTTCCAAAAGTGAGA5850.12507670302086962No Hit
ATTATCAACAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAA5730.12251102706146719No Hit
CCTATCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5690.12165580174166636No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT5580.11930393211221411No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTTTCCATGC5410.11566922450306065No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAT5380.11502780551321003No Hit
CTTTTACACAATGTGGTTACCCAGCATTAATGCCTTTATATGCATGTATA5330.113958773863459No Hit
GTAGTAAGCTATGTCAATGTTAACATGGGCCTAAAAATCAGACAACTATT5280.11288974221370798No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATGACATATCCCATGAA5250.11224832322385737No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC5240.11203451689390716No Hit
CATTTAAACCCTAATAAGACCAAACGTTGGGGCTACTCCCTTAACTTCAT5100.1090412282746043No Hit
GCTTCGCTTGTATACATGCATATAAAGGCATTAATGCTGGGTAACCACAT5090.1088274219446541No Hit
CCCTTAACTTCATGGGATATGTCATTGGAAGTTGGGGTACCTTGCCACAG5070.10839980928475368No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACTGCCATG4880.1043374890156998No Hit
GAATATTAACATTGGGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4870.10412368268574959No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT4830.10326845736594878No Hit
CTATATGTTCAGGATAATAAGGTTTAATGCCTTTCTCTAAGGGCAAATAT4790.10241323204614795No Hit
GTTTAATGCCTTTCTCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAAC4720.10091658773649652No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACA4680.1000613624166957No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)