FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences406858
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19220.47240069016708536No Hit
GTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTGTGTTCAGG18770.4613403202099996No Hit
ATCCTAACCATACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT16490.4053011124274317No Hit
CTCTTACTGTTAATGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG13910.3418883246734733No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCA13880.34115096667633427No Hit
GGATATTATCAACAGATGTCAACAATATGTGGGCCCTCTTACTGTTAATG13700.3367268186934999No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC11390.27995025291379305No Hit
GTTCTATCCTAACCATACCAAATATTTGCCCTTAGATAAAGGCATTAAAC10320.2536511510158335No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9880.24283656705779413No Hit
CTCTAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA9820.24136185106351601No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9170.22538576112550324No Hit
ATTATCAACAGATGTCAACAATATGTGGGCCCTCTTACTGTTAATGAAAA8820.21678325115888097No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCATTAAC8450.20768916919416602No Hit
GAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTGTGTTCA8330.2047397372056098No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA8290.2037565932094244No Hit
CTCCAAAAGACACCAAATACTCTAGAACAGTTTCTCTTCCAAAAGTAAGA8250.20277344921323903No Hit
TCCACAGAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTG8230.2022818772151463No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTATGGTT8060.19810351523135836No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC7980.19613722723898758No Hit
GTAGTAAGCTATGTCAATGTTAACATGGGCCTAAAACTCAGACAAATATT7740.19023836326187515No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTT7700.18925521926568975No Hit
CAGTAAGAGGGCCCACATATTGTTGACATCTGTTGATAATATCCTCTTTC7500.18433949928476273No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC7290.17917799330478937No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG6840.16811762334770364No Hit
GGGTAAAGGTTCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6790.16688869335247186No Hit
GTGTAAAAGGGGCGGCAAAGCCCAGAAGACCCACAATTCTTTGACAGACT6680.16418504736296202No Hit
ATGATTAACTGTGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGG6440.1582861833858496No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG6400.1573030393896642No Hit
GTCTATTTATGGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAATA6350.15607410939443245No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT6300.1548451793992007No Hit
GTATACATGCATATAAAGGCATTAATGCAGGGTAACCACATTGTGTAAAA6050.14870052942304196No Hit
ATTTATGGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAATATGTT6030.14820895742494924No Hit
GGAACATATTGTACAAAAATTGAAACAATGTTTTCGGAAACTTCCCATAA5880.14452216743925397No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGT5860.14403059544116129No Hit
CTTTTACACAATGTGGTTACCCTGCATTAATGCCTTTATATGCATGTATA5820.1430474514449759No Hit
GATTAACTGTGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC5340.13124972349075106No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA5290.13002079349551932No Hit
GGTTCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG5150.12657978950887042No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT5110.125596645512685No Hit
CTCCATATTCGGTGCAGGGACCCCAGTCCTCGAGAAGATTGACGATATGG4980.12240142752508246No Hit
GGCCAAGACTGTCCACCATCTTGAGTCCCTTTTTACCTCTATTACCAATT4910.12068092553175801No Hit
ATGTTAACATGGGCCTAAAACTCAGACAAATATTGTGGTTTCACATTTCC4890.12018935353366532No Hit
CTTCAAAGACTGTGTATTTAAGGACTGGGAGGAGTTGGGGGAGGAGACTA4820.11846885154034084No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCT4780.11748570754415545No Hit
GGAATATGGTGACCCACAAAATGAGGCACTGCGTGTAGTTTCTCTCTTAT4720.11601099154987735No Hit
TTATTATCCTGAACACACAGTTAATCATTACTTCCAAACTAGACATTATT4710.115765205550831No Hit
GAATTAGTAGTAAGCTATGTCAATGTTAACATGGGCCTAAAACTCAGACA4580.11256998756322845No Hit
GTCTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTG4530.1113410575679967No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA4530.1113410575679967No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACAG4520.11109527156895034No Hit
GTTCTAGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTACCGC4510.11084948556990398No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA4510.11084948556990398No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4500.11060369957085765No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG4380.10765426758230145No Hit
GTTGATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCC4360.10716269558420874No Hit
CCCTTGGACTCACAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG4320.10617955158802334No Hit
ATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTGTGTTCAGGAT4290.10544219359088429No Hit
GCGTGTAGTTTCTCTCTTATATAAAATACCAGCCTTCCACAGAGTATGTA4280.10519640759183793No Hit
ATGTGGTATTGGGGGCCAAGACTGTCCACCATCTTGAGTCCCTTTTTACC4280.10519640759183793No Hit
GTTCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA4250.1044590495946989No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC4240.10421326359565254No Hit
ACTGACAACAGTGCCAGCAACGCCTCCTCCTGCCTCCACCAATCGGCAGT4180.10273854760137442No Hit
GCCTTCCACAGAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAAC4100.10077225960900363No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)