FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490516

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490516
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences406858
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCTAACCATACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT20710.5090228040249914No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19350.47559590815468783No Hit
GTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTGTGTTCAGG17870.439219580295828No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCA15900.39079973848369703No Hit
CTCTTACTGTTAATGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG14940.3672042825752474No Hit
GGATATTATCAACAGATGTCAACAATATGTGGGCCCTCTTACTGTTAATG14180.34852454664772475No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC11990.294697412856574No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11290.2774923929233295No Hit
GTTCTATCCTAACCATACCAAATATTTGCCCTTAGATAAAGGCATTAAAC11150.27405138893668063No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA10070.2475065010396748No Hit
CTCTAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA9720.23890399107305252No Hit
TCCACAGAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTG9690.23816663307591346No Hit
ATTATCAACAGATGTCAACAATATGTGGGCCCTCTTACTGTTAATGAAAA9560.2349714150883109No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCATTAAC9530.23423405709117184No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9410.23128462510261563No Hit
CTCCAAAAGACACCAAATACTCTAGAACAGTTTCTCTTCCAAAAGTAAGA9150.22489418912741052No Hit
GTAGTAAGCTATGTCAATGTTAACATGGGCCTAAAACTCAGACAAATATT8920.21924111114934447No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTATGGTT8430.2071975971960733No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC8410.20670602519798062No Hit
GAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTGTGTTCA8140.2000698032237292No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTT8000.19662879923708024No Hit
CAGTAAGAGGGCCCACATATTGTTGACATCTGTTGATAATATCCTCTTTC7660.18827207526950435No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC7510.1845852852838091No Hit
ATGATTAACTGTGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGG7490.18409371328571641No Hit
GGGTAAAGGTTCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7320.17991535130192843No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG7220.17745749131146493No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT7060.17352491532672332No Hit
CTTTTACACAATGTGGTTACCCTGCATTAATGCCTTTATATGCATGTATA6860.16860919534579633No Hit
GGAACATATTGTACAAAAATTGAAACAATGTTTTCGGAAACTTCCCATAA6830.16787183734865727No Hit
ATTTATGGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAATATGTT6530.16049825737726675No Hit
GTCTATTTATGGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAATA6420.1577946113877569No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA6350.15607410939443245No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGT6250.15361624940396895No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT6240.1533704634049226No Hit
GATTAACTGTGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC6180.15189574741064452No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG6100.1499294594182737No Hit
GTGTAAAAGGGGCGGCAAAGCCCAGAAGACCCACAATTCTTTGACAGACT6090.14968367341922734No Hit
GTATACATGCATATAAAGGCATTAATGCAGGGTAACCACATTGTGTAAAA5990.14722581342876384No Hit
ATGTTAACATGGGCCTAAAACTCAGACAAATATTGTGGTTTCACATTTCC5840.1435390234430686No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5450.13395336948026093No Hit
GGTTCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG5310.130512365493612No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA5180.12731714750600945No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC5140.12633400350982407No Hit
CTCCATATTCGGTGCAGGGACCCCAGTCCTCGAGAAGATTGACGATATGG5130.1260882175107777No Hit
TTATTATCCTGAACACACAGTTAATCATTACTTCCAAACTAGACATTATT5080.12485928751554595No Hit
GTTCTAGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTACCGC4810.11822306554129451No Hit
CTTCAAAGACTGTGTATTTAAGGACTGGGAGGAGTTGGGGGAGGAGACTA4690.11527363355273829No Hit
ACTGACAACAGTGCCAGCAACGCCTCCTCCTGCCTCCACCAATCGGCAGT4690.11527363355273829No Hit
GGCCAAGACTGTCCACCATCTTGAGTCCCTTTTTACCTCTATTACCAATT4650.1142904895565529No Hit
GTTGATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCC4610.1133073455603675No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC4580.11256998756322845No Hit
GTCTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTG4480.11011212757276495No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGACATG4460.10962055557467223No Hit
GCATTAATGCCTTTATATGCATGTATACAAGCTAAGCAGGCTTTCACTTT4410.10839162557944049No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCT4400.10814583958039414No Hit
ATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTGTGTTCAGGAT4380.10765426758230145No Hit
GTTCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA4360.10716269558420874No Hit
CATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAG4330.10642533758706968No Hit
GGAATATGGTGACCCACAAAATGAGGCACTGCGTGTAGTTTCTCTCTTAT4210.10347590559851348No Hit
TCACCATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGA4200.10323011959946715No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACA4190.10298433360042078No Hit
GAATTAGTAGTAAGCTATGTCAATGTTAACATGGGCCTAAAACTCAGACA4180.10273854760137442No Hit
GTTAGTATCCCTTGGACTCACAAGGTGGGAAACTTTACTGGGCTTTATTC4160.10224697560328173No Hit
GCGTGTAGTTTCTCTCTTATATAAAATACCAGCCTTCCACAGAGTATGTA4070.1000349016118646No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)