FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490518

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490518
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences364864
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17590.4820974390457814No Hit
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT16070.4404380810384143No Hit
ATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACAGTAAATGAAAAGAG14470.39658612524118575No Hit
CCTATTAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT11810.32368224872829326No Hit
TTATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACAGTAAATGAAAAG8960.24557095246447994No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACC8770.240363532713559No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA8710.23871908437116296No Hit
CTCTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT8440.23131906683038064No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT8190.22446719873706367No Hit
ATATAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC7940.21761533064374672No Hit
ATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCCATGAA7850.21514865813015263No Hit
TATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCT7800.2137782845114892No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAGATATTTAGTGTGGGT7620.208844939484301No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAAATC7580.20774864058937026No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG7440.20391159445711277No Hit
GGATCATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA7230.19815602525872655No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC7200.1973338010875285No Hit
GTACGGGACCATGCAAAACTTGCACGACTCCTGCTCAAGGCAACTCTATG7150.1959634274688651No Hit
CACTAAATATCTGCCCTTAGACAAAGGAATTAAACCTTATTATCCAGATC7000.19185230661287492No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6850.18774118575688475No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAA6590.1806152429398351No Hit
AGTCTGTACAGCATCGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT6510.1784226451499737No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6480.17760042097877565No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGG6430.17623004736011225No Hit
ATTCTATCCTACCCACACTAAATATCTGCCCTTAGACAAAGGAATTAAAC6340.17376337484651816No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT6340.17376337484651816No Hit
GATATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAAT6270.17184485178038938No Hit
GTCTAAGGGCAGATATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAA6110.16745965620066655No Hit
GGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTAC6030.1652670584108051No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA6020.16499298368707244No Hit
CTAAATATCTGCCCTTAGACAAAGGAATTAAACCTTATTATCCAGATCAG5930.16252631117347835No Hit
ATGTTAATACTAACATGGGTTTAAAGATCAGGCAACTATTGTGGTTTCAT5810.1592374144886862No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGTCACCCGTG5700.1562225925276267No Hit
GTATACATGCATACAAAGGCATCAAGGCAGGATATCCACATTGTGTAAAT5270.14443737940712156No Hit
GTACAATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCC4940.13539291352394317No Hit
TATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCT4810.13182994211541835No Hit
CCCTTAGACAAAGGAATTAAACCTTATTATCCAGATCAGGTAGTTAATCA4790.131281792667953No Hit
CTATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATA4710.12908919487809156No Hit
TTATTATCCAGATCAGGTAGTTAATCATTACTTCCAAACCAGACATTATT4690.12854104543062622No Hit
TTCTAAAACAGTGTTTGATCTTTTGTACAATATGATCCTGTGGCAATGTT4620.12662252236449748No Hit
ATTGATAGGTGTCAACAATTTGTGGGCCCTCTCACAGTAAATGAAAAGAG4540.12442992457463603No Hit
GTCTTATATAAGAGGACTCTTGGACTCCCAGCAATGTCAACGACCGACCT4520.12388177512717066No Hit
CTCCGAAAGAGACCAAATATTCAATTACAGTCTCTCTTCCAAAAGTAAGG4500.12333362567970531No Hit
ATTCCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTT4500.12333362567970531No Hit
CAATTACAGTCTCTCTTCCAAAAGTAAGGCAAGATGTATGAAACCACAAT4300.11785213120505175No Hit
CCATTTACACAATGTGGATATCCTGCCTTGATGCCTTTGTATGCATGTAT4230.11593360813892299No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTGTTTGATCTTTTGT4190.11483730924399227No Hit
GATCTGGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAGATATTTAGTG4150.11374101034906157No Hit
ATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGG4090.1120965620066655No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTCATGACTTCTATCCTTCCGTCAG4060.11127433783546746No Hit
ATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAG4050.11100026311173478No Hit
TTCCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTC4010.10990396421680408No Hit
CTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAGATTCATTTACAAG3990.10935581476933871No Hit
ATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTTGGA3900.1068891422557446No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT3880.10634099280827924No Hit
ATCCTACCCACACTAAATATCTGCCCTTAGACAAAGGAATTAAACCTTAT3860.10579284336081389No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTCCGCGAAAGGACGACAGAAT3860.10579284336081389No Hit
GGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCA3740.10250394667602175No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)