FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490515

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490515
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences467713
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22630.4838437246773128No Hit
CTCTAGAACAGTGTCTCTTCCAAAAGTGAGACAGGAAATGTGAAACCACA14630.3127986607171492No Hit
GTTAATATTCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC11710.25036721237168946No Hit
ATTTACAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAATATGTT10240.21893768186900942No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCA10220.21851006920910898No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC10200.2180824565492086No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9430.20161936914304285No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG9350.19990891850344122No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA9050.19349472860493508No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8730.18665292604652856No Hit
GAATACTAACATTGGGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGA8680.1855838943967775No Hit
GTTCTAGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTATCGC8470.18109396146782322No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTGTTGATAATATCCTCTTTC7990.1708312576302134No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAATATGTT7830.16741035635101015No Hit
GGATATTATCAACAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATG7640.16334803608195625No Hit
GTGTAAAAGGGGCGGCAAAGCCCAGAAGACCCACAATTCTTTGACATACT7640.16334803608195625No Hit
GGATAATAAGGTTTAATGCCTTTCTCTAAGGGCAAATATTTGGTAAGGTT7640.16334803608195625No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT7520.16078236012255379No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCATTAAC7300.1560786208636493No Hit
GTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG6950.14859539931539212No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGAGAAAGGCATTAAACCTTAT6900.14752636766564112No Hit
ATTCTATGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA6800.14538830436613906No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTCTCTAAGGGCAAATATTTGGTA6670.1426088220767864No Hit
CTCCAAAAGACACCAAATACTCTAGAACAGTGTCTCTTCCAAAAGTGAGA6650.142181209416886No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC5780.1235800587112182No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT5730.12251102706146719No Hit
CCTATCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5480.11716586881271207No Hit
TACCCTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5390.11524161184316023No Hit
GCTTCGCTTGTATACATGCATATAAAGGCATTAATGCTGGGTAACCACAT5340.11417258019340921No Hit
CTTTTACACAATGTGGTTACCCAGCATTAATGCCTTTATATGCATGTATA5310.1135311612035586No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACTGCCATG5250.11224832322385737No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAT5200.11117929157410635No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACA5170.11053787258425572No Hit
GTAGTAAGCTATGTCAATGTTAACATGGGCCTAAAAATCAGACAACTATT5010.10711697130505245No Hit
CTATATGTTCAGGATAATAAGGTTTAATGCCTTTCTCTAAGGGCAAATAT4970.10626174598525163No Hit
ATTATCAACAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAA4950.10583413332535122No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATGACATATCCCATGAA4930.10540652066545082No Hit
CTTATAAGGCCTTTCTGTGTAAACAATATCTGAACCTTTACCCCGTTGCT4770.10198561938624755No Hit
CCCTTAACTTCATGGGATATGTCATTGGAAGTTGGGGTACCTTGCCACAG4740.10134420039639694No Hit
GTTTAATGCCTTTCTCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAAC4710.10070278140654632No Hit
GAATATTAACATTGGGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4680.1000613624166957No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)